Time of Ovulation in the Beagle Bitch1

Phemister, Robert D.; Holst, P. A.; Spano, J. S.; Hopwood, M. L.

doi:10.1093/biolreprod/8.1.74

Cited by 117 publications

(57 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is not likely that this difference in variation is caused by the different ways of defining the length of gestation since the rise in progesterone concentration in peripheral blood is closely related to the pre-ovulatory LH peak (1,7,8). Instead, we suggest that the greater variation in the length of gestation in our study was primarily caused by the difference in litter size.…”

Section: Discussionmentioning

confidence: 60%

Influence of litter size and breed on variation in length of gestation in the dog

Okkens

Hekerman

Vogel³

et al. 1993

Veterinary Quarterly

View full text Add to dashboard Cite

SUMMARYThe variation in the length of gestation, the period from mating until parturition, was studied in 77 dogs of different breeds; the time for mating was determined by measuring peripheral blood progesterone levels. The mean length of gestation was 62.1 ± 0.2 (S.E.M.) days, with a variation of 11 days. The number of pups appeared to influence the length of gestation. Length of gestation was negatively correlated (r = -0.96, P < 0.001, n = 44) with litter size in litters with 7 or fewer pups. The intra-breed variation in length of gestation in the five breeds represented by five or more bitches was 3 -6 days. The mean gestation of Alsatians (60.1 ± 0.5, n = 9) was shorter (P < 0.005) than that of the other breeds combined (62.3 ± 0.3, n = 68). The primiparous/multiparous status of the bitch did not influence the length of gestation.

show abstract

Section: Discussionmentioning

confidence: 60%

Influence of litter size and breed on variation in length of gestation in the dog

Okkens

Hekerman

Vogel³

et al. 1993

Veterinary Quarterly

View full text Add to dashboard Cite

show abstract

“…Ovulation occurs 2 or 3 days after the onset of estrus in most bitches [29,38]. The eggs are ovulated as primary oocytes and are incapable of being fertilized until at least 48 hr later when they become secondary oocytes in the oviduct [15,40].…”

mentioning

confidence: 99%

Induction of Dog Sperm Capacitation by Oviductal Fluid.

Kawakami

Hori

Tsutsui

1998

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Four estrous beagles were inseminated with 1 × 10 8 sperm into both the right and left uterine horns, and the uterine horn and oviduct on one side were removed under anesthesia after 7 hr and 24 hr, respectively. The lumen of the uterine horns and oviducts was flushed with canine capacitation medium (CCM), and movement of the sperm in CCM was assessed by phase-contrast microscopy. In a second experiment, ejaculated sperm obtained from 5 normal beagles was incubated in CCM supplemented with oviductal flush fluid (OF-CCM) at 38°C with 5% CO 2 in air. Motility of sperm, and percentages of hyperactivated sperm (%HA) and acrosome-reacted sperm (%AR) among freely swimming (FS) sperm were investigated until 24 hr after the start of incubation. After 7 hr of incubation the sperm was coincubated with canine oocytes in OF-CCM for 2 hr, and the number of zona pellucida-binding (zona-binding) sperm was then counted. The %HA among the sperm in the oviductal flush fluid both 7 hr (mean ± S.E.; 15.0 ± 2.4%) and 24 hr (77.5 ± 5.2%) after intrauterine insemination were significantly higher than in the uterine flush fluid (P<0.05, 0.01, respectively). The motility and %HA among FS-sperm in OF-CCM were higher than in the control medium without oviductal fluid. However, there was no difference in the %AR between OF-CCM and control medium. The number of zona-binding sperm in OF-CCM (8 ± 1) was significantly greater than in control medium (5 ± 1) (P<0.05). These results suggest that oviductal fluid in the estrous bitch maintains sperm motility and induces sperm capacitation. -KEY WORDS: canine, oviductal fluid, sperm capacitation.J. Vet. Med. Sci. 60(2): 197-202, 1998 and four beagles in the estrous period, aged 2-5 years, were used in this experiment. The optimum time for intrauterine insemination in the estrous bitches was determined by daily examination of receptivity for a male dog after vaginal bleeding. Four anestrous bitches ovariohysterectomized at the teaching hospital of our university were used for the zona pellucida-binding assay.Semen collection and evaluation: The sperm-rich second fraction of ejaculated semen [14] was collected by digital manipulation and immediately transported to the laboratory. The concentration of sperm in the semen was determined by hematocytometer counts. The percentage of motile sperm was estimated by using a sperm motility examination plate (Fujihira Industry Inc., Tokyo), and semen samples with good sperm motility (>90%) were used.Intrauterine insemination and flushing of uteri and oviducts: Intrauterine insemination was performed on four estrous bitches on the 4th or 5th day after the start of acceptance, as described in the report by Tsutsui et al. [41]. All of the bitches were laparotomized under halothane inhalation anesthesia. A 20-gauge retaining needle connected to a 1 ml injection syringe was inserted into the middle portion of the right and left uterine horns, and semen adjusted to a concentration of 1 × 10 8 sperm/0.3 ml seminal plasma collected from one of four dogs was...

show abstract

“…The dog might be one of the most important mammalian species, not only as a companion animal but also for its unique reproductive system [1][2][3].…”

mentioning

confidence: 99%