Time-resolved fluorescence lifetime for cutaneous melanoma detection

Abstract

Melanoma is the most aggressive skin cancer type. It is characterized by pigmented lesions with high tissue invasion and metastatic potential. The early detection of melanoma is extremely important to improve patient prognosis and survival rate, since it can progress to the deadly metastatic stage. Presently, the melanoma diagnosis is based on the clinical analysis of the macroscopic lesion characteristics such as shape, color, borders following the ABCD rules. The aim of this study is to evaluate the time-resolved fluorescence lifetime of NADH and FAD molecules to detect cutaneous melanoma in an experimental in vivo model. Forty-two lesions were analyzed and the data was classified using linear discriminant analysis, a sensitivity of 99.4%, specificity of 97.4% and accuracy of 98.4% were achieved. These results show the potential of this fluorescence spectroscopy for melanoma detection.

Time-resolved fluorescence spectroscopy for clinical diagnosis of actinic cheilitis

“…The output signal of the hybrid PMT was read using a Time-Correlated Single Photon Counting (TCSPC) board (SPC-150, Becker and Hickl, Berlin, Germany). More detailed information is published elsewhere [20,30].…”

“…Fiber-bundle spectroscopy systems are well suited for skin and oral investigation, due to their feasibility. They have already been used for melanoma [20], basal cell carcinoma [21], in situ oral carcinoma [22] and skin photoaging [23]. Small fiber dimension allows to use them also for non-invasive inspection in hollow core organs as colon [24], thyroid [25], or vascular systems [26,27].…”

Significance of Discriminant Analysis in Prediction of Pregnancy in IVF Treatment

“…This method was used to find a set of variables that might differentiate normal skin from skin with cutaneous melanoma, offering a possibility for detection of skin melanoma (Pires et al, 2014). Discriminant analysis can be useful in other branches of oncology as well, for example in gynecological oncology.…”

Simultaneous detection of tissue autofluorescence decay distribution and time-gated photo-bleaching rates

“…Biological and biomedical applications of the autofluorescence spectroscopy have been successfully applied in cell biology and clinical diagnostics for detection of abnormal tissues [1][2][3][4][5]. The most important endogenous fluorophores are molecules widely distributed in cells and tissues, like proteins containing aromatic amino-acids, NAD(P)H, flavins and lipo-pigments [6,7].…”