Tissue-based Quantification of 8-Hydroxy-2′-Deoxyguanosine in Human Prostate Biopsies Using Quantitative Fluorescence Imaging Analysis

Richardson, Tim; McCanse, Webb; Casale, George P.; Huang, Dali; Tian, Jennifer; Elkahwaji, Johny E.; Lele, Subodh M.; Hemstreet, George P.

doi:10.1016/j.urology.2009.01.052

Cited by 8 publications

(3 citation statements)

References 18 publications

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“…Some are practical for slide-mounted FFPE tissue, such as quantitative fluorescence imaging analysis (QFIA), which is reproducible and sensitive for specific standardized proteins, 12−14 or MALDI-imaging mass spectrometry (MS). 15−17 Other encouraging mass spectrometry (MS)-based proteomic studies of FFPE tissues have appeared in the recent literature; 2−7,9,18−20 however, these investigations have typically been restricted to minute tissue specimens, such as those obtained by laser capture microdissection.…”

Section: Introductionmentioning

confidence: 99%

“…A variety of methods for profiling FFPE tissue have been employed recently. Some are practical for slide-mounted FFPE tissue, such as quantitative fluorescence imaging analysis (QFIA), which is reproducible and sensitive for specific standardized proteins, − or MALDI-imaging mass spectrometry (MS). − Other encouraging mass spectrometry (MS)-based proteomic studies of FFPE tissues have appeared in the recent literature; − ,,− however, these investigations have typically been restricted to minute tissue specimens, such as those obtained by laser capture microdissection. Further, some studies report high rates of false-positive protein identification and are limited to the analysis of tryptic digests of FFPE tissues by liquid chromatography–MS (LC–MS).…”

Section: Introductionmentioning

confidence: 99%

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Pressure-Assisted Protein Extraction: A Novel Method for Recovering Proteins from Archival Tissue for Proteomic Analysis

et al. 2012

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Formaldehyde-fixed, paraffin-embedded (FFPE) tissue repositories represent a valuable resource for the retrospective study of disease progression and response to therapy. However, the proteomic analysis of FFPE tissues has been hampered by formaldehyde-induced protein modifications, which reduce protein extraction efficiency and may lead to protein misidentification. Here, we demonstrate the use of heat augmented with high hydrostatic pressure (40,000 psi) as a novel method for the recovery of intact proteins from FFPE mouse liver. When FFPE mouse liver was extracted using heat and elevated pressure, there was a 4-fold increase in protein extraction efficiency, a 3-fold increase in the extraction of intact proteins, and up to a 30-fold increase in the number of nonredundant proteins identified by mass spectrometry, compared to matched tissue extracted with heat alone. More importantly, the number of nonredundant proteins identified in the FFPE tissue was nearly identical to that of matched fresh-frozen tissue.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pressure-Assisted Protein Extraction: A Novel Method for Recovering Proteins from Archival Tissue for Proteomic Analysis

et al. 2012

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“…The interaction of HO • with the nucleobases of the DNA strand, such as guanine, leads to the formation of C8-hydroxyguanine (8-OHGua) or its nucleoside form deoxyguanosine (8-hydroxy-2’-deoxyguanosine; (8-OHdG) [12, 13]. Determination and analysis of 8-OHdG can be performed in animal organs and in human samples (urine, human organs, and leukocyte DNA).…”

Section: Introductionmentioning

confidence: 99%

Urine markers and prostate cancer

Wilkosz¹,

Bryś²,

Różański³

2011

cejurol1/2011

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Prostate cancer (PCa) is globally the most common cancer in men, with an estimated prevalence of more than two million cases. Given the poor success rate in treating advanced PCa, intervention in early stages may reduce the progression of a small, localized carcinoma to a large metastatic lesion, thereby reducing disease-related deaths. Urine is readily available and can be used to detect either exfoliated cancer cells or secreted products. The major advantages of urine-based assays are their noninvasive character and ability to monitor PCa with heterogeneous foci. The aim of this review was to summarize the current evidence regarding performance characteristics of tests proposed for urine-based prostate cancer detection.

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