Tissue culture in &lt;i&gt; Pinus caribaea &lt;/i&gt; mor. var. &lt;i&gt; hondurensis &lt;/i&gt; barr &amp; golf. 1. effects of two auxins and two cytokinins on callus growth and greening

Akaneme, FI; Ene-Obong, E. E.

doi:10.4314/as.v4i1.1516

Cited by 4 publications

(4 citation statements)

References 28 publications

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“…The friability of callus tissue is highly desirable for establishing cell suspension culture [ 34 ]. Histological analysis is very useful to verify the process friability of the callus.…”

Section: Discussionmentioning

confidence: 99%

Production of Secondary Metabolites from Cell Cultures of Sageretia thea (Osbeck) M.C. Johnst. Using Balloon-Type Bubble Bioreactors

Kim

Han

Murthy

et al. 2023

Plants

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Sageretia thea is used in the preparation of herbal medicine in China and Korea; this plant is rich in various bioactive compounds, including phenolics and flavonoids. The objective of the current study was to enhance the production of phenolic compounds in plant cell suspension cultures of Sageretia thea. Optimum callus was induced from cotyledon explants on MS medium containing 2,4-dichlorophenoxyacetic acid (2,4-D; 0.5 mg L−1), naphthalene acetic acid (NAA, 0.5 mg L−1), kinetin (KN; 0.1 mg L−1) and sucrose (30 g L−1). Browning of callus was successfully avoided by using 200 mg L−1 ascorbic acid in the callus cultures. The elicitor effect of methyl jasmonate (MeJA), salicylic acid (SA), and sodium nitroprusside (SNP) was studied in cell suspension cultures, and the addition of 200 µM MeJA was found suitable for elicitation of phenolic accumulation in the cultured cells. Phenolic and flavonoid content and antioxidant activity were determined using 2,2 Diphenyl 1 picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethybenzothiazoline-6-sulphonic acid (ABTS), ferric reducing antioxidant power (FRAP) assays and results showed that cell cultures possessed highest phenolic and flavonoid content as well as highest DPPH, ABTS, and FRAP activities. Cell suspension cultures were established using 5 L capacity balloon-type bubble bioreactors using 2 L of MS medium 30 g L−1 sucrose and 0.5 mg L−1 2,4-D, 0.5 mg L−1 NAA, and 0.1 mg L−1 KN. The optimum yield of 230.81 g of fresh biomass and 16.48 g of dry biomass was evident after four weeks of cultures. High-pressure liquid chromatography (HPLC) analysis showed the cell biomass produced in bioreactors possessed higher concentrations of catechin hydrate, chlorogenic acid, naringenin, and other phenolic compounds.

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“…The friability of callus tissue is highly desirable for establishing cell suspension culture [ 34 ]. Histological analysis is very useful to verify the process friability of the callus.…”

Section: Discussionmentioning

confidence: 99%

Production of Secondary Metabolites from Cell Cultures of Sageretia thea (Osbeck) M.C. Johnst. Using Balloon-Type Bubble Bioreactors

Kim

Han

Murthy

et al. 2023

Plants

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“…In Pinus caribaea Morelet it was found that development of certain calli and plant regeneration depended on specific auxin/ cytokinin interactions: NAA/6-furfuryl aminopurine (kinetin) in the medium produced 100% friable calli without influence of the NAA/kinetin ratio. However, for NAA and BAP cultures it was necessary to have a 1:2 NAA/BAP ratio to obtain the same results (Akaneme and Eneobong, 2008). Peres and Kerbauy (1999) found that alteration in endogenous auxin/cytokinin ratio, favouring cytokinins, strongly influenced plant development under in vitro conditions.…”

Section: Resultsmentioning

confidence: 99%

Management of Auxin-Cytokinin Interactions to Improve Micropropagation Protocol of Henequen (Agave fourcroydes Lem.)

Caraballo

Oramas

García

et al. 2010

Chilean J. Agric. Res.

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Henequen (Agave fourcroydes Lem.) is a high value plant species both for agricultural and ecological uses. The species is very well known worldwide by the quality of its fiber that is widely used for industrial purposes. This species is propagated at a large scale by asexual methods but current propagation technologies are not satisfying the grower's demands. A. fourcroydes has been cultivated in vitro but it has shown some recalcitrant behaviour during the multiplication and rooting stages. The combination of 6-benzylamynopurine (BAP) (0.75 mg L-1) and indolebutyric acid (IBA) (1.0 mg L-1) instead of 2,4-dichlorophenoxyacetic acid (2,4-D) significantly improved explant survival and shooting during the establishment of in vitro young shoots. Combining thidiazuron (TDZ) (0.5 or 0.75 mg L-1) with BAP (1.0 mg L-1) and IBA (1.0 mg L-1) in the basal medium increased the multiplication rate of henequen and significantly speeded out bud dormancy breaking. To improve rooting of the micropropagated shoots, the addition of IBA and naphthylacetic acid (NAA) was tested. The best rooting efficiency was obtained when the basal medium was supplemented with 0.5 or 0.75 mg L-1 of NAA, giving 100% of rooted explants and an average of 9.40 and 11.55 roots per explants, respectively. Over 94% of micropropagated plants survived the ex vitro weaning step and no morphological disorders were observed in any of the plants. Modification of plant growth regulators composition in the medium was a key factor to improve the efficiency of the micropropagation technology of henequen.

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“…Different types of plant growth regulators may be able to induce morphologically dissimilar calli from the same or different explants of the same plants (JayaSree et al 2001;Visarada et al 2002;Akaneme & Ene-Obong 2008). Here, it was found that callus initiation in the leaf and intermodal explants appeared to be similarly sensitive to the toxic effects of increasing concentrations of Cd in the respective media for induction of type-A or -B callus, suggesting that Cd availability might be similar in the respective culture media.…”

Section: Discussionmentioning

confidence: 98%

Differential cadmium resistance of two morphologically distinct types of potato (Solanum tuberosum) callus

et al. 2015

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Callus culture has been used to study the cellular basis of sensitivity of plants to toxic trace elements including cadmium. Callus friability may be related to plant response to exposure to trace elements as callus friability is related to the plant cell wall which plays a role in resistance of plant cells to trace element toxicity. The aim of this study was to investigate the relationship between two types of potato callus with different friability and their sensitivity to cadmium. A high frequency (about 80%) of leaf or internodal explants of potato (Solanum tuberosum L., cv, Iwa) formed a friable, pale green callus (type-A callus) on half-strength basal Murashige and Skoog medium supplemented with 12.42 µM picloram. On medium supplemented with 4.43 µM 6-benzyladenine (BA) and 5.37 µM 1-naphthalene acetic acid (NAA), a compact, non-friable callus (type-B callus) was induced in about 80% of the explants. Type-B callus was greener than type-A callus. Callus formation in both the leaf and internodal explants were completely inhibited on the medium used for induction of type-A or type-B callus when the respective medium was supplemented with 54 µM cadmium chloride (Cd). The type-B callus was found to be more resistant (less necrosis and higher relative growth rates) to 27, 54, and 109 µM of Cd than the type-A callus. The type-B callus also exhibited a higher level of peroxidase activity (a marker antioxidant enzyme counteracting oxidative stress) than the type-A callus when cultured on these Cd concentrations. This is the first study showing the importance of callus friability in plant cell response to Cd treatment.

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Tissue culture in <i> Pinus caribaea </i> mor. var. <i> hondurensis </i> barr & golf. 1. effects of two auxins and two cytokinins on callus growth and greening

Cited by 4 publications

References 28 publications

Production of Secondary Metabolites from Cell Cultures of Sageretia thea (Osbeck) M.C. Johnst. Using Balloon-Type Bubble Bioreactors

Production of Secondary Metabolites from Cell Cultures of Sageretia thea (Osbeck) M.C. Johnst. Using Balloon-Type Bubble Bioreactors

Management of Auxin-Cytokinin Interactions to Improve Micropropagation Protocol of Henequen (Agave fourcroydes Lem.)

Differential cadmium resistance of two morphologically distinct types of potato (Solanum tuberosum) callus

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