Tissue distribution of 20nm, 100nm and 1000nm fluorescent polystyrene latex nanospheres following acute systemic or acute and repeat airway exposure in the rat

Sarlo, Katherine; Blackburn, Karen; Clark, Edwin D.; Grothaus, Jeff; Chaney, Joel; Neu, Suzanne M.; Flood, Janine A.; Abbott, Dana; Bohne, Clarence; Casey, Keith; Fryer, Charles; Kuhn, Mike

doi:10.1016/j.tox.2009.07.002

Cited by 76 publications

(57 citation statements)

References 19 publications

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“…A wide variety of applications are being investigated for use in biomedical research 11 . This method could be utilized for assessment of different biological samples (such as various tissues types, bronchoalveolar lavage samples, and blood smears) that have been exposed to nanoparticles of a variety of elemental compositions [16][17][18][19] . Furthermore, this method is useful for studying nanoparticle biodistribution in vivo and in vitro, which is pertinent for nanoscale drug delivery studies 11 .…”

Section: Introductionmentioning

confidence: 99%

Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping

Roth

Peña

Neu‐Baker

et al. 2015

JoVE

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Nanomaterials are increasingly prevalent throughout industry, manufacturing, and biomedical research. The need for tools and techniques that aid in the identification, localization, and characterization of nanoscale materials in biological samples is on the rise. Currently available methods, such as electron microscopy, tend to be resource-intensive, making their use prohibitive for much of the research community. Enhanced darkfield microscopy complemented with a hyperspectral imaging system may provide a solution to this bottleneck by enabling rapid and less expensive characterization of nanoparticles in histological samples. This method allows for high-contrast nanoscale imaging as well as nanomaterial identification. For this technique, histological tissue samples are prepared as they would be for light-based microscopy. First, positive control samples are analyzed to generate the reference spectra that will enable the detection of a material of interest in the sample. Negative controls without the material of interest are also analyzed in order to improve specificity (reduce false positives). Samples can then be imaged and analyzed using methods and software for hyperspectral microscopy or matched against these reference spectra in order to provide maps of the location of materials of interest in a sample. The technique is particularly well-suited for materials with highly unique reflectance spectra, such as noble metals, but is also applicable to other materials, such as semi-metallic oxides. This technique provides information that is difficult to acquire from histological samples without the use of electron microscopy techniques, which may provide higher sensitivity and resolution, but are vastly more resource-intensive and time-consuming than light microscopy.

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Section: Introductionmentioning

confidence: 99%

Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping

Roth

Peña

Neu‐Baker

et al. 2015

JoVE

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“…They have potential to incorporate lipophilic or hydrophilic drugs or diagnostics (Muhlen et al, 1998;Utreja & Jain, 2002;Reddy et al, 2007). Intravenous studies show that liver is the major organ depot for nanoparticles followed by spleen for particles in the size range of 200-300 nm (Garnett & Kallinteri, 2006;De Jong et al, 2008;Sarlo et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Formulation and evaluation of gemcitabine-loaded solid lipid nanoparticles

Doijad

Shivakumar

et al. 2013

Drug Delivery

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Gemcitabine-loaded solid lipid nanoparticles (SLNs) were produced by double emulsification technique using stearic acid as lipid, soy lecithin as surfactant and sodium taurocholate as cosurfactant. Prepared nanoparticles are characterized for particle size and surface morphology using scanning electron microscopy (SEM). Particle yield, entrapment efficiency and zeta potential were also determined. In-vitro release studies were performed in phosphate-buffered saline (PBS) pH 7.4 using metabolic shaker. The formulation F6 with maximum entrapment efficiency 72.42% and satisfactory in-vitro release was selected. In-vivo tissue distribution to liver, spleen, lung, heart and kidneys of optimized formulation followed by stability study under specific conditions were also determined. This investigation has shown preferential drug targeting to liver followed by spleen, lungs, kidneys and heart. Stability studies showed no significant change in the particle size followed with very slight decrease in entrapment efficiency at 25 AE 2 C/60 AE 5% RH over a period of three months.

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“…15 Fluorescent polystyrene NPs have been employed in a number of scientific studies due to their similarities in size and zeta potential properties when compared to biocompatible polymeric particles proposed for biomedical diagnostic and drug delivery applications. 16 For example, fluorescent polystyrene NPs have been investigated in relation to aspiration into the airways of rats, 17 mucociliary clearance, 16 transport across rat and human pulmonary epithelial cells, 18 intravenous injection in rats, 17 distribution in the skin, 18,19 uptake in rat and human hepatocytes, 20 uptake in HeLa cells, 21 disposition in periocular and ocular rat tissue following subconjunctival injection, 22 and injection into extraembryonic mouse tissue. 23 In this work, we investigate the uptake and transport of fluorescent polystyrene NPs in BeWo b30 cells following model optimization specific to the application of NPs.…”

Section: Introductionmentioning

confidence: 99%

In vitro placental model optimization for nanoparticle transport studies

Cartwright¹,

Poulsen²,

Nielsen³

et al. 2012

IJN

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Advances in biomedical nanotechnology raise hopes in patient populations but may also raise questions regarding biodistribution and biocompatibility, especially during pregnancy. Special consideration must be given to the placenta as a biological barrier because a pregnant woman's exposure to nanoparticles could have significant effects on the fetus developing in the womb. Therefore, the purpose of this study is to optimize an in vitro model for characterizing the transport of nanoparticles across human placental trophoblast cells. The growth of BeWo (clone b30) human placental choriocarcinoma cells for nanoparticle transport studies was characterized in terms of optimized Transwell® insert type and pore size, the investigation of barrier properties by transmission electron microscopy, tight junction staining, transepithelial electrical resistance, and fluorescein sodium transport. Following the determination of nontoxic concentrations of fluorescent polystyrene nanoparticles, the cellular uptake and transport of 50 nm and 100 nm diameter particles was measured using the in vitro BeWo cell model. Particle size measurements, fluorescence readings, and confocal microscopy indicated both cellular uptake of the fluorescent polystyrene nanoparticles and the transcellular transport of these particles from the apical (maternal) to the basolateral (fetal) compartment. Over the course of 24 hours, the apparent permeability across BeWo cells grown on polycarbonate membranes (3.0 µm pore size) was four times higher for the 50 nm particles compared with the 100 nm particles. The BeWo cell line has been optimized and shown to be a valid in vitro model for studying the transplacental transport of nanoparticles. Fluorescent polystyrene nanoparticle transport was size-dependent, as smaller particles reached the basal (fetal) compartment at a higher rate

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Tissue distribution of 20nm, 100nm and 1000nm fluorescent polystyrene latex nanospheres following acute systemic or acute and repeat airway exposure in the rat

Cited by 76 publications

References 19 publications

Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping

Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping

Formulation and evaluation of gemcitabine-loaded solid lipid nanoparticles

In vitro placental model optimization for nanoparticle transport studies

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