Tissue Distribution of Human Pyruvate Kinase Isozymes

Rh, Bigley; Stenzel, Peter; Rt, Jones; Jo, Campos; Rd, Koler

doi:10.1159/000458230

Cited by 72 publications

(20 citation statements)

References 13 publications

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“…Also it has been reported by Bigley et al [16] that human tissues contain three isoenzymes of pyruvate kinase. From studies of the antigenic behaviour of the enzymes the authors suggest a dimeric structure for the three forms, one of them being an hybrid of the other two; nevertheless the three forms have never been found in the same tissue.…”

Section: Discussionmentioning

confidence: 89%

Multiple Molecular Forms of Pyruvate Kinase from Mucor rouxii

Friedenthal¹,

Roselino

Passeron³

1973

European Journal of Biochemistry

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Pyruvate kinase from Mucor rouxii constitutes a family of three isoenzymes which can be separated in DEAE-cellulose columns. These have been named type I, I1 and 111. They also have different electrophoretic mobility in polyacrylamide gels. Pyruvate kinases type I, I1 and I11were partially purified by ion-exchange chromatography. Antisera were prepared against types I and 111. The antigenic behaviour of the three isoenzymes supported the hypothesis on the hybrid nature of pyruvate kinase type I1 and confirmed the identity of pyruvate kinase from yeastlike cells and type I from mycelium.The filamentous form contains the three types, none of them being constitutive and the proportion of each depends on the concentration and on the nature of the carbohydrate in the growth medium. Under gluconeogenic conditions of growth type 111 predominantes while in any condition in which aerobic glycosis is favoured type I is the dominant form. We also show the results of a detailed study of the effects of nutritional factors on the enzymatic pattern of pyruvate kinases in M . rouxii.Evidence is presented indicating that under a variety of conditions the filamentous form contains the three forms of the enzyme, which can be distinguished from each other on DEAE-cellulose columns. None of the three forms seems to be constitutive, the relative proportions of each form vary according to the growing conditions as well as to the morphology of the fungus. Kinetics of induction of pyruvate kinase I1 and I11 during aerobic germination of spores under gluconeogenic conditions, accompanied by disappearance of type I and also reappearance of types I and I1 upon addition of glucose or xylose to growing cultures devoid

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Section: Discussionmentioning

confidence: 89%

Multiple Molecular Forms of Pyruvate Kinase from Mucor rouxii

Friedenthal¹,

Roselino

Passeron³

1973

European Journal of Biochemistry

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“…The conditions for electrophoresis and staining of MPI activity were conducted according to the method of N ichols and Ruddle (1973). Starch-gel electrophoresis for PK-3 was carried out in tris-borate buffer (0.18 M tris and 0.1 M borate in cathodal solution; 0.129 M tris and 0.071 M borate in anodal solution), pH 8.4, containing 2 him 2-mercaptoethanol and 0.1 dim fructose-l,6-diphosphate at 17.5 V/cm across the gel for 18 h. Staining for PK-3 activity was performed by two methods; (1) PK-3 activity was detected by coupling to the lactic dehydrogenase reaction by a modification of the method described by Bigley et al (1968). Briefly, after electrophoresis, the sliced gel was overlaid with filter paper saturated with the staining reaction mixture, consisting of 0.1 M tris-HCl (pH 8.0).…”

Section: Methodsmentioning

confidence: 99%

Confirmation of the synteny of the human genes for mannose phosphate isomerase and pyruvate kinase and of their assignment to chromosome 15

Chern¹,

Croce²

1975

Cytogenet Genome Res

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The synteny of human mannose phosphate isomerase and pyruvate kinase and the assignment of the genes for these two enzymes to chromosome 15 were confirmed by analysis of 43 independently derived human-mouse hybrid clones. Hybrids between mouse cells deficient in hypoxanthine-guanine phosphoribosyl-transferase and human fibroblasts carrying an X/15 chromosome translocation were also included in this study.

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“…The first study on human tissues was performed by Bigley et al [1] with starch gel electrophoresis. The authors separated isozyme I in liver and erythrocyte, isozyme II in kidney, and isozyme III in liver, kidney, leuko cytes, skeletal muscle and cardiac muscle.…”

Section: Introductionmentioning

confidence: 99%

Pyruvate Kinase Isozymes among Human Organs and Blood Cells

Etiemble¹,

Boivin²

1976

Enzyme

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In extracts of human tissues only three different pyruvate kinases were demonstrated by acetate gel electrophoresis. Treatment with reduced glutathione resulted in an alteration of electrophoretic mobility of extracts from kidney and testis. The existence of interconvertible forms of M(2) pyruvate kinase (PK) was suggested. Lymphocytes, polymorphonuclear cells, platelets were M(2) type PK. An electrophoretic slow form was demonstrated in myeloblasts and lymphoblasts.

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Tissue Distribution of Human Pyruvate Kinase Isozymes

Cited by 72 publications

References 13 publications

Multiple Molecular Forms of Pyruvate Kinase from Mucor rouxii

Multiple Molecular Forms of Pyruvate Kinase from Mucor rouxii

Confirmation of the synteny of the human genes for mannose phosphate isomerase and pyruvate kinase and of their assignment to chromosome 15

Pyruvate Kinase Isozymes among Human Organs and Blood Cells

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