Tissue-engineered artificial oesophagus patch using three-dimensionally printed polycaprolactone with mesenchymal stem cells: a preliminary report

Park, Sung Yong; Choi, Jae Won; Park, Ju-Kyeong; Song, Eun Hye; Park, Su A; Kim, Yeon Soo; Shin, Yoo Seob; Kim, Chul‐Ho

doi:10.1093/icvts/ivw048

Cited by 43 publications

(28 citation statements)

References 26 publications

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“…31,[37][38][39][40] When used in combination with acellular matrix for esophageal replacement strategies, MSCs were shown to promote re-epithelialization, to accelerate muscle colonization and regeneration, and to exert an anti-inflammatory role. [41][42][43][44] In this study, we describe a preliminary approach to the esophageal substitution based on a homologous decellularized scaffold 45,46 seeded with autologous bone-marrow-derived MSCs (BM-MSCs). In order to enhance cell seeding throughout the scaffold, this was microperforated by needles using Quantum Molecular Resonance ® (QMR).…”

Section: Introductionmentioning

confidence: 99%

Successful muscle regeneration by a homologous microperforated scaffold seeded with autologous mesenchymal stromal cells in a porcine esophageal substitution model

Marzaro

Algeri

Tomao

et al. 2020

Therap Adv Gastroenterol

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Background: Since the esophagus has no redundancy, congenital and acquired esophageal diseases often require esophageal substitution, with complicated surgery and intestinal or gastric transposition. Peri-and-post-operative complications are frequent, with major problems related to the food transit and reflux. During the last years tissue engineering products became an interesting therapeutic alternative for esophageal replacement, since they could mimic the organ structure and potentially help to restore the native functions and physiology. The use of acellular matrices pre-seeded with cells showed promising results for esophageal replacement approaches, but cell homing and adhesion to the scaffold remain an important issue and were investigated. Methods: A porcine esophageal substitute constituted of a decellularized scaffold seeded with autologous bone marrow-derived mesenchymal stromal cells (BM-MSCs) was developed. In order to improve cell seeding and distribution throughout the scaffolds, they were micro-perforated by Quantum Molecular Resonance (QMR) technology (Telea Electronic Engineering). Results: The treatment created a microporous network and cells were able to colonize both outer and inner layers of the scaffolds. Non seeded (NSS) and BM-MSCs seeded scaffolds (SS) were implanted on the thoracic esophagus of 4 and 8 pigs respectively, substituting only the muscle layer in a mucosal sparing technique. After 3 months from surgery, we observed an esophageal substenosis in 2/4 NSS pigs and in 6/8 SS pigs and a non-practicable stricture in 1/4 NSS pigs and 2/8 SS pigs. All the animals exhibited a normal weight increase, except one case in the SS group. Actin and desmin staining of the post-implant scaffolds evidenced the regeneration of a muscular layer from one anastomosis to another in the SS group but not in the NSS one. Conclusions: A muscle esophageal substitute starting from a porcine scaffold was developed and it was fully repopulated by BM-MSCs after seeding. The substitute was able to recapitulate in shape and function the original esophageal muscle layer.

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Section: Introductionmentioning

confidence: 99%

Successful muscle regeneration by a homologous microperforated scaffold seeded with autologous mesenchymal stromal cells in a porcine esophageal substitution model

Marzaro

Algeri

Tomao

et al. 2020

Therap Adv Gastroenterol

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“…Previous reports on regeneration of epithelial and muscular regions of esophagus in small and large animal models show the possibility of in vivo regeneration of partially recellularized natural or artificial scaffolds. [22][23][24] This tissue-specific differentiation of cell types in recellularized tissue suggest that engineered esophagus would be suitable for future in vivo applications.…”

Section: Discussionmentioning

confidence: 93%

In Vitro Regeneration of Decellularized Pig Esophagus Using Human Amniotic Stem Cells

Nayakawde

Methe

Banerjee

et al. 2020

BioResearch Open Access

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Decellularization of esophagus was studied using three different protocols. The sodium deoxycholate/DNase-I (SDC/DNase-I) method was the most successful as evidenced by histology and DNA quantification of the acellular scaffolds. Acellular scaffolds were further analyzed and compared with native tissue by histology, quantitative analysis of DNA, and extracellular matrix (ECM) proteins. Histologically, the SDC/DNase-I protocol effectively produced scaffold with preserved structural architecture similar to native tissue architecture devoid of any cell nucleus. ECM proteins, such as collagen, elastin, and glycosaminoglycans were present even after detergentenzymatic decellularization. Immunohistochemical analysis of acellular scaffold showed weak expression of Gal 1, 3 Gal epitope compared with native tissue. For performing recellularization, human amnion-derived mesenchymal stem cells (MSCs) and epithelial cells were seeded onto acellular esophagus in a perfusion-rotation bioreactor. In recellularized esophagus, immunohistochemistry showed infiltration of MSCs from adventitia into the muscularis externa and differentiation of MSCs into the smooth muscle actin and few endothelial cells (CD31). Our study demonstrates successful preparation and characterization of a decellularized esophagus with reduced load of Gal 1, 3 Gal epitope with preserved architecture and ECM proteins similar to native tissue. Upon subsequent recellularization, xenogeneic acellular esophagus also supported stem cell growth and partial differentiation of stem cells. Hence, the current study offers the hope for preparing a tissue-engineered esophagus in vitro which can be transplanted further into pigs for further in vivo evaluation.

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“…PCL is a synthetic, non‐toxic, absorbable polymer that can be widely applied in the field of tissue engineering. Porous PCL scaffolds have favorable mechanical properties and are resistant to rapid hydrolysis . Jang et al reported that PCL scaffolds used with human umbilical cord serum or beta‐tricalcium phosphate led to improved bone formation compared with controls.…”

Section: Discussionmentioning

confidence: 99%

“…Porous PCL scaffolds have favorable mechanical properties and are resistant to rapid hydrolysis. 25 Jang et al reported that PCL scaffolds used with human umbilical cord serum 26 or beta-tricalcium phosphate 27 led to improved bone formation compared with controls. Our micro-CT imaging and histological results also revealed a significant difference in bone formation between the groups with and without PCL scaffolds.…”

Section: Discussionmentioning

confidence: 99%

Osteogenesis for postoperative temporal bone defects using human ear adipose‐derived stromal cells and tissue engineering: An animal model study

Kim

Park

Shin

et al. 2017

J Biomedical Materials Res

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Mastoidectomy, the removal of infected mastoid bones, is a common surgical procedure for the treatment of chronic otitis media. Persistent and recurrent otorrhea and accumulation of keratin debris following open cavity mastoidectomy are still bothersome issues for both patients and otologists. In this study, we used human ear adipose-derived stromal cells (hEASCs) in combination with polycaprolactone (PCL) scaffolds and osteogenic differentiation medium (ODM) to regenerate temporal bone defects. The hEASCs showed stem cell phenotypes, and these characteristics were maintained up to passage 5. Mastoid bulla and cranial bone defects were induced in Sprague-Dawley rats using AgNO and burr hole drilling, respectively, and the rats were then divided into five groups: (1) control, (2) hEASCs, (3) hEASCs + ODM, (4) hEASCs + PCL scaffolds, and (5) hEASCs + PCL scaffolds + ODM. Osteogenesis was evaluated by micro-computed tomography and histology. Compared with the control group, the groups transplanted with hEASCs and PCL scaffolds had significantly higher bone formation along the periphery of the mastoid bulla area. Moreover, ODM synergistically enhanced bone formation in mastoid bulla defects. Our results suggest that combining hEASCs with PCL scaffolds represents a promising method for anatomical and functional reconstruction of postoperative temporal bone defects following mastoidectomy. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3493-3501, 2017.

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Tissue-engineered artificial oesophagus patch using three-dimensionally printed polycaprolactone with mesenchymal stem cells: a preliminary report

Abstract: Use of the 3DP scaffold coated with MSCs seeded in fibrin resulted in successful restoration of the shape and histology of the cervical oesophagus without any graft rejection; thus, this is a promising material for use as an artificial oesophagus.

Cited by 43 publications

References 26 publications

Successful muscle regeneration by a homologous microperforated scaffold seeded with autologous mesenchymal stromal cells in a porcine esophageal substitution model

Successful muscle regeneration by a homologous microperforated scaffold seeded with autologous mesenchymal stromal cells in a porcine esophageal substitution model

In Vitro Regeneration of Decellularized Pig Esophagus Using Human Amniotic Stem Cells

Osteogenesis for postoperative temporal bone defects using human ear adipose‐derived stromal cells and tissue engineering: An animal model study

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