Tissue engineering and stem cell therapy in pediatric urology

Sharma, Swati; Gupta, Devendra K.

doi:10.4103/jiaps.jiaps_77_18

Cited by 16 publications

(10 citation statements)

References 75 publications

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“…Gastrointestinal segments are frequently used in orthotopic neobladder reconstruction and bladder augmentation. However, gastrointestinal segments have got the capability of absorption of diverse specific substances [11,12]. Contrastingly, neobladder tissues are composed of specialized cells for storage and disposal of the solutes.…”

Section: Discussionmentioning

confidence: 99%

The constitution of functional model rabbit bladder with acellular matrix

Altıok¹,

Özbay²,

Ekmekçioğlu³

2021

J CLIN MED KAZ

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In this study, for the useable of bladder acelluar matrix in urinary tissue engineering, we compared the urodynamic and histopathological results between normal, partial cystectomy and bladder acellular matrix allograft (BAMA) augmented cystoplasty groups in rabbit model.Material and methods: There were five groups each consisting of three rabbits. Urodynamic investigation was applied to all animals at the beginning of the study. Then, in the first control group, the rabbits were sacrified and the bladders were removed for histopathological examination. In the second control group the same procedures were realized at the end of 24th week. In the third group, partial cystectomy was performed to the animals and followed for 24 weeks. Partial cystectomy and BAMA augmented cystoplasty were performed to the animals in the 4th and 5th groups. The animals were sacrified at the end of 12th and 24th weeks, respectively. In the 3th, 4th and 5th groups urodynamic investigations were performed before sacrification. Then bladders underwent histopathological examination.Results: There was no significant difference in term of bladder capacity between both control and BAMA augmented groups. Detrusor presures were no statistical different in all groups. However bladder capacity has significantly reduced in partial cystectomy group. In histopathological evaluations there were no differences between control and partial cystectomy groups. In BAMA augmented groups urothelium and vascularization were nearly the same as control groups however muscle layer was less organized and was thinner than control groups. Collagen depositon and innervation in BAMA augmented bladder were more prominent than that of the control group. Conclusion:In our animal study, although the detrusor muscle had less organized with more collagen deposition, the BAMA augmented bladders had near normal capacity and compliance with normal detrusor presures.

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Section: Discussionmentioning

confidence: 99%

The constitution of functional model rabbit bladder with acellular matrix

Altıok¹,

Özbay²,

Ekmekçioğlu³

2021

J CLIN MED KAZ

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“…The use of ESCs is controversial due to ethical issues but also to their high capacity to create teratomas after injection. Interestingly, adult SCs can be harvested from many tissues (Figure 2 ), such as the brain, bone marrow (BM), peripheral blood, blood vessels, skeletal muscle, skin, cornea, retina, tooth dental pulp, digestive system, liver and, pancreas[ 47 ]. They can be divided in two main categories.…”

Section: Regenerative Medicine Strategies Using Scsmentioning

confidence: 99%

Considerations for the clinical use of stem cells in genitourinary regenerative medicine

Caneparo

Sorroza-Martinez

Chabaud

et al. 2021

WJSC

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The genitourinary tract can be affected by several pathologies which require repair or replacement to recover biological functions. Current therapeutic strategies are challenged by a growing shortage of adequate tissues. Therefore, new options must be considered for the treatment of patients, with the use of stem cells (SCs) being attractive. Two different strategies can be derived from stem cell use: Cell therapy and tissue therapy, mainly through tissue engineering. The recent advances using these approaches are described in this review, with a focus on stromal/mesenchymal cells found in adipose tissue. Indeed, the accessibility, high yield at harvest as well as anti-fibrotic, immunomodulatory and proangiogenic properties make adipose-derived stromal/SCs promising alternatives to the therapies currently offered to patients. Finally, an innovative technique allowing tissue reconstruction without exogenous material, the self-assembly approach, will be presented. Despite advances, more studies are needed to translate such approaches from the bench to clinics in urology. For the 21 st century, cell and tissue therapies based on SCs are certainly the future of genitourinary regenerative medicine.

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“…There is also a tremendous need for kidney, bladder and urethra regeneration therapies in children with congenital anomalies. Although there are positive experimental data from animal models, and some promising results of early clinical trials of bladder regeneration using a combination of stem cells and biomaterials in children with neurogenic bladder, which is a neurologic defect associated with spina bifida, so far there are no effective stem cell therapies in paediatric urology (10,44,45) .…”

Section: Therapeutic Application Of Mscs In Paediatric Diseasesmentioning

confidence: 99%

“…Induced pluripotent stem cells (iPSCs) are artificially created in the laboratory through the reprogramming of differentiated cells into embryonic-like stem cells (5)(6)(7) . The main sources of stem cells used in paediatric therapies are umbilical cord and umbilical cord blood, amniotic fluid, placenta, bone marrow, adipose tissue, urine, and iPSCs derived from the patient's cells (8)(9)(10) . Some of these findings have led to the development of cord blood banks that store umbilical cord blood which can be a source of haematopoietic cells for various therapies (11) and new models for the therapeutic testing of paediatric cancers (12) .…”

mentioning

confidence: 99%

Double face of stem cells in paediatrics: therapeutic applications of mesenchymal stem cells and threats from cancer stem cells

Uosef¹,

Ghobrial²,

Jęderka³

et al. 2020

Pediatr Med Rodz

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Decades of research have shown that many, if not all, fully developed and differentiated organs and tissues contain a subpopulation of undifferentiated stem cells or progenitors of stem cells, which under natural or experimental conditions can self-renew and differentiate into specialised cells. These findings have opened countless possibilities of novel therapeutic applications for the treatment of adult and child diseases. The main sources of stem cells used in paediatric therapies are umbilical cord and umbilical cord blood, amniotic fluid, placenta, bone marrow, adipose tissue, urine, and induced pluripotent stem cells derived from the patient's cells. Here, we describe some of the paediatrically applicable stem cell therapies. We focus our attention on the therapeutic applications of mesenchymal stem cells in paediatric diseases. An important but negative effect of stem cell therapies is the risk of oncogenic potential of therapeutically applied stem cells. Under certain circumstances, these stem cells can lead to tumour development. In addition, the majority of adult and paediatric tumours contain a subpopulation of cancer stem cells which are privileged therapeutic targets for numerous paediatric cancers. In this article, we review these two opposite properties ("double face") of stem cells in general and paediatric medicine.

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Tissue engineering and stem cell therapy in pediatric urology

Cited by 16 publications

References 75 publications

The constitution of functional model rabbit bladder with acellular matrix

The constitution of functional model rabbit bladder with acellular matrix

Considerations for the clinical use of stem cells in genitourinary regenerative medicine

Double face of stem cells in paediatrics: therapeutic applications of mesenchymal stem cells and threats from cancer stem cells

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