Tissue Engineering: New Tools for Old Problems

Pirraco, Rogério P.; Reis, Rui L.

doi:10.1007/s12015-015-9593-9

Cited by 7 publications

(5 citation statements)

References 28 publications

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“…Unfortunately, the demand largely overcomes the availability, as just 31,768 organs were received in the same year, causing the daily death of around 20 people waiting for an organ [1]. Tissue Engineering and Regenerative Medicine (TERM) strategies are seen as promising approaches to solve the issue of organ shortage [2]. However, limitations of traditional TERM strategies such as low anchorage to the desired site in the case of cell injection [3], strong host reaction in response to the biodegradation of the scaffolds [4], or insufficient delivery of oxygen and nutrients to the bulk of scaffolds, are precluding their widespread clinical application.…”

Section: Introductionmentioning

confidence: 99%

Strategies for the hypothermic preservation of cell sheets of human adipose stem cells

et al. 2019

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Cell Sheet (CS) Engineering is a regenerative medicine strategy proposed for the treatment of injured or diseased organs and tissues. In fact, several clinical trials are underway using CS-based methodologies. However, the clinical application of such cell-based methodologies poses several challenges related with the preservation of CS structure and function from the fabrication site to the bedside. Pausing cells at hypothermic temperatures has been suggested as a valuable method for short-term cell preservation. In this study, we tested the efficiency of two preservation strategies, one using culture medium supplementation with Rokepie and the other using the preservation solution Hypothermosol, in preserving human adipose stromal/stem cells (hASC) CS-like confluent cultures at 4°C, during 3 and 7 days. Both preservation strategies demonstrated excellent ability to preserve cell function during the first 3 days in hypothermia, as demonstrated by metabolic activity results and assessment of extracellular matrix integrity and differentiation potential. At the end of the 7th day of hypothermic incubation, the decrease in cell metabolic activity was more evident for all conditions. Nonetheless, hASC incubated with Rokepie and Hypothermosol retained a higher metabolic activity and extracellular matrix integrity in comparison with unsupplemented cells. Differentiation results for the later time point showed that supplementation with both Rokepie and Hypothermosol rescued adipogenic differentiation potential but only Rokepie was able to preserve hASC osteogenic potential.

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Section: Introductionmentioning

confidence: 99%

Strategies for the hypothermic preservation of cell sheets of human adipose stem cells

et al. 2019

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“…Heart transplantation procedures represented an expense of approximately 134 million euros in 2019 ( 2 ), while donation and transplantation of blood, tissues, and cells are estimated to represent around 6 billion euros per year in the EU alone ( 3 ). Tissue engineering and regenerative medicine (TERM) represent potential solutions to this problem and have therefore been attracting increased interest from clinicians ( 4 ). Tissue engineering approaches, in particular, typically use bioartificial 3D matrices termed scaffolds, composed of the most diverse materials, where cells are seeded and allowed to grow, to yield the desired tissue-like constructs.…”

Section: Introductionmentioning

confidence: 99%

Long-term and short-term preservation strategies for tissue engineering and regenerative medicine products: state of the art and emerging trends

2022

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There is an ever-growing need of human tissues and organs for transplantation. However, the availability of such tissues and organs is insufficient by a large margin, which is a huge medical and societal problem. Tissue engineering and regenerative medicine (TERM) represent potential solutions to this issue and have therefore been attracting increased interest from researchers and clinicians alike. But the successful large-scale clinical deployment of TERM products critically depends on the development of efficient preservation methodologies. The existing preservation approaches such as slow freezing, vitrification, dry state preservation, and hypothermic and normothermic storage all have issues that somehow limit the biomedical applications of TERM products. In this review, the principles and application of these approaches will be summarized, highlighting their advantages and limitations in the context of TERM products preservation.

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“…Previous studies have revealed that as a major component of multicellular organisms, extracellular matrix (ECM) not only provides necessary mechanical and adhesive support to the cells, but also regulates various cell behaviors through facilitating the transmission of biochemical cues [1][2][3][4][5][6]. Systematic investigations on the interactions between the cell behaviors and its ECM can not only improve our understandings on the mechanisms underlying intricate cellular activities, but also contribute to the development of tissue engineering strategies for effective cell therapies [7][8][9][10][11][12][13]. Despite the vast advancement made in in-vivo studies, due to the complex and dynamic nature of physiological environment, in-vitro approaches are still widely applied in exploring some complex interplay between cells and their ECM, where cells are cultured and perturbed in bio-mimic scaffolds that are highly recapitulating their physiological microenvironment yet remain great flexibility in manipulatable components to decouple the entangled factors.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of cell-laden alginate microgels with tunable compositions based on microfluidic pico-injection technique

Zhang¹,

Mao

Mooney

et al. 2022

Preprint

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We report a microfluidic pico-injection-based approach for reliably generating monodisperse cell-laden alginate microgels whose composition can be tuned in situ through modulation of the cross-linker concentration. Separating the gelation from emulsification allows for a better control over the microgel size with a microfluidic drop-maker, and an instant adjustment of the microgel composition with a pico-injector.

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Tissue Engineering: New Tools for Old Problems

Cited by 7 publications

References 28 publications

Strategies for the hypothermic preservation of cell sheets of human adipose stem cells

Strategies for the hypothermic preservation of cell sheets of human adipose stem cells

Long-term and short-term preservation strategies for tissue engineering and regenerative medicine products: state of the art and emerging trends

Synthesis of cell-laden alginate microgels with tunable compositions based on microfluidic pico-injection technique

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