Tissue engineering of skin

Böttcher‐Haberzeth, Sophie; Biedermann, Thomas; Ernst, Rolf

doi:10.1016/j.burns.2009.08.016

Cited by 327 publications

(214 citation statements)

References 115 publications

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“…They contain cells of different origin (autologous, allogeneic or xenogeneic) and biodegradable materials (naturally occurring or synthetic polymers) as scaffolds for cell attachment and facilitated handling. For skin tissue engineering, the challenge that remains is the generation of a complex dermis-epidermal substitute that can be securely and conveniently transplanted with minimal scarring in one single surgical intervention 2 .…”

Section: Introductionmentioning

confidence: 99%

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

2015

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Chitosan is soluble in diluted acid solutions and can easily form films by casting. However, residual acid neutralization should be performed for biomedical applications what may compromise physical and mechanical properties of the films. Thus, plasticizers can be added to improve these properties. The aim of this study was to characterize morphological, barrier and mechanical properties, besides evaluate the in vitro cytotoxicity of sorbitol-plasticized and NaOH-Na 2 CO 3 neutralized chitosan membranes for skin substitute application. Scanning electron microscopy, X-ray diffraction, water vapor permeability and mechanical tests were carried out to characterize the obtained membranes. Moreover, Vero cells were used for in vitro cytotoxicity evaluation. In this paper, we report a non-cytotoxic sorbitol-plasticized chitosan membrane with desirable properties for skin substitution, such as flexibility, water vapor permeability and high percentage of elongation.

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Section: Introductionmentioning

confidence: 99%

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

2015

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“…Funcionalmente, su principal alcance ha sido proporcionar una cobertura al lecho de la lesión, evitando la deshidratación y las infecciones. Estos productos contienen células de diversos orígenes (autólogo, alogénico o xenogénico) y materiales biodegradables (naturales o polímeros sintéticos) que sirven como matrices para la adhesión y proliferación celular, al mismo tiempo que facilitan la manipulación para la aplicación terapéutica (Böttcher-Haberzeth et al, 2010).…”

Section: Tejidos Cultivadosunclassified

“…En este sentido, los retos actuales incluyen la elaboración de tejidos de larga duración y adecuada vascularización (Böttcher-Haberzeth et al, 2010).…”

Section: Introductionunclassified

Revista Tecnología en Marcha. Número Especial Ingeniería de Tejidos. Mayo 2015

Celular¹

2015

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“…To this two-component system, bioactive agents that accelerate growth and increase differentiation of cells are added to hasten tissue formation. An ideal scaffold should have appropriate physical and chemical structure and surface properties to allow cell attachment, proliferation and differentiation as well as physiologically correct cellular behaviors [7,8].…”

Section: Introductionmentioning

confidence: 99%

Electrospinning of nanofibrous polycaprolactone (PCL) and collagen-blended polycaprolactone for wound dressing and tissue engineering

Zeybek¹,

Duman²,

Ürkmez³

2014

uujms

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Fabrication of nanofibrous biomaterials based on natural materials through various techniques is a popular research topic, particularly for biomedical applications. Electrospinning, a well-established technique for nanofiber production has also been extended for producing nanofibrous structures of natural materials that mimic natural extracellular matrix of mammalian tissues. Collagen nanofiber production utilizes hexafluoro propanol (HFP) as a solvent for electrospinning. A novel cost-effective electrospun nanofibrous membrane is established for wound dressing and allogeneic cultured epidermal substitute through the cultivation of human dermal keratinocytes for skin defects. Several synthetic polymers such as polycaprolactone (PCL) are generally electrospun for tissue engineering applications because of their remarkable mechanical stability and slow degradation rates. The large surface area of the polymer nanofibers with specific modifications facilitates cell adhesion and control of their cellular functions. The objectives of this study were to optimize fabrication parameters of electrospun nanofibrous membranes from biodegradable PCL and collagen-blended nanofibrous membranes to combine mechanical integrity and spinnability of PCL with high biocompatibility of collagen, and to examine keratinocyte attachment, morphology, proliferation, and cell-matrix interactions. Results prove that the porous nanofibrous PCL and modified PCL-blended collagen nanofibrous membranes are suitable for the attachment and proliferation of keratinocytes, and might have the potential to be applied as wound dressing as well as in tissue engineering as an epidermal substitute for the treatment of skin defects and burn wounds.

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Tissue engineering of skin

Cited by 327 publications

References 115 publications

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

Revista Tecnología en Marcha. Número Especial Ingeniería de Tejidos. Mayo 2015

Electrospinning of nanofibrous polycaprolactone (PCL) and collagen-blended polycaprolactone for wound dressing and tissue engineering

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