Tissue engineering of urinary bladder using acellular matrix

Glybochko, Petr; Olefir, Yu. V.; Alyaev, Yu G; Butnaru, Denis; Безруков, Е. А.; Chaplenko, A. A.; Zharikova, Tatiana M.

doi:10.18565/urol.2017.1.89-94

Cited by 4 publications

(4 citation statements)

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“…We did not observe transitional cells on the new wall in any of the groups. We believe that this outcome was probably due to the limited survival time according to other studies [ 8 , 24 , 25 ]. We want to emphasize that in bladder regeneration, not only is the presence of urothelial cells important, but also the muscle fiber regeneration, which we did not properly observe in any of the groups.…”

Section: Discussionmentioning

confidence: 81%

“…This matrix acts as a scaffold to promote bladder regeneration, it is biodegradable and easy to handle, and does not induce an immune response. In general, it has better results in urothelial epithelialization rather than in muscle fiber regeneration, therefore it can finally affect the functional outcomes [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies on animals and humans showed good results in terms of urothelial re-epithelialization, but with an inadequate muscle regeneration because it was incomplete, disorganized, not well integrated into the new wall, and without adequate vascularization or innervation [ 7 ]. This fact determines the final bladder functionality [ 24 , 26 ]. Some studies have even performed a specific mechanical analysis to study the tensile strength of the new wall [ 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

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Use of an acellular collagen–elastin matrix to support bladder regeneration in a porcine model of peritoneocystoplasty

2018

Cent european J Uro

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IntroductionBladder reconstruction without using the intestine remains a challenge to this day despite the development of new biomaterials and cell cultures. Human bladder engineering is merely anecdotic, and mostly in vitro and animal studies have been conducted.Material and methodsIn our study using a porcine model, we performed a bladder augmentation using an autologous parietal peritoneum graft (peritoneocystoplasty) and determined whether the attachment of an acellular collagen-elastin matrix (Group 1) or lack of (Group 2) had better histologic and functional results. Thus far, peritoneocystoplasty has rarely been performed or combined with a biomaterial.ResultsAfter 6 weeks, we observed different degrees of retraction of the new bladder wall in both groups, although the retraction was lower and the histological analysis showed more signs of regeneration (neoangiogenesis and less fibrosis) in Group 1 than when compared with Group 2. No transitional cells were found in the new bladder wall in any of the groups, and no differences were observed in the functional test results.ConclusionsPerforming a peritoneocystoplasty is an easy and safe procedure. The data supports the benefit of an acellular collagen–elastin matrix to reinforce bladder regeneration. However, in our study we observed too much retraction of the new wall and the histologic results were not acceptable to consider it an appropriate cystoplasty technique.

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Use of an acellular collagen–elastin matrix to support bladder regeneration in a porcine model of peritoneocystoplasty

2018

Cent european J Uro

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“…No tissue rejection potential and detection of almost normal bladder capacity and compliance levels were the positive aspects of the neobladders created by use of acellular matrix technique. Only the growth of the muscle layer was observed inadequeately [21]. Yoyo et al seeded BAM grafts with urothelial and smooth muscle cells for augmentation of the partial cystectomy in a canine model.…”

Section: Discussionmentioning

confidence: 99%

The constitution of functional model rabbit bladder with acellular matrix

Altıok¹,

Özbay²,

Ekmekçioğlu³

2021

J CLIN MED KAZ

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In this study, for the useable of bladder acelluar matrix in urinary tissue engineering, we compared the urodynamic and histopathological results between normal, partial cystectomy and bladder acellular matrix allograft (BAMA) augmented cystoplasty groups in rabbit model.Material and methods: There were five groups each consisting of three rabbits. Urodynamic investigation was applied to all animals at the beginning of the study. Then, in the first control group, the rabbits were sacrified and the bladders were removed for histopathological examination. In the second control group the same procedures were realized at the end of 24th week. In the third group, partial cystectomy was performed to the animals and followed for 24 weeks. Partial cystectomy and BAMA augmented cystoplasty were performed to the animals in the 4th and 5th groups. The animals were sacrified at the end of 12th and 24th weeks, respectively. In the 3th, 4th and 5th groups urodynamic investigations were performed before sacrification. Then bladders underwent histopathological examination.Results: There was no significant difference in term of bladder capacity between both control and BAMA augmented groups. Detrusor presures were no statistical different in all groups. However bladder capacity has significantly reduced in partial cystectomy group. In histopathological evaluations there were no differences between control and partial cystectomy groups. In BAMA augmented groups urothelium and vascularization were nearly the same as control groups however muscle layer was less organized and was thinner than control groups. Collagen depositon and innervation in BAMA augmented bladder were more prominent than that of the control group. Conclusion:In our animal study, although the detrusor muscle had less organized with more collagen deposition, the BAMA augmented bladders had near normal capacity and compliance with normal detrusor presures.

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