Tissue-specific induction of EROD activity and CYP1A protein in Sparus aurata exposed to B(a)P and TCDD

Ortiz-Delgado, Juan B.; Behrens, Anja; Segner, Helmut; Sarasquete, Carmen

doi:10.1016/j.ecoenv.2006.12.021

Cited by 59 publications

(24 citation statements)

References 27 publications

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“…Andersson et al, 2007;Ortiz-Delgado et al, 2009). The difference in absolute levels between liver and gills are related to the amount of CYP1A proteins in these tissues.…”

Section: Resultsmentioning

confidence: 98%

EROD activity in liver and gills of polar cod (Boreogadus saida) exposed to waterborne and dietary crude oil

Nahrgang

Jönsson

Camus

2010

Marine Environmental Research

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“…Andersson et al, 2007;Ortiz-Delgado et al, 2009). The difference in absolute levels between liver and gills are related to the amount of CYP1A proteins in these tissues.…”

Section: Resultsmentioning

confidence: 98%

EROD activity in liver and gills of polar cod (Boreogadus saida) exposed to waterborne and dietary crude oil

Nahrgang

Jönsson

Camus

2010

Marine Environmental Research

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“…Although Cyp1a is the main enzyme catalyzing the de-ethylation of ethoxyresorufin, other CYP enzymes also can contribute to EROD activity (Spearow et al 2011). It has also repeatedly been reported that EROD can substantially be inhibited by elevated levels of PCBs, fluoranthene (a prevalent PAH), dioxins and heavy metals (e.g., Viarengo et al 1997;Gooch et al 1989;Willett et al 2001;Ortiz-Delgado et al 2008). EROD activity is technically easy to measure, and it is still considered as a robust biomarker (Oris and Roberts 2007); however, this enzymatic activity biomarker shows some limitations that can make interpretation sometimes difficult in field studies (Spearow et al 2011).…”

Section: Discussionmentioning

confidence: 96%

“…Rees et al 2003;Chung-Davidson et al 2004;Aluru and Vijayan 2008) and protein content or EROD activity (e.g. Gravato and Santos 2002;Miller et al 2004;Ortiz-Delgado et al 2008), relatively few have compared gene expression and protein level Hahn and Stegeman 1994). In this study, we focus on both Cyp1a induction through gene expression and protein content with state of the art techniques in order to provide a combined biomarker applicable for Morone saxatilis.…”

Section: Discussionmentioning

confidence: 97%

Cytochrome P4501A mRNA and protein induction in striped bass (Morone saxatilis)

Durieux

Connon

Werner

et al. 2012

Fish Physiol Biochem

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The striped bass (Morone saxatilis) supports a valuable recreational fishery and is among the most important piscivorous fish of the San Francisco Estuary. This species has suffered a significant decline in numbers over the past decades, and there is indication that contaminants are important contributors. Polycyclic aromatic hydrocarbons (PAHs) and polyhalogenated aromatic hydrocarbons (PHAHs) including PCBs and dioxins are widespread in the estuary, they typically bioaccumulate through trophic levels, reaching highest levels in top predators and are known to affect the fish health and development. The aim of this study was to investigate the dynamics of cytochrome P4501A (Cyp1a) induction simultaneously at different levels of biological organization (RNA transcription and protein synthesis) as a biomarker of exposure to PAHs and PHAHs. We utilized β-naphthoflavone (BNF) as a model PAH to induce Cyp1a responses in juvenile striped bass in both dose-response and time-response assessments and determined Cyp1a mRNA and protein levels. Significant responses were measured in both systems at 10 mg ΒΝF kg⁻¹, a concentration used for time-response studies. Messenger RNA levels peaked at 6 h post-injection, while protein levels increased progressively with time, significantly peaking at 96 h post-injection; both remaining elevated throughout the duration of the test (8 days). Our data suggest that rapid induction of gene transcription following exposure and subsequent cumulative protein synthesis could provide a useful means of identifying temporal variants in exposure to Cyp1a inducers in Morone saxatilis. The potential application of this combined Cyp1a gene and protein biomarker in this species for field studies is discussed.

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“…Other studies have demonstrated that increasing levels of PAH contaminants in sediments, diet and water result in higher levels of FACs in fish exposed to these contaminants (Ortiz-Delgado et al, 2008;Vieira et al, 2009).…”

Section: Liver Erod Activitymentioning

confidence: 98%

“…There are numerous previous studies on EROD activity in liver but few studies have been conducted in gills (Mdegela et al, 2006;Ortiz-Delgado et al, 2008;Nahrgang et al, 2010) and only a few of them in relation with heavy metals (Mondon et al, 2001;Jönsson et al, 2004).…”

Section: Gill Erod Activitymentioning

confidence: 99%

EROD activity and cytochrome P4501A induction in liver and gills of Senegal sole Solea senegalensis from a polluted Huelva Estuary (SW Spain)

Oliva

Gravato

Guilhermino

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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EROD activity and induction cytochrome P4501A in liver and gills of Senegal sole, Solea senegalensis, from a heavy metal and PAH polluted estuary, was studied. Liver and gill CYP1A catalytic activity was assessed at the enzyme activity level-measured as 7-ethoxyresorufin-O-deethylase and cellular localization of CYP1A in the liver was studied using immunohistochemistry. Liver EROD was correlated with phenanthrene-type metabolites in liver and copper concentrations in water. Strong CYP1A occurrence was observed in acinar pancreatic cells, pancreatic duct epithelium and vascular system endothelium and negative/rare induction were observed in hepatocytes and sinusoidal endothelium. In gills, EROD activity showed a significant correlation with different fractions of heavy metals in sediment but no correlation was observed between EROD activity and PAHs. Strongly positive CYP1A associated staining of the vascular system endothelia and primary filament cells and a moderate staining of pillar cells in gills were observed. The results substantiated the utility of EROD activity and CYP1A induction measurement as biomarkers for use by aquatic toxicologists and indicate that catalytic assays and immunohistochemical assays appear to be sensitive to different kinds of pollutants being the use of both methods recommended for monitoring programs.

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Tissue-specific induction of EROD activity and CYP1A protein in Sparus aurata exposed to B(a)P and TCDD

Cited by 59 publications

References 27 publications

EROD activity in liver and gills of polar cod (Boreogadus saida) exposed to waterborne and dietary crude oil

EROD activity in liver and gills of polar cod (Boreogadus saida) exposed to waterborne and dietary crude oil

Cytochrome P4501A mRNA and protein induction in striped bass (Morone saxatilis)

EROD activity and cytochrome P4501A induction in liver and gills of Senegal sole Solea senegalensis from a polluted Huelva Estuary (SW Spain)

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