Tissue-specific metabolites profiling and quantitative analyses of flavonoids in the rhizome of Belamcanda chinensis by combining laser-microdissection with UHPLC-Q/TOF-MS and UHPLC–QqQ-MS

Chen, Yu Jie; Liang, Zhitao; Yu, Zhu; Xie, Guo; Tian, Mei; Zhao, Zhuo; Qin, Meng

doi:10.1016/j.talanta.2014.07.004

Cited by 30 publications

(11 citation statements)

References 34 publications

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“…This makes the border of each tissue more clear, enabling tissue or cell separation to be more accurate and specific [ 20 ]. The application of LMD and the UPLC-QqQ-MS/MS technique is proving to be a valuable technical platform for tissue- and cell-specific metabolite profiling of CMMs [ 21 ]. The method combining LMD and UPLC-QqQ-MS/MS can not only quantitatively analyze bioactive components of CMMs, but can also map the distribution patterns of bioactive components.…”

Section: Introductionmentioning

confidence: 99%

Tissue-Specific Analysis of Secondary Metabolites Creates a Reliable Morphological Criterion for Quality Grading of Polygoni Multiflori Radix

Liang

et al. 2018

Molecules

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In commercial herbal markets, Polygoni Multiflori Radix (PMR, the tuberous roots of Polygonum multiflorum Thunb.), a commonly-used Chinese medicinal material, is divided into different grades based on morphological features of size and weight. While more weight and larger size command a higher price, there is no scientific data confirming that the more expensive roots are in fact of better quality. To assess the inherent quality of various grades and of various tissues in PMR and to find reliable morphological indicators of quality, a method combining laser microdissection (LMD) and ultra-performance liquid chromatography triple-quadrupole mass spectrometry (UPLC-QqQ-MS/MS) was applied. Twelve major chemical components were quantitatively determined in both whole material and different tissues of PMR. Determination of the whole material revealed that traditional commercial grades based on size and weight of PRM did not correspond to any significant differences in chemical content. Instead, tissue-specific analysis indicated that the morphological features could be linked with quality in a new way. That is, PMR with broader cork and phloem, as seen in a transverse section, were typically of better quality as these parts are where the bioactive components accumulate. The tissue-specific analysis of secondary metabolites creates a reliable morphological criterion for quality grading of PMR.

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Section: Introductionmentioning

confidence: 99%

Tissue-Specific Analysis of Secondary Metabolites Creates a Reliable Morphological Criterion for Quality Grading of Polygoni Multiflori Radix

Liang

et al. 2018

Molecules

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“…As a famous alternative therapeutic herb, E. purpurea is usually considered to be efficient and natural with low toxic and fewer side effects. It was also mixed in the fodder as food supplements in animal husbandry (Chen et al, 2014). The major ingredients of E. purpurea are choric acid, caffeic acid, and other polyphenols and glycoproteins.…”

Section: Introductionmentioning

confidence: 99%

Echinacea purpurea Extract Affects the Immune System, Global Metabolome, and Gut Microbiome in Wistar Rats

Wang¹,

Hou²,

Lv³

et al. 2017

JAS

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Echinacea purpurea extract, a traditional herbal food additive with dual-purpose of medicine and edible material, has been widely used for the treatment and prevention of various infectious diseases, especially for children, old aged and immunocompromised patients. Although there were numerous reports suggested E. purpurea possessed immunostimulatory and antibacterial effects in vitro, the mechanisms underlying remained to be elucidated. This study employed immunologic factors analysis, GC-TOFMS based metabolomics and 16S-rRNA-sequencing microbiome profiling technologies to explore the effects of E. purpurea on young rats, a physiological insufficient immunity status, by compared with pidotimod treatment on young rats and adult animals. E. purpurea treatment significantly increased IL-2, decreased IL-6 and affect immunoglobulins in the spleen of young rats, indicating its promotion of cellular immunity. Both the immunologic factors and the global metabolome of E. purpurea treated young rats were close to the status of mature individuals. Results of 16S-rRNA-sequencing of ileum content together with co-metabolism metabolites demonstrated that E. purpurea changed gut microbiota structure characteristically as a reducing Firmicutes phylum, especially Lactobacillus, and a rising Actinobacteria phylum including Bifidobacterium. The results were concluded that E. purpurea could potentially promote the maturation of immune and metabolism of immature rats, and also affect gut flora structure.

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“…[12][13][14][15] Nuclear magnetic resonance (NMR) spectroscopy, matrix-assisted laser desorption/ionization mass spectrometry and fluorescence detection have all been used for the analysis of metabolites in tissues or single cells, [16][17][18][19][20] and the technique of laser microdissection (LMD) with fluorescence detection combined with liquid chromatography and tandem mass spectrometry (LC/MS/MS) has been shown to be especially suitable for tissue-specific metabolite profiling in situ. [21][22][23] Macleaya cordata from the Papaveraceae family with antibacterial, anti-inflammatory and anticancer properties has been used as a Traditional Chinese Medicine for over 1000 years. [24][25][26][27] Its major bioactive substances belong to the BIAs.…”

mentioning

confidence: 99%

Tissue‐specific metabolite profiling of benzylisoquinoline alkaloids in the root of Macleaya cordata by combining laser microdissection with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Zuo

Zheng

Liang

et al. 2017

Rapid Comm Mass Spectrometry

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The integrated method is sensitive, specific and reliable for determining trace alkaloids, which is also a powerful tool for metabolite profiling of tissue-specific BIAs in situ. The present findings should contribute to a better understanding of the biosynthesis of BIAs in M. cordata root and provide scientific evidence for its quality evaluation based on morphological characteristics. Copyright © 2016 John Wiley & Sons, Ltd.

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Tissue-specific metabolites profiling and quantitative analyses of flavonoids in the rhizome of Belamcanda chinensis by combining laser-microdissection with UHPLC-Q/TOF-MS and UHPLC–QqQ-MS

Cited by 30 publications

References 34 publications

Tissue-Specific Analysis of Secondary Metabolites Creates a Reliable Morphological Criterion for Quality Grading of Polygoni Multiflori Radix

Tissue-Specific Analysis of Secondary Metabolites Creates a Reliable Morphological Criterion for Quality Grading of Polygoni Multiflori Radix

Echinacea purpurea Extract Affects the Immune System, Global Metabolome, and Gut Microbiome in Wistar Rats

Tissue‐specific metabolite profiling of benzylisoquinoline alkaloids in the root of Macleaya cordata by combining laser microdissection with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

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