Tissue-specific transcriptome analyses reveal candidate genes for stilbene, flavonoid and anthraquinone biosynthesis in the medicinal plant Polygonum cuspidatum

Wang, Xiaowei; Hu, He-xuan; Wu, Zhijun; Fan, Haili; Wang, Guowei; Chai, Tuanyao; Wang, Hong

doi:10.1186/s12864-021-07658-3

Cited by 24 publications

(23 citation statements)

References 48 publications

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“…To extend the application field, we suggested the inclusion of medicinal orphan crops that also represent a secondary metabolite gold mine that deserves to be investigated. Among those orphan crops, the transcriptional comprehension of certain secondary metabolite synthesis has been initiated ( Kang et al, 2020 ; Hu et al, 2021 ; Wang et al, 2021a , b ) paving the way for not only effective JA triggered health-oriented metabolic engineering but also engineered crop protection against adverse biotic and abiotic stresses. Under the ongoing environmental change status, JAs’ mediated metabolite elicitation machinery needs further investigation in diverse plant systems.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview

et al. 2022

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Secondary metabolites are incontestably key specialized molecules with proven health-promoting effects on human beings. Naturally synthesized secondary metabolites are considered an important source of pharmaceuticals, food additives, cosmetics, flavors, etc., Therefore, enhancing the biosynthesis of these relevant metabolites by maintaining natural authenticity is getting more attention. The application of exogenous jasmonates (JAs) is well recognized for its ability to trigger plant growth and development. JAs have a large spectrum of action that covers seed germination, hypocotyl growth regulation, root elongation, petal expansion, and apical hook growth. This hormone is considered as one of the key regulators of the plant’s growth and development when the plant is under biotic or abiotic stress. The JAs regulate signal transduction through cross-talking with other genes in plants and thereby deploy an appropriate metabolism in the normal or stressed conditions. It has also been found to be an effective chemical elicitor for the synthesis of naturally occurring secondary metabolites. This review discusses the significance of JAs in the growth and development of plants and the successful outcomes of jasmonate-driven elicitation of secondary metabolites including flavonoids, anthraquinones, anthocyanin, xanthonoid, and more from various plant species. However, as the enhancement of these metabolites is essentially measured via in vitro cell culture or foliar spray, the large-scale production is significantly limited. Recent advancements in the plant cell culture technology lay the possibilities for the large-scale manufacturing of plant-derived secondary metabolites. With the insights about the genetic background of the metabolite biosynthetic pathway, synthetic biology also appears to be a potential avenue for accelerating their production. This review, therefore, also discussed the potential manoeuvres that can be deployed to synthesis plant secondary metabolites at the large-scale using plant cell, tissue, and organ cultures.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview

et al. 2022

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“…With the rapid development of the next generation sequencing, RNA sequencing (RNA-seq) is widely used in the analysis of transcriptomes of various organisms. The key genes of stilbene, flavonoid and anthraquinone biosynthesis are identified by tissue-specific transcriptome in the medicinal plant Polygonum cuspidatum ( Wang et al, 2021 ). The mechanism of anthocyanin biosynthesis is analyzed using metabolomics and transcriptomics in S. miltiorrhiza ( Liang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional regulation mechanism of flavonoids biosynthesis gene during fruit development in astragalus membranaceus

Suriguga

Zhao

et al. 2022

Front. Genet.

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Astragalus membranaceus, as an important medicinal plant, are an excellent source of flavonoids. Flavonoid compounds in A. membranaceus have been widely used in medicine and supplement, but known of the molecular mechanism of flavonoid biosynthesis is still very few. Here, we analyzed the association between flavonoid content and gene expression pattern during six different fruit developmental stages. Sixteen gene expression trends were significantly identified, involving 8,218 genes. The gene expression trend in profile 0 was positively correlated with flavonoid content, while the gene expression trend in profile 79 was negatively correlated with flavonoid content at six developmental stages. The expression level of genes involved in the general phenylpropane pathway was higher than that of genes involved in the flavonoid biosynthesis pathway. A total of 37 genes involved in flavonoid synthesis were identified in A. membranaceus. The expression pattern of flavonoid-related genes was highly correlated with flavonoid content. Our study deepened the understanding of the flavonoid synthesis mechanism and provided useful resources for future studies on the high flavonoid molecular breeding of A. membranaceus.

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“…With the development and maturity of next-generation and third-generation sequencing technologies, RNA-seq has been used to study the expression levels of key enzyme genes of a large number of medicinal plants in the biosynthesis of bioactive components, revealing the biosynthetic pathway and its regulatory mechanism of secondary metabolites ( Wang et al, 2021 ; Sun et al, 2021 ). There are 14 projects in the NCBI SRA relating to P. cablin , containing more than 100 runs in total.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of two Pogostemon cablin chemotypes reveals genes related to patchouli alcohol biosynthesis

Yan¹,

Ye²,

Cao³

et al. 2021

PeerJ

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Pogostemon cablin, a medicinally and economically important perennial herb, is cultivated around the world due to its medicinal and aromatic properties. Different P. cablin cultivars exhibit different morphological traits and patchouli oil components and contents (especially patchouli alcohol (PA) and pogostone (PO)). According to the signature constituent of the leaf, P. cablin was classified into two different chemotypes, including PA-type and PO-type. To better understand the molecular mechanisms of PA biosynthesis, the transcriptomes of Chinese-cultivated P. cablin cv. PA-type “Nanxiang” (NX) and PO-type “Paixiang” (PX) were analyzed and compared with ribonucleic acid sequencing (RNA-Seq) technology. We obtained a total of 36.83 G clean bases from the two chemotypes, compared them with seven databases and revealed 45,394 annotated unigenes. Thirty-six candidate unigenes participating in the biosynthesis of PA were found in the P. cablin transcriptomes. Overall, 8,390 differentially expressed unigenes were identified between the chemotypes, including 2,467 upregulated and 5,923 downregulated unigenes. Furthermore, six and nine differentially expressed genes (DEGs) were mapped to the terpenoid backbone biosynthetic and sesquiterpenoid and triterpenoid biosynthetic pathways, respectively. One key sesquiterpene synthase gene involved in the sesquiterpenoid and triterpenoid biosynthetic pathways, encoding patchoulol synthase variant 1, was significantly upregulated in NX. Additionally, GC-MS analysis of the two chemotypes in this study showed that the content of PA in NX was significantly higher than that of PX, while the content of PO showed the opposite phenotype. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that the DEG expression tendency was consistent with the transcriptome sequencing results. Overall, 23 AP2/ERF, 13 bHLH, 11 MYB, 11 NAC, three Trihelix, 10 WRKY and three bZIP genes that were differentially expressed may act as regulators of terpenoid biosynthesis. Altogether, 8,314 SSRs were recognized within 6,825 unigenes, with a distribution frequency of 18.32%, among which 1,202 unigenes contained more than one SSR. The transcriptomic characteristics of the two P. cablin chemotypes are comprehensively reported in this study, and these results will contribute to a better understanding of the molecular mechanism of PA biosynthesis. Our transcriptome data also provide a valuable genetic resource for further studies on P. cablin.

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Tissue-specific transcriptome analyses reveal candidate genes for stilbene, flavonoid and anthraquinone biosynthesis in the medicinal plant Polygonum cuspidatum

Cited by 24 publications

References 48 publications

Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview

Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview

Transcriptional regulation mechanism of flavonoids biosynthesis gene during fruit development in astragalus membranaceus

Transcriptome analysis of two Pogostemon cablin chemotypes reveals genes related to patchouli alcohol biosynthesis

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