Titania-coated fibrous silica (TiO2/KCC-1) core-shell microspheres based solid-phase extraction in clam (Corbicula fluminea) using hydrophilic interaction liquid chromatography and mass spectrometry

Zhao

Dai

et al. 2021

J. Agric. Food Chem.

Self Cite

Sea cucumber (Stichopus japonicus) is one of the most luxurious and nutritious seafoods in Asia. It is always processed into dried products to prevent autolysis, but its quality is easily destructed during storage. Herein, an extremely simplified workflow was established for real-time and in situ quality assessment of dried sea cucumbers (DSCs) during storage based on the lipid oxidation characteristics using an intelligent surgical knife (iKnife) coupled with rapid evaporative ionization mass spectrometry (REIMS). The lipidomic phenotypes of DSCs at different storage times were acquired successfully, which were then processed by multivariate statistical analysis. The results showed that the discrepancy in the characteristic ions in different DSCs was significant (p < 0.05) with high R 2(Y) and Q 2 values (0.975 and 0.986, respectively). The receiver operating characteristic curve revealed that the ions of m/z 739.5, m/z 831.5, m/z 847.6, and m/z 859.6 were the most specific and characteristic candidate biomarkers for quality assessment of DSCs during accelerated storage. Finally, this method was validated to be qualified in precision (RSDintraday ≤ 9.65% and RSDinterday ≤ 9.36%). In conclusion, the results showed that the well-established iKnife–REIMS method was high-throughput, rapid, and reliable in the real-time quality assessment of DSCs.

Section: Results and Discussionmentioning

confidence: 99%

In Situ Quality Assessment of Dried Sea Cucumber (Stichopus japonicus) Oxidation Characteristics during Storage by iKnife Rapid Evaporative Ionization Mass Spectrometry

Zhao

Dai

et al. 2021

J. Agric. Food Chem.

Self Cite

“…Circos map as a novel visual tool can identify and analyze the similarities and differences of samples, which can reveal the relationships between pairs of positions using the circular ideogram layout. Moreover, using histogram plots, connectors and lines display the correlation of the data by an aesthetic and intuitive way (Song, Wang, et al., 2020 ; Song, Zhu, et al., 2021 ). Figure 6d reveals that the strong correlation between the dominant phenols and flavonoids and the samples corresponded to the thickness of lines, indicating that 60‐mesh samples had a significant correlation with the detected phenols and flavonoids.…”

Section: Resultsmentioning

confidence: 99%

Effect of steam explosion pretreatment on the composition and bioactive characteristic of phenolic compounds in Chrysanthemum morifolium Ramat cv. Hangbaiju powder with various sieve fractions

Food Science & Nutrition

Liu

Shui

et al. 2022

Self Cite

“…Phospholipids were isolated from crude lipid extract using liquid–liquid extraction (LLE), and the procedure was described in detail in the Supporting Information . The fatty acyl profile of phospholipids was determined by transmethylation using methanol/sulfuric acid and gas chromatography (GC) analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The collected organic phases were combined and evaporated to dryness under nitrogen flow. Finally, the phospholipids were purified by titania-coated fibrous silica column chromatography …”

Section: Methodsmentioning

confidence: 99%

“…Marine phospholipids are important bioactive substances in multiple aspects in life science. , Recently, marine phospholipids, especially the unsaturated-fatty-acid-structured ones, have drawn widespread attention by researchers as a result of their significant nutritional characteristics . Omega-7 monoenoic fatty acids (n-7 FAs) widely exist in the brain, hepatic, white, and brown tissues, which are increasingly exploited as a result of the positive effects on human health and the great industrial potential .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Real-Time In Situ Screening of Omega-7 Phospholipids in Marine Biological Resources Using an iKnife-Rapid-Evaporative-Ionization-Mass-Spectrometry-Based Lipidomics Phenotype

Wang

Zhang

et al. 2021

J. Agric. Food Chem.

Self Cite

Omega-7 (n-7) phospholipids were bioactive substances in marine animals. In this study, a fast lipidomics phenotyping approach for real-time in situ screening of n-7 phospholipids in five kinds of economic seafood, salmon, prawn, bluefin tuna, hairtail, and butterfish, was established using iKnife rapid evaporative ionization mass spectrometry (REIMS). The n-7 phospholipids were structurally characterized and quantitatively analyzed, and the profiles were statistically analyzed by multivariate recognition analysis. It indicated that the difference of n-7 phospholipids in seafood samples was significant (p < 0.05), with R 2(cum) and Q 2(cum) values of >0.9. The proportion of n-7 phospholipids in salmon was the highest (20.43%), followed by bluefin tuna, prawn, hairtail, and butterfish. The ions of m/z 742.54 (PC 16:1–18:1), 768.55 (PC 16:1–20:2), 697.48 (PE 16:1–18:1), and 699.48 (PE 16:1–18:0) were the main n-7 phospholipids. The effectiveness of iKnife REIMS was further verified by hydrophilic interaction chromatography mass spectrometry and gas chromatography. The results demonstrated that proposed iKnife REIMS was an excellent technique for front-line screening of n-7 phospholipids in a large variety of marine biological resources.