TLC-densitometric method development and validation for the quantification of nicotine in tobacco smoked, sniffing, dipping, and chewing products

Abstract

A sensitive, selective, precise, and robust densitometric method for the analysis of nicotine (drug component of Nicotiana tabacum) in various tobacco products (TPS), including smoked (cigarette and beedi), sniffing (sniffing niswar), dipping (niswar), and chewing (gutka) tobacco brands, was developed and validated as per the ICH guideline. TLC glass plates, precoated with silica gel 60F-254, were used for the analysis. Densitometric analysis of nicotine was carried out in the absorbance mode at 262 nm. The mobile phase consisted of petroleum ether-acetone-diethylamine (7.6:2:0.4) v/v, and gave a sharp and symmetrical nicotine peak (R F = 0.57 ± 0.005) under saturated environment. Linear regression data (LRD) for the calibration curve showed a good linearity with r = 0.997 ± 0.0013. The method was validated for precision, recovery, and robustness. The limits of detection (LOD) and quantitation (LOQ) were found to be 3.15 and 9.54 ng per spot, respectively. Statistical analysis indicated that the method is reproducible and selective for the estimation of nicotine in various TPS.

HPTLC assay of nicotine and cotinine in biological samples

“…Changing the composition of the solvent system by replacing acetone with methanol, more polar solvent and reducing the amount of alkalizing agent provided the best separation of nicotine from cotinine (Rf values of 0.45 and 0.34, respectively). The Rf values fall within the acceptability condition on the deviation of ± 5% (16,17,18).…”

What Does TLC Mean?

“…TLC is also connected with many IT companies operating in industry, servicing constructions and giving engineering solution 2 , 3 , 4 , moreover comprehensive customer service connected with bearings in Poland (TLC Łożyska Sp.J.) 5 . Of all the meanings of TLC can be distinguished, those related to education goal like language school-Trinity Lutheran College, located in Everett, WA 6 .…”

“…In our research, planar chromatography was applied for assessment of tobacco smoke exposure to different group of people from Silesia Region in Poland by the use of earlier described chromatographic conditions [2,3]. Nicotine and its metabolites are rather really determined by planar chromatography [4][5][6], most often immunoassay has been used for screening and HPTLC for detail analysis [7].…”