2017
DOI: 10.1038/srep43455
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Tobacco vein banding mosaic virus 6K2 Protein Hijacks NbPsbO1 for Virus Replication

Abstract: Chloroplast-bound vesicles are key components in viral replication complexes (VRCs) of potyviruses. The potyviral VRCs are induced by the second 6 kDa protein (6K2) and contain at least viral RNA and nuclear inclusion protein b. To date, no chloroplast protein has been identified to interact with 6K2 and involve in potyvirus replication. In this paper, we showed that the Photosystem II oxygen evolution complex protein of Nicotiana benthamiana (NbPsbO1) was a chloroplast protein interacting with 6K2 of Tobacco … Show more

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Cited by 31 publications
(36 citation statements)
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“…In addition to viral RNA and ancillary replication factors, viruses recruit multiple host RNA-modifying enzymes, translation factors, RNA helicases, including DEAD-Box RNA helicases (in plants and yeast), and methyltransferase. The VRCs of different plant virus families reportedly might contain the following components: chaperones (facilitating proper folding of RNA), viral RNA, and proteins, host endosomal sorting complex required for transport (ESCRT) that participates also in membrane deformation ( Nagy and Pogany, 2012 ; Hyodo and Okuno, 2014 ; Geng et al, 2017 ), as well as other cellular proteins involved in lipid metabolism and transport ( Barajas et al, 2014 ).…”
Section: Requirement Of the Chloroplast Membrane For Plant Ss(+)rna Vmentioning
confidence: 99%
See 1 more Smart Citation
“…In addition to viral RNA and ancillary replication factors, viruses recruit multiple host RNA-modifying enzymes, translation factors, RNA helicases, including DEAD-Box RNA helicases (in plants and yeast), and methyltransferase. The VRCs of different plant virus families reportedly might contain the following components: chaperones (facilitating proper folding of RNA), viral RNA, and proteins, host endosomal sorting complex required for transport (ESCRT) that participates also in membrane deformation ( Nagy and Pogany, 2012 ; Hyodo and Okuno, 2014 ; Geng et al, 2017 ), as well as other cellular proteins involved in lipid metabolism and transport ( Barajas et al, 2014 ).…”
Section: Requirement Of the Chloroplast Membrane For Plant Ss(+)rna Vmentioning
confidence: 99%
“…Involvement of the oxygen-evolving complex in the regulation of virus replication has also been indicated. Geng et al (2017) experimentally proved that down-regulation of NbPsbO1 expression suppressed the replication of tobacco vein banding mosaic virus (TVBMV) as well as PVY. Additionally, the 6K2 protein was recently reported to recruit 6K1 and hijack the chloroplast protein NbPsbO1, a component of the photosystem II oxygen-evolving complex that regulates potyvirus replication; consistently virus accumulation is significantly decreased in NbPsbO1-silenced plants.…”
Section: The Role Of Host Proteins In Regulating the Viral Replicatiomentioning
confidence: 99%
“…In potyviruses, a small viral membrane protein 6K2 is involved in the local spatial re-arrangement of the ER membrane and the formation of vesicular VRC at the ER exit sites [59] , [65] , [73] [76] . Several other viral proteins including CI (replicative SF2 RNA helicase), 6K1, 6K2, NIa, HC-Pro, P3, and P3N-PIPO have been shown to be functional protein elements of VRC in addition to NIb [65] , [72] , [77] , [78] . The VRC with incorporated viral CP may work as the motile vesicles moving along actin filaments and involved in virus replication, the genome intercellular movement and localization on chloroplasts [42] , [65] , [76] , [79] , [80] .…”
Section: Potyvirusesmentioning
confidence: 99%
“…6K2 is able to create vesicles by itself even though it needs to interact with other viral and cellular proteins to create functional VRC (Löhmus et al. 2016; Geng et al. 2017).…”
Section: Introductionmentioning
confidence: 99%
“…2016). Using direct protein–protein interaction techniques (e.g., yeast two-hybrid, coimmunoprecipitation or BiFC), several other host proteins have been identified as 6K2 interactors, including the photosystem II oxygen evolution complex protein NtPsbO1 (Geng et al. 2017), the COPII coatomer component Sec24a protein (Jiang et al.…”
Section: Introductionmentioning
confidence: 99%