Tolerance to white spot syndrome virus (WSSV) in the freshwater prawn Macrobrachium rosenbergii is associated with low VP28 envelope protein expression

Yoganandhan, K.; Hameed, A.S. Sahul

doi:10.3354/dao073193

Cited by 15 publications

(14 citation statements)

References 37 publications

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“…A previous study comparing YHV challenge results in the black tiger shrimp (P. monodon) with those for various palaemonid shrimp indicated that tolerant palaemonid species carried YHV as persistent infections characterized by what appeared to be specific suppression of one of the YHV envelope proteins gp116 when compared with the capsid protein p20 [27]. A similar phenomenon has been reported for a plaemonid shrimp species that is tolerant to white spot syndrome virus (WSSV) infections [53]. In that case, production of the envelope protein vp28 appeared to be suppressed with time after challenge while the level of viral production remained unchanged.…”

Section: Article In Presssupporting

confidence: 54%

Low viral loads and lymphoid organ spheroids are associated with yellow head virus (YHV) tolerance in whiteleg shrimp Penaeus vannamei

Anantasomboon

Poonkhum

Sittidilokratna

et al. 2008

Developmental & Comparative Immunology

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Yellow head virus (YHV) is an invertebrate nidovirus that has caused mass mortality in penaeid shrimp since 1990. Several YHV types are known, but only the original type (YHV-type 1 or YHV-1) is highly virulent. Most studies have focused on acute YHV-1 infections and there is limited work on YHV-1 survivors. We compared moribund and surviving (14%) whiteleg shrimp Penaeus (Litopenaeus) vannamei from an experimental challenge with YHV-1. Although grossly normal, all survivors were positive for YHV-1 by specific, reverse transcriptase polymerase chain reaction (RT-PCR) assays, histological analysis or transmission electron microscopy (TEM), indicating that they were not resistant but tolerant to YHV-1. On the other hand, real-time PCR analysis revealed that mean YHV-1 copies/ng total RNA for survivors (2.8x10(4) +/- 6.9x10(4)) were approximately 40 times lower (P<0.05) than those in moribund shrimp (1.2x10(6) +/- 6.7x10(5)copies/ng total RNA). This was confirmed by strong positive immunohistochemical and in situ hybridization (ISH) reactions for YHV-1 in lymphoid organ tubules (LOT) of moribund shrimp and weak positive reaction only in lymphoid organ spheroids (LOS) of survivors. TEM revealed morphologically complete YHV virions in both groups. Furthermore, immuno-TEM and Western blot analysis revealed that YHV-1 structural proteins gp116 and p20 were present at comparable reactive levels in each group. Thus, YHV-1 tolerance was not associated with absence of gp116 as previously reported for palaemonid shrimp. Instead, it was associated with the presence of YHV-positive LOS and a relatively low viral load.

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Section: Article In Presssupporting

confidence: 54%

Low viral loads and lymphoid organ spheroids are associated with yellow head virus (YHV) tolerance in whiteleg shrimp Penaeus vannamei

Anantasomboon

Poonkhum

Sittidilokratna

et al. 2008

Developmental & Comparative Immunology

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“…The species affected can be Penaeus monodon, Marsupenaeus japonicus, Fenneropenaeus chinensis, F. indicus, F. merguiensis, Penaeus setiferus, P. stylirostris and P. vannamei (Cuéllar-Anjel, 2012). However, appa-rently Macrobrachium species such as M. rosenbergii are more tolerant to infection (Yoganandhan & Hameed, 2007). Estimated world economic losses associated to WSSV approach one billion US$ per year since its first report in 1992 (Flegel et al, 2008), so that probably an alternative was the Macrobrachium species culture, which brought the takeoff in research on this genus, making it a line of research in continuous growth.…”

Section: Resultsmentioning

confidence: 99%

Description of the scientific domain Macrobrachium through the analysis of the authors with higher productivity and higher H index according to Scopus

Chong-Carrillo

Moraes‐Valenti

Vega-Villasante

et al. 2018

LAJAR

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ABSTRACT. One of the most important domains in aquaculture is Macrobrachium, since it represents one of the most cultured crustacean genus worldwide. Description of the authors that study and publish on the subject constitutes a solid contribution to the knowledge of this genus. Indicators of productivity, visibility, relevance, sex and co-authorship of the authors were collected from the Scopus database for the period 1980-2016 to describe the Macrobrachium domain. Conceptual mapping techniques were used to visualize co-author relationships. The greatest amount of contributions to knowledge of the genus Macrobrachium was shown to come from researchers in China, Brazil, USA and India. Only seven women are among the most productive and relevant authors. Multiple co-author networks exist in which the most relevant researchers are the center. Many networks appear to be closed groups with little interaction between external groups or researchers.

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“…For LFIA, 40μL of each concentration was applied to the assay strip. For one-step PCR, DNA was isolated from the above protein lysates (1 mL) and amplified using commercial PCR kit for WSSV supplied by Poseidon Biotech, Chennai, India [27]. …”

Section: Methodsmentioning

confidence: 99%

Field-Usable Lateral Flow Immunoassay for the Rapid Detection of White Spot Syndrome Virus (WSSV)

Kulabhusan

Rajwade

Vimal³

et al. 2017

PLoS ONE

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BackgroundWhite spot disease (WSD), a major threat to sustainable aquaculture worldwide, is caused by White spot syndrome virus (WSSV). The diagnosis of WSD relies heavily on molecular detection of the virus by one-step PCR. These procedures are neither field-usable nor rapid enough considering the speed at which the virus spreads. Thus, development of a rapid, reliable and field-usable diagnostic method for the detection of WSSV infection is imperative to prevent huge economic losses.Methods/Principal FindingsHere, we report on the development of a lateral flow immunoassay (LFIA) employing gold nanoparticles conjugated to a polyclonal antibody against VP28 (envelope protein of WSSV). The LFIA detected WSSV in ~20 min and showed no cross-reactivity with other shrimp viruses, viz. Monodon Baculovirus (MBV), Hepatopancreatic parvovirus (HPV) and Infectious Hypodermal and Hematopoietic Necrosis virus (IHHNV). The limit of detection (LOD) of the assay, as determined by real-time PCR, was 103 copies of WSSV. In a time course infectivity experiment, ~104 WSSV particles were injected in Litopenaeus vannamei. The LFIA could rapidly (~ 20 min) detect the virus in different tissues after 3 h (hemolymph), 6 h (gill tissue) and 12 h (head soft tissue, eye stalk, and pleopod) of infection. Based on these findings, a validation study was performed using 75 field samples collected from different geographical locations in India. The LFIA results obtained were compared with the conventional “gold standard test”, viz. one-step PCR. The analysis of results in 2x2 matrix indicated very high sensitivity (100%) and specificity (96.77%) of LFIA. Similarly, Cohen’s kappa coefficient of 0.983 suggested "very good agreement” between the developed LFIA and the conventional one-step PCR.ConclusionThe LFIA developed for the rapid detection of WSSV has an excellent potential for use in the field and could prove to be a boon to the aquaculture industry.

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Tolerance to white spot syndrome virus (WSSV) in the freshwater prawn Macrobrachium rosenbergii is associated with low VP28 envelope protein expression

Cited by 15 publications

References 37 publications

Low viral loads and lymphoid organ spheroids are associated with yellow head virus (YHV) tolerance in whiteleg shrimp Penaeus vannamei

Low viral loads and lymphoid organ spheroids are associated with yellow head virus (YHV) tolerance in whiteleg shrimp Penaeus vannamei

Description of the scientific domain Macrobrachium through the analysis of the authors with higher productivity and higher H index according to Scopus

Field-Usable Lateral Flow Immunoassay for the Rapid Detection of White Spot Syndrome Virus (WSSV)

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