Tolerancia oral en artritis experimental inducida por antígeno en conejos por administración de hidrolizado de cartílago articular

Abramson, David B.; Cabello, Julieta; Bumaguin, G.E.; Jamin, A.; Vitelli, Ezequiel J.; Zingoni, N.; Sarrió, Leandro; Feldman, Sara; Cointry, Gustavo Roberto

doi:10.1016/j.inmuno.2014.06.003

Cited by 3 publications

(4 citation statements)

References 29 publications

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“…Clinical observation showed significant differences between rabbits with induced arthritis (A) versus controls, as reported previously (Abramson et al, ; Mortarino et al, ; Toledo et al, ). The simultaneous treatment of collagen hydrolysates cholecalciferol significantly reduced clinical scores of rabbits, not only in comparison with group A, but also with those obtained with those animals of the groups that received a single treatment (Fig.…”

Section: Resultssupporting

confidence: 80%

“…Twenty days after the second induction, we verified whether there was inflammatory state in rabbits subjected to arthritis induction, by clinical studies and magnetic resonance (MR) studies. This procedure allowed us to avoid unnecessary slaughter of animals and to analyze the synovial tissue anatomopathologically at this stage, as previously performed (Abramson et al, ). The MR studies were performed to confirm that all knees of the animals presented hydroarthrosis in the articular space and alterations in the subchondral bone, detected as hyperintensity.…”

Section: Methodsmentioning

confidence: 99%

“…Three independent observers studied 50 fields per sample. Criteria for characterization of the synovial membrane fields used in previous work were applied, in high power field; (Abramson et al, ): normal synovial tissues (without inflammatory cells), low inflammation tissue (<5 inflammatory cells/field), moderate inflammation tissue (5–10 inflammatory cells/field, and edema), severely inflamed tissue (more than 15 inflammatory cells/field, and angiogenesis and edema).…”

Section: Methodsmentioning

confidence: 99%

“…We have presented preliminary evidence in our model of antigen‐induced arthritis, in which oral tolerance treatment with hydrolyzed collagen would decrease the progression of the inflammatory pathology (Abramson et al, ; Mortarino et al, ; Toledo et al, ). On the other hand, vitamin D, as a pleiotropic hormone, may synergize the immunotolerance process (because of the evidence that low levels of vitamin D are related to a decrease in the proportion of TCD4+ cells that would differentiate towards the formation of regulatory or suppressor T cells, directly related to tolerance phenomena) (Christakos et al, ).…”

Section: Introductionmentioning

confidence: 97%

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Emerging therapy in arthritis: Modulation of markers of the inflammatory process

Mortarino

Goy

Abramson

et al. 2016

Microscopy Res & Technique

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The induction of tolerance has been proposed as a therapeutic strategy for arthritis aiming to decrease progression of the pathology, probably by promoting suppressor mechanisms of the autoimmune response. This work aimed to confirm whether the treatment with vitamin D3 could synergize oral tolerance induced by hydrolyzed collagen peptides, in our experimental model of antigen induced arthritis in New Zealand rabbits. Clinical observation of the phenomenon indicates that simultaneous treatment with hydrolyzed collagen peptides and vitamin D3 was beneficial when compared with no treatment, for arthritic animals, and for arthritic animals that received treatment with only hydrolyzed collagen peptides or vitamin D3. Treatment with hydrolyzed collagen peptides caused diminished proinflammatory cytokine levels, an effect synergized significantly by the simultaneous treatment with vitamin D3. The anatomical-pathological studies of the animals that received both treatments simultaneously showed synovial tissues without lymphocytic and plasma cell infiltrates, and without vascular proliferation. Some of the synovial tissue of the animals of these groups showed a slight decrease in Galectin-3 expression. We propose that simultaneous oral treatment with vitamin D3 and hydrolyzed collagen peptides could increase the immunoregulatory effect on the process of previously triggered arthritis. We used articular cartilage hydrolysate and not collagen II because peptides best expose antigenic determinants that could induce oral tolerance. Oral tolerance may be considered in the design of novel alternative therapies for autoimmune disease and we have herein presented novel evidence that the simultaneous treatment with vitamin D3 may synergize this beneficial effect.

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Section: Resultssupporting

confidence: 80%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

Emerging therapy in arthritis: Modulation of markers of the inflammatory process

Mortarino

Goy

Abramson

et al. 2016

Microscopy Res & Technique

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Boron neutron capture synovectomy (BNCS) as a potential therapy for rheumatoid arthritis: radiobiological studies at RA-1 Nuclear Reactor in a model of antigen-induced arthritis in rabbits

Trivillin

Bruno

Gatti

et al. 2016

Radiat Environ Biophys

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Rheumatoid arthritis is a chronic autoimmune pathology characterized by the proliferation and inflammation of the synovium. Boron neutron capture synovectomy (BNCS), a binary treatment modality that combines the preferential incorporation of boron carriers to target tissue and neutron irradiation, was proposed to treat the pathological synovium in arthritis. In a previous biodistribution study, we showed the incorporation of therapeutically useful boron concentrations to the pathological synovium in a model of antigen-induced arthritis (AIA) in rabbits, employing two boron compounds approved for their use in humans, i.e., decahydrodecaborate (GB-10) and boronophenylalanine (BPA). The aim of the present study was to perform low-dose BNCS studies at the RA-1 Nuclear Reactor in the same model. Neutron irradiation was performed post intra-articular administration of BPA or GB-10 to deliver 2.4 or 3.9 Gy, respectively, to synovium (BNCS-AIA). AIA and healthy animals (no AIA) were used as controls. The animals were followed clinically for 2 months. At that time, biochemical, magnetic resonance imaging (MRI) and histological studies were performed. BNCS-AIA animals did not show any toxic effects, swelling or pain on palpation. In BNCS-AIA, the post-treatment levels of TNF-α decreased in four of six rabbits and IFN-γ levels decreased in five of six rabbits. In all cases, MRI images of the knee joint in BNCS-AIA resembled those of no AIA, with no necrosis or periarticular effusion. Synovial membranes of BNCS-AIA were histologically similar to no AIA. BPA-BNCS and GB-10-BNCS, even at low doses, would be therapeutically useful for the local treatment of rheumatoid arthritis.

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Immunomodulatory role of edible bone collagen peptides on macrophage and lymphocyte cell cultures

Soriano-Romaní

Nieto

García-Benlloch

2022

Food and Agricultural Immunology

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Tolerancia oral en artritis experimental inducida por antígeno en conejos por administración de hidrolizado de cartílago articular

Cited by 3 publications

References 29 publications

Emerging therapy in arthritis: Modulation of markers of the inflammatory process

Emerging therapy in arthritis: Modulation of markers of the inflammatory process

Boron neutron capture synovectomy (BNCS) as a potential therapy for rheumatoid arthritis: radiobiological studies at RA-1 Nuclear Reactor in a model of antigen-induced arthritis in rabbits

Immunomodulatory role of edible bone collagen peptides on macrophage and lymphocyte cell cultures

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