Tomography System to Acquire 3D Images of Cells in Laminar Flow: Hardware Architecture

Abate,; Bavaro,; Castello,; Daponte, P.; Grimaldi, D.; Guglielmelli, Eugenio; Martinelli,; Mauro,; Moisa,; Napolitano,; Rapuano,; Scerbo,

doi:10.1109/memea.2006.1644463

Cited by 10 publications

(7 citation statements)

References 13 publications

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“…In the paper the proposal to improve the effectiveness and the reliability of the Image Flow Cytometer (IFC) pointed out in [1]- [2] is presented. The hardware architecture of the actual realization of the IFC is discussed to highlight the causes of the alterations affecting the acquired images.…”

Section: Discussionmentioning

confidence: 99%

“…The core of the entire device is the flow chamber (Fig.3), in which lymphocyte cells are injected in a serial flow stream, by means of high precision electronic pump and proportional "needle" valves [1]. The image acquisition system is constituted by: (i) control unit, (ii) image capturing device, and (iii) secondary excitation light source.…”

Section: Ifc Hardware Architecturementioning

confidence: 99%

“…In particular, the synchronization among the flow of the lymphocyte, the light, and the image acquisition is performed under the hypothesis of uniform laminar flow [1]. Spike in the power of the pump, density variation of the fluid, vibrations misstate this hypothesis introducing random delay and, consequently, image alterations.…”

Section: Ifc Hardware Architecturementioning

confidence: 99%

“…The Image Flow Cytometer (IFC) was proposed to acquire the cell images of the lymphocyte, and to detect and count the Micro Nucleuses (MNs) [1], [2]. The MN frequency induced in the cells is widely used for analysis of cytogenetic damage.…”

Section: Introductionmentioning

confidence: 99%

“…This hypothesis is misstated by spikes in the flow speed introduced by the hydraulic pump system, and opening and closing of valve used to control the lymphocyte flow [1].…”

Section: Introductionmentioning

confidence: 99%

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Synchronization in the image Flow Cytometer

Lamonaca

Grimaldi

Nastro

2011

Proceedings of the 6th IEEE International Conference on Intelligent Data Acquisition and Advanced Computing Systems

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The Image Flow-Cytometer permits to acquire the cell images of the lymphocytes. These images are used to count the Micro Nucleuses (MNs) induced in the cells by carcinogenic agents. The result of the count is widely used for analysis of cytogenetic damage. The acquisition is performed under the hypothesis of laminar flow of the lymphocyte. Nevertheless, many are the causes that make invalid this hypothesis and cause alterations during the image acquisition. The final effect is the presence of different alterations superimposed to the images, that misstate the algorithms used to detect the MNs. In order to reduce the number of images affected by not admissible alteration range, and to increase the reliability of the medical response, a new hardware system is pointed out to synchronize with high accuracy the image acquisition to the lymphocyte flow.

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Section: Discussionmentioning

confidence: 99%

Section: Ifc Hardware Architecturementioning

confidence: 99%

Section: Ifc Hardware Architecturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…This hypothesis is misstated by spikes in the flow speed introduced by the hydraulic pump system, and opening and closing of valve used to control the lymphocyte flow [1].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations