Topical application of HIV DNA vaccine with cytokine-expression plasmids induces strong antigen-specific immune responses

Liu, Lijuan; Watabe, Setsuko; Yang, Jun; Hamajima, Kenji; Ishii, Norihisa; Hagiwara, Eri; Onari, Katsuhiro; Xin, Ke-Qin; Okuda, Kenji

doi:10.1016/s0264-410x(01)00324-3

Cited by 39 publications

(33 citation statements)

References 27 publications

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“…In addition to serum IgG, fecal IgA (mucosal immunity) was also observed on topical application of DNA. This result was consistent with the findings of Glenn et al [28,29], but in contrast to those of Liu et al [22]. Topical DNA vaccine also induced a substabtial level of antigen-specific cytotoxic T lymphocyte (CTL) responses in stripped skin: 37% at an effector:target (E:T) ratio of 80:1.…”

Section: Stripping Versus Immune Responsesupporting

confidence: 92%

“…Nishijima et al [52] reported that disruption of the skin barrier results in epidermal Langerhans cell activation as vigorous antigen presenters for T helper cells. Furthermore, Liu et al [22] reported that the stripped skin sections showed more S-100 protein staining and that neutrophil-like cell infiltration was greater and more frequently observed. S-100 protein is characteristic of mature dendritic cells (Langerhans cells) that are effective in antigen presentation.…”

Section: Mechanism Of Topical Dna Vaccinesmentioning

confidence: 99%

“…immune response without stripping [15,22,23]. Hence, the mechanism of DNA delivery into skin requires elaboration.…”

Section: Skin Barrier and Pdna Penetrationmentioning

confidence: 99%

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Topical Vaccination of DNA Antigens: Topical Delivery of DNA Antigens

Choi

Maibach²

2003

Skin Pharmacol Physiol

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Topical DNA vaccines have been shown to elicit both broad humoral and cellular immune responses in vivo. The skin is an attractive site for the delivery of DNA antigens for DNA vaccination. However, due to skin’s barrier properties, the penetration of DNA and the applications of topical vaccination are limited. To improve permeability, chemical and physical approaches have been examined to decrease stratum corneum barrier properties. Topical vaccination has been achieved using topical application of naked DNA, DNA/liposomes or emulsion complex, liposomal cream, as well as physical methods such as stripping, electroporation, and micromechanical disruption methods. All methods resulted in a significant enhancement in humoral and cellular immune responses over naked DNA alone. To develop more cost-effective and needle-free vaccines, skin-targeted immunizations are required. This review focuses on the chemical and physical methods developed to enhance DNA delivery into skin.

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Section: Stripping Versus Immune Responsesupporting

confidence: 92%

Section: Mechanism Of Topical Dna Vaccinesmentioning

confidence: 99%

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Topical Vaccination of DNA Antigens: Topical Delivery of DNA Antigens

Choi

Maibach²

2003

Skin Pharmacol Physiol

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“…In addition to peptide and protein antigen, tape stripping increased the humoral and cellular immune responses of topical DNA antigens [27,28]. Comparing the immune response with and without stripping, topical application without stripping induced a weak antibody response and did not elicit a sufficient CTL response.…”

Section: Tape Stripping and Topical Vaccinationmentioning

confidence: 98%

Effect of Tape Stripping on Percutaneous Penetration and Topical Vaccination

et al. 2003

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The stratum corneum provides the first barrier to the percutaneous absorption of drugs as well as regulating water loss. This barrier limits the topical/transdermal delivery of drugs and biological macromolecules. Chemical and physical approaches have been examined to decrease these properties. Tape stripping is commonly used to disrupt the epidermal barrier, to enhance the delivery of drugs and to obtain information about stratum corneum function. Tape stripping results in the production and release of cytokines and co-stimulatory molecules and increases the humoral and cellular immune responses against peptide, protein and DNA antigens by a topical vaccination in vivo. This paper reviews the stripping method, experimental factors and its applications for penetration and topical vaccination.

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“…In another study, co-administration of the HIV-1 DNA vaccine with pIL-12 and pGM-CSF by topical application to the skin enhanced the levels of both the HIV-specific cytotoxic T lymphocyte response and delayed-type hypersensitivity (DTH). Indeed, the skin is accessible for generating immune responses by both intradermal injection and topical use of gene delivery vectors [30]. In addition, co-administration of plasmids encoding the codon-optimized GM-CSF sequence with the HIV-1 Gag DNA vaccine resulted in a strong antibody and CTL response and a protective immune response against infection with recombinant vaccinia virus expressing HIV-1 Gag [31].…”

Section: Adjuvantsmentioning

confidence: 99%

How can Improve DNA Vaccine Modalities as a Therapeutic Approach against HIV Infections?

Habibzadeh¹

2015

J AIDS Clin Res

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Topical application of HIV DNA vaccine with cytokine-expression plasmids induces strong antigen-specific immune responses

Cited by 39 publications

References 27 publications

Topical Vaccination of DNA Antigens: Topical Delivery of DNA Antigens

Topical Vaccination of DNA Antigens: Topical Delivery of DNA Antigens

Effect of Tape Stripping on Percutaneous Penetration and Topical Vaccination

How can Improve DNA Vaccine Modalities as a Therapeutic Approach against HIV Infections?

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