Topical Delivery of Lactobacillus Culture Supernatant Increases Survival and Wound Resolution in Traumatic Acinetobacter baumannii Infections

Stanbro, Josh; Park, Ju Me; Bond, Matthew C.; Stockelman, Michael; Simons, Mark P.; Watters, Chase

doi:10.1007/s12602-019-09603-z

Cited by 18 publications

(19 citation statements)

References 57 publications

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“…In a study conducted in the United States [ 44 ], topical treatment with the supernatants of Lactobacillus acidophilus , L. casei , and L. reuteri was used on mouse wounds infected with A. baumannii . The results showed that the antimicrobial and anti-inflammatory effects of probiotics with local treatment could enhance wound healing [ 44 ]. The study, in general, demonstrated that the topical application of some Lactobacillus species can be effective against the Gram-negative pathogen A. baumannii .…”

Section: Discussionmentioning

confidence: 99%

“…Acinetobacter baumannii is the most common and harmful nosocomial pathogen causing human infections globally, particularly in critical care units (ICUs) [ 7 ]. Infections induced by A. baumannii commonly lead to substantial rates of morbidity and mortality, reaching up to 60%, including ventilator-associated pneumonia (VAP), septicemia, meningitis, endocarditis, urinary tract infection (UTI), keratitis, and ophthalmitis [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Identification of Effective Probiotics on Drug-Resistant Acinetobacter baumannii Strains and Their Biofilms

Abbasi,

Ghasemi,

Ahmadi

et al. 2024

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Introduction. This study aimed to identify, assess, and isolate strong lactobacilli demonstrating broad antibacterial and anti-biofilm activity against drug-resistant strains of Acinetobacter baumannii. Additionally, the mechanism of inhibition of these organisms was to be determined. Methods. Over a 6-month period (from December 2021 to June 2022), 53 clinical A. baumannii strains were collected from clinical samples. Twenty probiotic strains were isolated from local dairy products. Antibacterial activity of Lactobacillus strains’ cell-free supernatant (CFS) was identified using the agar well diffusion method and the microbroth dilution test. Anti-biofilm effect was performed by the microtiter plate assay. The MTT assay was also used to look into the probiotics’ cytotoxic effects on the L929 fibroblast cell line. Results. During the 6-month period, 53 clinical A. baumannii strains were obtained and identified. Out of 20 lactobacillus strains, the CFS of a lactobacillus strain (named L9) showed an inhibitory effect against all A. baumannii strains. Using the broth microdilution method, it was shown that the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of CFS extracts of L9 strains against A. baumannii strains were both ¼ mg/mL. The result of the anti-biofilm showed that the selected probiotic could inhibit biofilm formation. The most common organic acid produced by all Lactobacillus strains, according to the HPLC method, was lactic acid, which was followed by acetic acid. The L929 fibroblast cell line was used in the cytotoxicity assay, which revealed that 100% of the cells in the L929 fibroblast cell line survived treatment with successive doses of CFSs for a full day. Conclusion. The probiotic strain isolated from local yogurt in this study showed potential anti-biofilm and antimicrobial properties against all drug-resistant Acinetobacter isolates. Given the increasing interest in probiotic microorganisms based on their high health benefits, further studies are recommended on the mechanisms of action between probiotics and A. baumannii strains to find new solutions for biological control and treatment of these infections without the use of antibiotics.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Effective Probiotics on Drug-Resistant Acinetobacter baumannii Strains and Their Biofilms

Abbasi,

Ghasemi,

Ahmadi

et al. 2024

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…It was also reported in several studies that long-term cultivation of LAB strains may have a strong inhibition effect on pathogenic bacteria due to produce carbon dioxide (CO 2 ) while growing. Some researchers indicated that supernatants extracted from ve-day [12] and 3-day cultures [40] were utilized to allow for the maximal accumulation of antimicrobial compounds. In this study, when the inhibitory activities of CFSs, which were obtained from 24, 48, and 72 hr incubation, were compared, the highest inhibitory activities were found CFS-72.…”

Section: Discussionmentioning

confidence: 99%

“…During the bacterial proliferation of LAB, inhibitory compounds which include organic acids, hydrogen peroxide, antimicrobial peptides, and bacteriocins may be secreted into Cell-free supernatant (CFS) and it is likely a source of antimicrobial activity against many pathogenic bacteria [10]. There are very few studies investigating the antibio lm properties of CFSs [11,12]. Therefore, the investigation of antibacterial and antibio lm properties of CFSs is thought to be useful in revealing the probiotic potential of LAB strains.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the potential probiotic effects of lactic acid bacteria and cell-free supernatants against important pathogens leading to wound infections

Yilmaz¹,

TURKYILMAZ²

2023

Minerva Biotechnol Biomol Res

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In recent years, several in vitro studies have revealed that some of the LAB strains and their Cell-free supernatants (CFSs) have shown antibacterial activities and promising tools to prevent or disperse the bio lm formation of dermatological pathogens. In this study, it was aimed to investigate the antibacterial effects of LAB active cultures and CFSs against P. aeruginosa and S. aureus which were related to wound infections, as well as the inhibitory effects of CFSs on the bio lm formation of pathogens and destroying bio lms. The current results indicated that some of the LAB active cultures and CFSs have shown antibacterial properties against pathogens; furthermore, it was determined that some of the CFSs have shown powerful inhibitory effects on bio lm formation and destroying bio lms. All data together signify a great ability of the applications of CFSs as antibacterial and bio lm removal compounds that could be very suitable to control wound infections caused by these pathogens.

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“…acidilactici BNS5B) and zingerone were determined against Ps. aeruginosa PAO1 by broth microdilution method following the criteria of Clinical and Laboratory Standards Institute with some modifications [33]. The tested concentration of CFS and biogenics ranged from 0.703 to 90%, whereas 2 to 256 mg/mL was the range for zingerone.…”

Section: Mic Determination and Sub-mic Selection Of Cfs Zingerone And Biogenicsmentioning

confidence: 99%

Synergistic Effect of Biogenics Derived from Potential Probiotics Together with Zingerone Against Biofilm Formation by Pseudomonas aeruginosa PAO1

Chandla

Harjai

Shukla

2021

Probiotics & Antimicro. Prot.

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Biogenics are compounds produced by living organisms such as animals, plants, bacteria, etc. Probiotics and their biogenics are known for their antimicrobial potential. Therefore, the present study was designed to evaluate the antibiofilm potential of probiotic-derived biogenics in conjunction with zingerone against the Pseudomonas aeruginosa PAO1 biofilm. Cell-free supernatant (CFS) of potential probiotics Pediococcus acidilactici BNS5B and Lactobacillus fermentum PUM was found to inhibit the growth of Ps. aeruginosa PAO1 maximally among the nineteen isolated lactic acid bacteria. L. fermentum PUM produced precipitated protein fraction (PP), organic acids (OAs), exopolysaccharides (EPSs), biosurfactants (BSs) and various volatile antimicrobial compounds, while Ped. acidilactici BNS5B was found to produce PP, OA, EPS, BS and fewer volatile antimicrobial compounds only. More specifically, CFS and selected biogenics (OA and PP from L. fermentum PUM; OA from Ped. acidilactici BNS5B) of both potential probiotics showed synergy with zingerone against Ps. aeruginosa growth as observed by FIC index (< 0.5). Interestingly, CFS of both potential probiotics in combination with zingerone led to the formation of a more distorted biofilm compared with OA of L. fermentum PUM and zingerone, OA of Ped. acidilactici BNS5B and zingerone, PP of L. fermentum PUM and zingerone as well as their individual counterparts. Similarly, both confocal laser scanning microscopy and XTT assay showed an increased number of dead and impaired cells along with the decreased viability of biofilm cells. Thus, it can be reckoned that a combination of probiotic-derived biogenics and zingerone can have therapeutic application against Ps. aeruginosa infections which needs to be validated clinically.

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Topical Delivery of Lactobacillus Culture Supernatant Increases Survival and Wound Resolution in Traumatic Acinetobacter baumannii Infections

Cited by 18 publications

References 57 publications

Isolation and Identification of Effective Probiotics on Drug-Resistant Acinetobacter baumannii Strains and Their Biofilms

Isolation and Identification of Effective Probiotics on Drug-Resistant Acinetobacter baumannii Strains and Their Biofilms

Investigation of the potential probiotic effects of lactic acid bacteria and cell-free supernatants against important pathogens leading to wound infections

Synergistic Effect of Biogenics Derived from Potential Probiotics Together with Zingerone Against Biofilm Formation by Pseudomonas aeruginosa PAO1

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