Topical lipophilic epigallocatechin-3-gallate on herpes labialis: a phase II clinical trial of AverTeaX formula

Zhao, Man; Zheng, Rong; Jiang, Jingjing; Dickinson, Douglas; Fu, Baiping; Chu, Tin-Chun; Lee, Lee Hwang; Pearl, Henna; Hsu, Stephen

doi:10.1016/j.oooo.2015.08.018

Cited by 15 publications

(15 citation statements)

References 29 publications

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“…We reported previously that 50 µM EGCG-p completely blocks the infectivity of herpes simplex-1 virus (HSV-1) in Vero cells; this compound showed significantly higher efficacy than watersoluble EGCG [19]. The antiviral effect of lipophilic EGCG was further validated in a clinical trial treating herpes labialis caused by herpes simplex virus; it demonstrated high efficacy, without reported side effect [11,12]. Newly published data from hand sanitizer formulation testing demonstrated that, using 50% Tissue Culture Infective Dose (TCID50) assay methods, a novel alcoholbased sanitizer formulation containing EGCG-p resulted in a mean 6-log 10 -reduction of human poliovirus 1 (PV-1) infectivity (i.e., one million-fold reduction), exceeding by 100-fold the mandatory in vitro >4 log 10 -reduction of PV-1 infectivity for virucidal agents set by the US (EPA), EU, Canada and China [22].…”

Section: Introductionmentioning

confidence: 98%

“…Previous studies have indicated that epigallocatechin-3-gallate (EGCG) and its lipid-soluble derivatives, especially EGCG-palmitate (EGCG-p), could be strong candidates for the next generation of antiviral and virucidal materials [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. EGCG is a major polyphenol component of extracts from dry leaves of the Camellia sinensis (tea) plant, and its antiviral activities have been studied widely.…”

Section: Introductionmentioning

confidence: 99%

“…Due to their stability, global antiviral properties, high efficacy, persistency, and absence of known toxicity under normal doses, lipophilic EGCG compounds become the most suitable derivatives of EGCG for topical applications [18,20]. A group of lipophilic EGCG compounds has been tested, with promising results, in multiple in vitro and in vivo models against influenza A virus, herpes simplex 1 virus, and polio virus 1 [3,11,12,19,22].…”

Section: Introductionmentioning

confidence: 99%

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Persistent Virucidal Activity in an Alcohol-Based Sanitizer Formulation (ProtecTeaV) for Potential Use against Norovirus

Widjaja¹,

Dickinson²,

Shao³

et al. 2018

Microbiol Infect Dis

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Background: Norovirus is a major cause of acute gastroenteritis. Alcohol sanitization is ineffective, and currently used alcohol-based hand sanitizers are not recommended by the CDC for norovirus in healthcare settings. This study evaluated virucidal activity and surface persistence of a novel alcohol-based hand sanitizer formulation, ProtecTeaV, containing lipophilic epigallocatechin-3-gallate (EGCG-p) against a human norovirus surrogate.Methods: Virucidal capacity against feline calicivirus (FCV) was tested using a standard 50% Tissue Culture Infective Dose (TCID50) suspension assay. Persistence of residual virucidal activity after application on a clean surface was determined through 12 hours. Controls included the formulation without EGCG-p, popular alcoholbased sanitizers, and antibacterial liquid hand soap (LHS). Statistical analysis employed one-way ANOVA (alpha=0.05).Results: Suspension assays demonstrated that the ProtecTeaV formulation effectively reduced FCV viral infectivity >log 10 4 (10,000 fold). Surface applied residue activity remained strong (reduction of infectivity by > log 10 3) through 12 hours. In comparison, LHS did not show virucidal activity without washing with water, and other controls failed to reduce infectivity by more than log 10 3 (1,000 fold). Conclusion:This non-toxic hand sanitizer/surface disinfectant demonstrated effective and prolonged virucidal activities against a norovirus surrogate. Therefore, the EGCG-p formulation is potentially a novel and effective approach to curtail norovirus outbreaks. Research ArticleCitation: Widjaja Nicole, Dickinson Douglas, Shao Xueling, et al. Persistent Virucidal Activity in an Alcohol-Based Sanitizer Formulation (ProtecTeaV) for Potential Use against Norovirus. Microbiol Infect Dis. 2018; 2(2): 1-8.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Persistent Virucidal Activity in an Alcohol-Based Sanitizer Formulation (ProtecTeaV) for Potential Use against Norovirus

Widjaja¹,

Dickinson²,

Shao³

et al. 2018

Microbiol Infect Dis

View full text Add to dashboard Cite

show abstract

“…From various derivatives of EGCG, Kaihatsu's group identified a lipid-soluble EGCG, EGCG-mono-palmitate, as the most effective form of EGCG to inactivate influenza virus, being 44-fold more effective than water-soluble EGCG [16]. Previous studies from our group demonstrated that EGCGpalmitate (EC16) is more potent than EGCG for herpes simplex virus 1 (HSV-1) inactivation, and the clinical outcome from topical application of the EC16-containing formulation AverTeaX for herpes labialis was superior to other topical medications against herpes labialis [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Effect of Novel Formulations using Lipophilic Epigallocatechin-3-Gallate against Influenza Virus Infection

Dickinson¹,

Xayaraj²,

Dickinson³

et al. 2018

Microbiol Infect Dis

Self Cite

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Influenza virus infection is associated with high morbidity and mortality, and effective prophylactic and therapeutic methods to reduce infection outbreaks are lacking. Vaccination and current prevention/treatment approaches are not associated with a decline in morbidity or mortality, either globally or in the United States. Neither vaccination nor current anti-viral drugs prevent viral entry into the body and host cells, but rather target post-infection events in individuals. Hand washing and sanitizing do not prevent aerosol viral transmission, which accounts for the majority of influenza virus infections. Therefore, protection of the epithelia of the respiratory tract from viral entry is a significant gap in preventive strategies that needs to be filled. We hypothesize that lipophilic epigallocatechin-3-gallate (L-EGCG), and EGCG-palmitate (EC16) in particular, is able to fill this gap and become a first-in-class prophylactic and therapeutic approach against influenza virus infection. The objective of the current study was to investigate a proof-of-concept for the use of EC16 to prevent and treat influenza virus infection. The experimental design included direct contact of formulations containing EC16 with H1N1 influenza virus prior to infection assay (TCID50) in MDCK cells, incubation of cells with EC16 formulations either before or after H1N1 viral infection (without direct formulation contact with the virus), and coating the cell surface with EC16 formulations prior to H1N1 viral infection, followed by TCID50 assays. The results demonstrated that at a 0.1% concentration, EC16 formulations were effective (>95%) in blocking H1N1 infection regardless of direct contact with the virus. In conclusion, formulations containing EC16 could be an effective prophylactic and therapeutic approach to combat influenza infection in the respiratory tract, pending further in vitro and in vivo studies.

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“…Preventive effects of green tea polyphenols against photoaging are mediated by antimelanogenic and antiwrinkle mechanisms and by the inhibition of UV-induced immunosuppression [23]. Furthermore, green tea polyphenols possess antiviral properties and have been found effective in the treatment of herpes simplex labialis infections [24] and condylomata accuminata [25]. These findings suggest that green tea may be suitable for the prevention and treatment of various skin diseases.…”

Section: Introductionmentioning

confidence: 99%

A Randomized Controlled Trial of Green Tea Beverages on the in vivo Radical Scavenging Activity in Human Skin

Megow¹,

Darvin²,

Meinke³

et al. 2017

Skin Pharmacol Physiol

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Background: Oxidative stress plays an important role in the pathogenesis of various skin diseases. Thus, the antioxidant network of the skin relies on the uptake of exogenous antioxidants to ensure cell protection against radical formation. Green tea is one of the main sources of polyphenolic antioxidant compounds, but only few data are available on its cutaneous antioxidant effects. Methods: The radical scavenging properties of Benifuuki and Yabukita green tea were investigated in the human skin. Thirty-two participants who met the inclusion criteria were randomized to consume 3 cups per day of either Benifuuki tea, Yabukita tea, or water (control group) for 2 weeks. Electron paramagnetic resonance (EPR) spectroscopy was applied to measure the radical scavenging capacity of the skin in vivo before and after the intervention. Results: Both Yabukita and Benifuuki tea led to an increase in the radical scavenging activity of the skin by 28 and 29%, respectively, and the difference was significant when compared to the control group. Benifuuki tea, previously reported to be superior in bioavailability due to a highly absorbable methylated catechin, did not prove to be more effective than the common Yabukita tea. Conclusion: The results show that green tea enhances the radical scavenging capacity of the skin and support the hypothesis that green tea may offer protection against cutaneous oxidative stress.

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Topical lipophilic epigallocatechin-3-gallate on herpes labialis: a phase II clinical trial of AverTeaX formula

Cited by 15 publications

References 29 publications

Persistent Virucidal Activity in an Alcohol-Based Sanitizer Formulation (ProtecTeaV) for Potential Use against Norovirus

Persistent Virucidal Activity in an Alcohol-Based Sanitizer Formulation (ProtecTeaV) for Potential Use against Norovirus

Effect of Novel Formulations using Lipophilic Epigallocatechin-3-Gallate against Influenza Virus Infection

A Randomized Controlled Trial of Green Tea Beverages on the in vivo Radical Scavenging Activity in Human Skin

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