Topographic mapping of Helicobacter pylori colonization in long-term-infected pigs

Abstract

Four barrier-born pigs were inoculated with Helicobacterpylori during gastroscopy. Infection in all pigs was established after 3 weeks, and the animals were kept isolated from other pigs in ordinary experimental sties. The pigs were sacrificed and examined 3, 5, 6, and 6.5 months postinoculation. A detailed urease mapping of the pig stomachs showed a patchy distribution of H. pylon. The bacteria colonized in all pigs, with a concentration of H. pylon-positive areas in the antrum and fundus. Furthermore, the number of colonized areas tended to increase with time, and some of these areas showed a strong urease reaction, indicating a heavy colonization with H. pyloni. Biopsies from these areas contained 102 to 105 CFU per 2-mm-wide biopsy. We conclude that persistence ofH. pylon infection in barrier-born pigs can be demonstrated for at least 6.5 months.

Sensitivity of Helicobacter pylori detection by Giemsa staining is poor in comparison with immunohistochemistry and fluorescent in situ hybridization and strongly depends on inflammatory activity

“…Namely, H. pylori shows an uneven and patchy distribution on the gastric mucosa, especially in case of low bacterial density. 18,19 The consequences of this can be highlighted by analyzing the IHC-FISH discrepant cases of our study.…”

Quantitative study of Helicobacter pylori in gastric mucus by competitive PCR using synthetic DNA fragments

“…Both of these methods require biopsy samples, but not all biopsy samples contain a sufficient VOL. 34,1996 QUANTIFICATION OF H. PYLORI BY COMPETITIVE PCR 2423 amount of H. pylori organisms to allow detection, since the distribution of H. pylori in the stomach is patchy (8,19). Aspirated gastric juice contains gastric mucus in which H. pylori can be detected (16) and can usually be easily obtained in relatively large volumes.…”

pH-dependent binding ofHelicobacter pylorito pig gastric mucins

“…Mucins from the surface epithelium of the cardiac region, corpus and antrum were all shown to bind to H. pylori . Thus, no apparent selectivity was obtained towards mucins from a certain region of the gastric mucosa despite the regional distribution noticed in the inoculation experiments performed in the pig [6]. To investigate the binding of H. pylori towards gastric mucins, mucosal extracts separated by density gradient centrifugation were used.…”

“…Several attempts have been made to establish animal models for H. pylori infection. H. pylori strain SVA 40 has been successfully used in a pig model of H. pylori infection of the gastric mucosa [5] and a concentration of H. pylori ‐positive areas was found in antrum and fundus [6]. However, whether or not selective binding to mucins from certain regions in the stomach would explain this pattern of colonization has not been investigated.…”