Topography and pachymetry maps for mouse corneas using optical coherence tomography

Liu, Alice; Brown, Dillon; Conn, Rachel; McNabb, Ryan P.; Pardue, Machelle T.; Kuo, Anthony N.

doi:10.1016/j.exer.2019.107868

Cited by 9 publications

(4 citation statements)

References 25 publications

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“…Because there is no instrument available to analyze the topographical and pachymetric maps of small animals such as the mouse cornea, we used calibrated mouse corneal OCT scans and generated mouse anterior axial maps (corneal curvature) and pachymetric maps (corneal thickness) of control- and FGFR2cKO mice. 33 Figures 5 A and 5 B are representative images of the axial- and pachymetric maps of the corneas of control mice, respectively. Similarly, Figures 5 C and 5 D are representative images of corneal axial and pachymetric maps of FGFR2cKO mice, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Corneal topographers provide different maps to represent the measurements that characterize the surface of a KC cornea. Because of an absence of a commercially available mouse corneal topography analysis instrument, we used a Spectral Domain-OCT-based anterior curvature and thickness mapping analysis method as previously developed by Liu et al 33 Because the ANTERION OCT files could not be used for corneal topography mapping, we switched to the Spectral Domain-OCT method. Corneal topographical analysis program has been successfully used by other groups to determine corneal topography and pachymetry mapping in mouse models to study its relationship with KC.…”

Section: Discussionmentioning

confidence: 99%

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Role of Fibroblast Growth Factor Receptor 2 (FGFR2) in Corneal Stromal Thinning

Joseph,

Boateng,

Srivastava

et al. 2023

Invest. Ophthalmol. Vis. Sci.

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Purpose To determine the role of fibroblast growth factor receptor 2 (FGFR2)–mediated signaling in keratocytes during corneal development, a keratocyte-specific FGFR2-knockout (named FGFR2cKO ) mouse model was generated, and its phenotypic characteristics were determined. Methods A FGFR2cKO mouse model was generated by the following method: FGFR2 flox mice were crossed with the inducible keratocyte specific-Cre mice (Kera-rtTA/tet-O-Cre). Both male and female FGFR2cKO- and control mice (1 to 3-months-old) were analyzed for changes in corneal topography and pachymetry maps using the optical coherence tomography (OCT) method. The comparative TUNEL assay and immunohistochemical analyses were performed using corneas of FGFR2cKO and control mice to determine apoptotic cells, and expression of collagen-1 and fibronectin. Transmission electron microscopic analysis was conducted to determine collagen structures and their diameters in corneas of FGFR2cKO and control mice. Results OCT-analyses of corneas of FGFR2cKO mice (n = 24) showed localized central thinning and an increased corneal steepness compared to control mice (n = 23). FGFR2cKO mice further showed a decreased expression in collagen-1, decreased collagen diameters, acute corneal hydrops, an increased fibronectin expression, and an increased number of TUNEL-positive cells suggesting altered collagen structures and keratocytes’ apoptosis in the corneas of FGFR2cKO mice compared to control mice. Conclusions The FGFR2cKO mice showed several corneal phenotypes (as described above in the results) that are also exhibited by the human keratoconus corneas. The results suggested that the FGFR2cKO mouse model serves to elucidate not only the yet unknown role of FGFR2-mediated signaling in corneal physiology but also serves as a model to determine molecular mechanism of human keratoconus development.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Role of Fibroblast Growth Factor Receptor 2 (FGFR2) in Corneal Stromal Thinning

Joseph,

Boateng,

Srivastava

et al. 2023

Invest. Ophthalmol. Vis. Sci.

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“…In summary, OCT can provide important clinical information and was shown to be useful to date. As was also shown in appropriate animal models, the device is useful in basic research [ 72 , 73 , 108 , 109 , 110 ]. Such new technological achievements will certainly complement the complex culture experiments in the future.…”

Section: Development Of 3d Cell Culture Modelsmentioning

confidence: 99%

“…Previously, these were not or insufficiently well studied, and it is important to explore this ultrastructural part of the eye in more detail (reviewed in [ 64 , 65 ]) [ 66 , 67 , 68 , 69 , 70 ]. As described below in more detail, optical coherence tomography (OCT) is an important technique in visualizing also the ultrastructure of the cornea [ 71 , 72 ]. Exemplarily, Napoli and colleagues have used the OCT to explore the corneal stromal striae in Ovis aries [ 73 ].…”

Section: Development Of 3d Cell Culture Modelsmentioning

confidence: 99%

The Communication between Ocular Surface and Nasal Epithelia in 3D Cell Culture Technology for Translational Research: A Narrative Review

Aydin

Dietrich

Witt

et al. 2021

IJMS

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There is a lack of knowledge regarding the connection between the ocular and nasal epithelia. This narrative review focuses on conjunctival, corneal, ultrastructural corneal stroma, and nasal epithelia as well as an introduction into their interconnections. We describe in detail the morphology and physiology of the ocular surface, the nasolacrimal ducts, and the nasal cavity. This knowledge provides a basis for functional studies and the development of relevant cell culture models that can be used to investigate the pathogenesis of diseases related to these complex structures. Moreover, we also provide a state-of-the-art overview regarding the development of 3D culture models, which allow for addressing research questions in models resembling the in vivo situation. In particular, we give an overview of the current developments of corneal 3D and organoid models, as well as 3D cell culture models of epithelia with goblet cells (conjunctiva and nasal cavity). The benefits and shortcomings of these cell culture models are discussed. As examples for pathogens related to ocular and nasal epithelia, we discuss infections caused by adenovirus and measles virus. In addition to pathogens, also external triggers such as allergens can cause rhinoconjunctivitis. These diseases exemplify the interconnections between the ocular surface and nasal epithelia in a molecular and clinical context. With a final translational section on optical coherence tomography (OCT), we provide an overview about the applicability of this technique in basic research and clinical ophthalmology. The techniques presented herein will be instrumental in further elucidating the functional interrelations and crosstalk between ocular and nasal epithelia.

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