Total and differential bulk cow milk somatic cell counts and their relation with lipolysis

Gargouri, Ali; Hamed, Houda; Elfeki, Abdelfettah

doi:10.1016/j.livsci.2007.05.007

Cited by 32 publications

(28 citation statements)

References 30 publications

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“…One of the most ideal biomarkers in mastitis is milk leukocyte count. In the present study, a higher level of milk leukocytes is indicative of the immune response caused by the microorganisms in the mastitis affected mammary gland (Djabri et al, 2002;Gargouri et al, 2008). These results are in line with the findings of Hussain et al, 2012 andHussain et al, 2013.…”

Section: Resultssupporting

confidence: 93%

Haemato-biochemical profile and milk leukocyte count in subclinical and clinical mastitis affected crossbred cattle

K¹,

Satyanarayana²,

Narayanaswamy³

et al. 2017

JEBAS

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The study was conducted to determine the effect of subclinical mastitis (SCM) and clinical mastitis (CM) on haemato-biochemical parameters and milk leukocyte count of crossbred cattle. Milk and blood samples were collected from 20 healthy, 223 SCM and 47 CM affected animals from Kolar and Chikkaballapur districts of Karnataka state, India. The SCM were diagnosed by California Mastitis Test (CMT) and electrical conductivity (EC) of milk. Blood and milk of mastitis infected crossbred cattle were analyzed for haemato-biochemical parameters and milk leukocyte count. Haematology showed significant increase in TLC in crossbred cows affected with mastitis. Significantly (P< 0.05) lower average values of TEC, Hb and PCV were observed in SCM infected animals, however no significant change were observed in values of CM infected than healthy animals. Differential leucocytic count (DLC) revealed higher granulocytes and lymphopenia in mastitis infected animals. Biochemical estimation revealed significantly (P< 0.05) higher average values of Ca, P, Na, Cl, and K in mastitis infected compared with healthy animals however, no significant (P> 0.05) change was observed in Mg and ALT levels. AST and TP values were significantly increased in SCM infected compared to healthy animals however, no significant change were observed in CM infected animals.

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Section: Resultssupporting

confidence: 93%

Haemato-biochemical profile and milk leukocyte count in subclinical and clinical mastitis affected crossbred cattle

K¹,

Satyanarayana²,

Narayanaswamy³

et al. 2017

JEBAS

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“…The cell populations in milk DLC were in agreement with the previous reports (Mukherjee & Dang 2011;Mukherjee et al 2013). On comparing the result of milk DLC, it was found that PMN and macrophage percentage were lower and lymphocyte percentages were higher in milk samples which are in agreement to the reports of Mukherjee and Dang (2011); Gargouri et al (2008). They reported that lymphocytes were the predominant cell types, but their proportion declined with total bulk milk SCC.…”

Section: Discussionsupporting

confidence: 91%

Seasonal variation inin vitroimmune activity of milk leukocytes in elite and non-elite crossbred cows of Indian sub-tropical semi-arid climate

Mukherjee¹,

Chaudhury

et al. 2015

Biological Rhythm Research

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Immunity of mammary gland in terms of in vitro activity of milk leukocytes has been evaluated during hot-humid, summer, and winter season in elite (n = 10) and non-elite (n = 10) crossbred cows. Milk samples were collected from all the cows throughout the year at 15-day interval. Milk somatic cell counts (SCC) and differential leukocyte counts (DLC) were evaluated microscopically. Milk neutrophils, macrophages, and lymphocytes were isolated and cultured in vitro. In vitro PI of milk neutrophils and macrophages was evaluated by colorimetric NBT (nitro-blue tetrazolium) reductive assay. Mitogen-induced milk lymphocyte blastogenic response was measured by colorimetric MTT (thiazolyl blue tetrazolium bromide) assay. Milk SCC was found to be significantly (p < 0.01) higher in elite cows compared to non-elite cows irrespective of season. There was significant (p < 0.05) increase in milk SCC during hot-humid season compared to winter season in both the group of the cows. There was no significant difference between group and season in terms of DLC. In vitro phagocytic index of elite cows was significantly (p < 0.01) higher than non-elite cows. The phagocytic index was significantly (p < 0.01) decreased in summer and hot-humid season compared to winter season in both the group of animals. Macrophages isolated from elite cows having significantly (p < 0.01) lower phagocytic index than non-elite cows which significantly (p < 0.01) decreased during summer and hot-humid season compared to winter. In vitro milk lymphocyte proliferative response was significantly (p < 0.01) lower in elite cows. Activity of B-lymphocytes decreased significantly (p < 0.01) during summer and hot-humid season than winter, but activity of T-lymphocytes remains unaltered during different seasons. In conclusion, the mammary immunity in terms of in vitro activity of milk leukocytes is compromised during summer and hot-humid season in elite crossbred cows; therefore, better care and management should be taken in high-yielding cows during summer and hot-humid season to minimize intramammary infections.

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“…A high level of lipolysis is actually associated with a high level of PMN but the mechanism by which PMN affect fat lipolysis remains to be determined (Gargouri et al 2008). Milk SCC are suspected to be involved in the lipolysis of the fat globule triglycerides and thus in off-flavor development due to free fatty acids (Azzara and Dimick 1985a;Gargouri et al 2008;Ma et al 2000;Murphy et al 1989;Santos et al 2003). Moreover, lipases can resist pasteurization (Ma et al 2000;Shipe and Senyk 1981) and high SCC pasteurized dairy products are thus more likely to develop off-flavors than low SCC ones.…”

Section: Elastasementioning

confidence: 99%

Mastitis impact on technological properties of milk and quality of milk products—a review

Maréchal

Thiéry²,

Vautor

et al. 2011

Dairy Science & Technol.

150

139

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The consequences of mastitis on the technological properties of milk and on the quality of milk products are widely reported in the literature. Besides, recent advances have shed light on the mechanisms involved in the udder response and subsequent milk changes in mastitis cases. This review gives an update on the literature regarding the impact of mastitis on milk composition and processing properties and collates recent data regarding the mechanisms involved in mastitis effects. It is an attempt to link field observations and experimental studies in order to better understand how mastites affect so dramatically the technological properties of milk. Both bovine and small ruminant milks are considered and a special emphasis is given on the role of staphylococci, streptococci, and Escherichia coli, the most common causative agents of mastitis.

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Total and differential bulk cow milk somatic cell counts and their relation with lipolysis

Cited by 32 publications

References 30 publications

Haemato-biochemical profile and milk leukocyte count in subclinical and clinical mastitis affected crossbred cattle

Haemato-biochemical profile and milk leukocyte count in subclinical and clinical mastitis affected crossbred cattle

Seasonal variation inin vitroimmune activity of milk leukocytes in elite and non-elite crossbred cows of Indian sub-tropical semi-arid climate

Mastitis impact on technological properties of milk and quality of milk products—a review

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