Total polyphenols and antioxidant activities of green tea powder from GMB 7 and GMB 9 tea clones

Abstract

People’s interest in green tea powder is increasing currently. The addition of green tea powder to food products could increase antioxidant activity and other health benefits. GMB 7 and GMB 9 are local tea clones of the Assam variety widely planted in Indonesia’s tea plantation. This study aimed to determine green tea powder’s total polyphenols and antioxidant activity from local tea clones (GMB 7 and GMB 9). Total polyphenol and antioxidant activity were determined by Folin-Ciocalteau and free radical of DPPH methods, respectively. Our results showed that the total polyphenols of green tea powder were 27.61% and 27.31% for the GMB 7 and GMB 9 clones, respectively. Furthermore, the antioxidant activity of green tea powder with IC50 was 15.41 mg/L and 17.32 mg/L for clones GMB 7 and GMB 9, respectively. The results indicate the potential for the development and utilization of local clones to fulfill domestic green tea powder production. Further research is needed to determine the health benefits of green tea powder from clones of The Assam varieties.

Perubahan Pigmen Klorofil dan Karotenoid serta Warna Bubuk Teh Hijau dari Berbagai Klon pada Kondisi Penyimpanan Berbeda

“…Ketertarikan konsumen terhadap bubuk teh hijau (green tea powder atau GTP) terus meningkat memiliki manfaat dan mudah diaplikasikan untuk proses ekstraksi maupun produk pangan (Komes et al, 2010;Trinovani et al, 2022). Bubuk teh hijau memiliki aktivitas antioksidan yang tinggi dan mengandung sebagian besar katekin dari daun teh segar (Karori et al, 2007;Yang dan Landau, 2000).…”

“…Daun teh kering dihancurkan sebelum digunakan untuk produksi bubuk teh hijau. Bubuk teh hijau diproduksi menggunakan millstone grinder (Indonesia) dengan kecepatan 25 rpm hingga dihasilkan bubuk teh hijau dengan ukuran partikel 1,58 µm sebagaimana pada penelitian sebelumnya (Trinovani et al, 2022). Setiap perlakuan klon dilakukan 3 kali ulangan proses produksi.…”

Effect of fixation methods and various clones of Camellia sinensis var. sinensis (L) properties and antioxidant activity of Indonesian green tea

“…The determination of polyphenols in tea using the Folin-Ciocalteu method refers to ISO 14502-1:2005 (Trinovani et al, 2022). A total of 1 mL of green tea extract solution was pipetted into a 50 mL volumetric flask and diluted with distillation water.…”

“…The antioxidant activity of green tea was measured using the DPPH assay (Trinovani et al, 2022). Green tea extract was diluted at different concentrations: 10, 15, 20, and 25 ppm in 70% methanol.…”

<i>In Vitro</i> Antioxidant and Free Radical Scavenging Activity of <i>Curcuma longa</i>, <i>Acorus calamus and Camellia sinensis</i>

“…Also, from a health perspective, adding tea powder to baked goods lowers the glycemic index. Green tea powder also contains a lot of vitamin C [14] [15] [16] [17]. Therefore, the purpose of the current study was to determine the total phenolic and flavonoid content and total antioxidant activity of the Curcuma longa, Acorus calamus and Camellia sinensis ethanolic extracts and their free radical scavenging activity.…”