Toward production of jet fuel functionality in oilseeds: identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in<i>Camelina</i>seeds

Kim, Hae Jin; Silva, Jillian E.; Vu, Hieu; Mockaitis, Keithanne; Nam, Jeong-Won; Cahoon, Edgar B.

doi:10.1093/jxb/erv225

Cited by 75 publications

(109 citation statements)

References 63 publications

Supporting

Mentioning

106

Contrasting

Order By: Relevance

“…The previously described transcriptome of developing C. avigera var pulcherrima seeds (Kim et al, 2015b) was mined for candidate genes encoding DGATs that are specialized for medium-chain fatty acid metabolism. Sequences for one DGAT1-like gene designated CpuDGAT1 and three DGAT2-like genes designated Figure 1.…”

Section: Resultsmentioning

confidence: 99%

“…Seed-specific CpuDGAT1 Overexpression Enhances 10:0 Content in Camelina Seeds CpuDGAT1 was further examined for its ability to enhance 8:0 and 10:0 accumulation in camelina seeds resulting from expression of CvFatB1 encoding a specialized Cuphea thioesterase (Kim et al, 2015b). We also examined the ability of CpuDGAT1 to increase the accumulation of these fatty acids when coexpressed with the previously described CvLPAT2 that encodes an LPAT specialized for in planta accumulation of 8:0 and 10:0 (Kim et al, 2015a).…”

Section: Functional Characterization Of Cpudgat1mentioning

confidence: 99%

“…Seed oils of Cuphea species such as C. avigera var pulcherrima and C. viscosissima accumulate the medium-chain length fatty acids octanoic (8:0) and decanoic (10:0) acids to .90 mol % of TAG fatty acids (Graham, 1989;Kim et al, 2015b). By contrast, seed oils of most plants, including those of the major commercial oilseed crops, are enriched in fatty acids with 16-and 18-carbon atoms.…”

mentioning

confidence: 99%

See 2 more Smart Citations

A Specialized Diacylglycerol Acyltransferase Contributes to the Extreme Medium-Chain Fatty Acid Content of Cuphea Seed Oil

et al. 2017

Self Cite

View full text Add to dashboard Cite

Seed oils of many Cuphea sp. contain .90% of medium-chain fatty acids, such as decanoic acid (10:0). These seed oils, which are among the most compositionally variant in the plant kingdom, arise from specialized fatty acid biosynthetic enzymes and specialized acyltransferases. These include lysophosphatidic acid acyltransferases (LPAT) and diacylglycerol acyltransferases (DGAT) that are required for successive acylation of medium-chain fatty acids in the sn-2 and sn-3 positions of seed triacylglycerols (TAGs). Here we report the identification of a cDNA for a DGAT1-type enzyme, designated CpuDGAT1, from the transcriptome of C. avigera var pulcherrima developing seeds. Microsomes of camelina (Camelina sativa) seeds engineered for CpuDGAT1 expression displayed DGAT activity with 10:0-CoA and the diacylglycerol didecanoyl, that was approximately 4-fold higher than that in camelina seed microsomes lacking CpuDGAT1. In addition, coexpression in camelina seeds of CpuDGAT1 with a C. viscosissima FatB thioesterase (CvFatB1) that generates 10:0 resulted in TAGs with nearly 15 mol % of 10:0. More strikingly, expression of CpuDGAT1 and CvFatB1 with the previously described CvLPAT2, a 10:0-CoA-specific Cuphea LPAT, increased 10:0 amounts to 25 mol % in camelina seed TAG. These TAGs contained up to 40 mol % 10:0 in the sn-2 position, nearly double the amounts obtained from coexpression of CvFatB1 and CvLPAT2 alone. Although enriched in diacylglycerol, 10:0 was not detected in phosphatidylcholine in these seeds. These findings are consistent with channeling of 10:0 into TAG through the combined activities of specialized LPAT and DGAT activities and demonstrate the biotechnological use of these enzymes to generate 10:0-rich seed oils.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Functional Characterization Of Cpudgat1mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

A Specialized Diacylglycerol Acyltransferase Contributes to the Extreme Medium-Chain Fatty Acid Content of Cuphea Seed Oil

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…C16:0-ACP is hydrolyzed by FatB thioesterases, while FatA is more specific to C18:0-and C18:1-ACP. Cuphea species that accumulate MCFA in their TAG have specific FatB genes (Kim et al, 2015b). Expression of different Cuphea FatB genes associated with different MCFA profiles (C8, C10, C16) led to significant MCFA accumulation in camelina seeds.…”

Section: Developing New Camelina Oil Profiles For Industrymentioning

confidence: 99%

“…Expression of different Cuphea FatB genes associated with different MCFA profiles (C8, C10, C16) led to significant MCFA accumulation in camelina seeds. This effect was enhanced by the coexpression of coconut lysophosphatidic acid acyltransferase (LPAT) and the inhibition of the endogenous camelina plastidial beta-ketoacyl-ACP synthase CsKASII (Kim et al, 2015b).…”

Section: Developing New Camelina Oil Profiles For Industrymentioning

confidence: 99%

Camelina, a Swiss knife for plant lipid biotechnology

Faure

Tepfer

2016

OCL

View full text Add to dashboard Cite

-Camelina has emerged in the last decade as a multipurpose crop plant particularly suitable for engineering new lipids for diverse uses, including feed, biofuel and green chemistry. The rebirth of this ancient crop was based on several intrinsic favorable characteristics: robust agronomic qualities, attractive oil profile, genetic proximity with the model plant arabidopsis, ease of genetic transformation by floral dip. The need to increase both the production and diversity of plant oils, while improving the sustainability of agricultural systems, has been the driving forces behind the ever-increasing investment in camelina research. Worldwide interest in engineering camelina has led to the development of a remarkable pipeline that allows the rapid production and phenotyping of new lines; it includes specific tools, such as databases, collections of natural accessions, methods of genetic transformation and lipid analysis. Implementation of numerous metabolic pathways in camelina for the production of novel lipids has highlighted the potential as well as the versatility of this new "old" oilseed crop that is well on the way to becoming an ideal plant chassis for lipid synthetic biology.Keywords: Lipid metabolism / oilseed / omega-3 / biofuels Résumé -La cameline, véritable couteau-suisse pour la biotechnologie végétale des lipides. La caméline a émergé durant la dernière décennie comme une espèce agronomique polyvalente particulièrement adaptée à l'ingé-nierie des lipides pour divers usages comme les biocarburants, l'alimentation ou la chimie verte. La renaissance de cette espèce cultivée ancienne est fondée sur plusieurs caractéristiques : robustesse agronomique, profil en huile attractif, proximité génétique avec la plante modèle arabidopsis et très grande facilité de transformation génétique par infiltration des fleurs. Le besoin d'augmenter la production d'huile végétale tout en diversifiant la nature des lipides produits, associé à l'amélioration de la durabilité des systèmes de production, ont été les déterminants qui ont poussé le développement de la recherche autour de la caméline. L'intérêt de par le monde pour l'ingénierie des lipides chez la caméline a ainsi permis la mise en place d'un ensemble d'outils incluant des bases de données, des collections d'accessions naturelles, du matériel spécifique pour la transformation et le phénotypage, ainsi que des méthodes pour la production et la caractérisation de nouvelles variétés ou de lignées. Les nombreux exemples de mise en place de nouvelles voies métaboliques chez la caméline pour la production de lipides spécifiques illustrent le potentiel important de cette nouvelle « ancienne » espèce oleogineuse qui est en passe de devenir un chassis végétal idéal pour la biologie synthétique des lipides. Mots clés :Métabolisme lipidique / oléagineux / oméga-3 / biocarburants

show abstract

Transgenic Oils

Durrett¹

2020

Bailey's Industrial Oil and Fat Products

View full text Add to dashboard Cite

Genetic engineering (GE) technology offers a powerful tool for developing a broad range of oils with specialized compositions useful for different food and industrial applications. Such oils could contain modified ratios of endogenous fatty acids, or even unusual fatty acids and lipids, produced through the transgenic expression of biosynthetic genes from other species. However, GE technology has mostly been limited to crop input traits such as herbicide tolerance and insect resistance. As this article will discuss, only recently have high oleic acid soybeans with transgenically modified oil compositions been grown on a commercial basis. For the most part, the synthesis of unusual fatty acids with useful functional groups only results in the accumulation of low levels of the desired product in the transgenic seed. The continually improving knowledge of fatty acid biosynthesis and triacylglycerol assembly in oilseeds will be explored. This knowledge, combined with new systems and synthetic biology approaches, promises to overcome biological bottlenecks limiting the production of unusual fatty acids in transgenic oilseeds. This article will also describe the regulatory and economic barriers that likely present significant barriers to the widespread adoption of novel oils with altered output traits. As discussed, new business models for the commercial production of specialized industrial oils will therefore need to be considered.

show abstract

Toward production of jet fuel functionality in oilseeds: identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies inCamelinaseeds

Abstract: HighlightTranscriptomic data mining and combinatorial gene stacking was used for metabolic engineering of Camelina to produce oils containing medium-chain length fatty acids that mimic jet fuel hydrocarbon components.

Cited by 75 publications

References 63 publications

A Specialized Diacylglycerol Acyltransferase Contributes to the Extreme Medium-Chain Fatty Acid Content of Cuphea Seed Oil

A Specialized Diacylglycerol Acyltransferase Contributes to the Extreme Medium-Chain Fatty Acid Content of Cuphea Seed Oil

Camelina, a Swiss knife for plant lipid biotechnology

Transgenic Oils

Contact Info

Product

Resources

About