Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: I. Analyte definition and proposal of a candidate reference method

Jeppsson, Jan‐Olof; Arndt, T.; Schellenberg, François; Wielders, Jos P.M.; Anton, Raymond F.; Whitfield, John B.; Helander, Anders

doi:10.1515/cclm.2007.107

Cited by 106 publications

(54 citation statements)

References 25 publications

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“…This prompted the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) to initiate a Working Group aiming for standardization of CDT measurements (WG-CDT). In its first publication [ 7 ], disialotransferrin (disialylated monoglycan transferrin) was defined as the measurand for a reference method and the target analyte for the standardization work, and high-performance liquid chromatography (HPLC) [ 8 ] with photometric detection was proposed as the candidate reference method. A network of reference laboratories running this method was formed demonstrating good intra-and inter-laboratory performance [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: III. Performance of native serum and serum spiked with disialotransferrin proves that harmonization of CDT assays is possible

Weykamp

Wielders

Helander

et al. 2013

Clinical Chemistry and Laboratory Medicine

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Carbohydrate-deficient transferrin (CDT) is a generic term that refers to the transferrin glycoforms whose concentration in blood is temporarily increased by sustained alcohol consumption. Due to high clinical specificity, CDT was proposed as a biomarker of heavy alcohol use and has been available for about 20 years. A number of methods have been developed for CDT measurement based on different analytical techniques and principles and without any harmonization or calibration to a reference method. As a consequence, neither the reference limits nor the cut-off values have been similar across assays, hampering understanding of the diagnostic value of CDT and its routine use. This prompted the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) to initiate a Working Group on Standardization of CDT (WG-CDT). This third publication of the WG-CDT is devoted to testing the commutability of native and disialotransferrin-spiked serum panels as candidate secondary reference materials, in order to prove the harmonization potential of commercial CDT methods. The results showed that assay harmonization reduced the inter-laboratory imprecision in a network of reference laboratories running the HPLC candidate reference method. In the seven commercial methods evaluated in this study, the use of multi-level secondary calibrators of human serum origin significantly reduced the between-method imprecision. Thus, harmonization of CDT measurements by different methods can be achieved using this calibration system, opening the way for a full standardization of commercial methods against a reference method by use of certified reference materials.

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Section: Introductionmentioning

confidence: 99%

Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: III. Performance of native serum and serum spiked with disialotransferrin proves that harmonization of CDT assays is possible

Weykamp

Wielders

Helander

et al. 2013

Clinical Chemistry and Laboratory Medicine

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“…These actions would improve the diagnostic characteristics of CDT as a biomarker for chronic alcohol consumption and promote comparability between laboratories. As a result of these measures, the quality of cooperation between laboratory and court medicine would be improved, along with medical diagnostics and patient care (12,13,25,26).…”

Section: Discussionmentioning

confidence: 99%

Carbohydrate-Deficient Transferrin - A Contemporary Biomarker in Comparison with Traditional Laboratory Markers of Chronic Alcohol Abuse

Maksić¹,

Vodnik²,

Stanković³

et al. 2010

Journal of Medical Biochemistry

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Summary: Timely identification of excessive alcohol use and its potential complications is a prerequisite for successful treatment. Several routine tests have been used in laboratories that may help in diagnosing alcoholism, such as determination of MCV, AST, ALT, GGT, but it has been shown that they lack specificity and sensitivity. Contemporary biomarkers are increasingly being used today that may due to their unique characteristics help in disco vering the onset of chronic alcohol abuse, as well as in abstinence and relapse monitoring. The term carbohydrate-deficient transferrin (CDT) stands for a small group of human transferrin isoforms (asialo, monosialo, and disialotran sferrin) with a lower degree of glycosylation in comparison to the dominant transferrin isoform (tetrasialotransferrin). Persons consuming large quantities of alcohol (≥50 -80 g daily) over a period of at least two weeks have increased concentrations of transferrin isoforms lacking one (disialo transferrin) or both (asialotransferrin) carbohydrate chains. In this paper the traditional markers of chronic alcohol abuse (GGT, AST, ALT, and MCV) were determined, as well as the new biomarker CDT, after which diagnostic evaluation was performed and their usability and clinical value in routine laboratory practice were estimated. These markers were also determined in heavy alcoholics on admission into hospital and after two weeks of therapy, with the aim of estimating their diagnostic value for abstinence and relapse monitoring. Keywords: alcoholism, biomarkers, CDT, transferrinKratak sadr`aj: Pravovremeno prepoznavanje preko merne upotrebe alkohola i potencijalnih komplika cija pred uslov je za uspe{no le~enje. U laboratorijama se koristi neko liko rutinskih testova koji mogu da pomognu u dijagnostici alkoholizma, kao npr. odre|ivanje MCV, AST, ALT, GGT, ali su se pokazali kao nedovoljno specifi~ni i osetljivi. Danas su sve vi{e u upotrebi savremeni biomarkeri koji zahvaljuju}i svojim jedin stvenim osobinama mogu da pomognu kod otkrivanja po ja ve hroni~nog alkoholizma, kao i pri pra}enju apstinencije i relapsa. Naziv transferin deficijentan ugljenim hidratima (CDT) podrazumeva manju grupu izoformi humanog trans ferina (asialo-, monosialo-i disialo-transferin), koje imaju ni`i stepen glikozilacije u pore|enju sa do minantnom izo for mom transferina (tetrasialotran sfe rin). Osobe koje unose velike koli~ine alkohola (≥50-80 g dnevno) u periodu od najmanje 2 nedelje imaju povi{ene koncentracije izoformi transferina kojima nedostaje jedan (disialotransferin) ili oba (asialotransferin) ugljeno hidratna lanca. U ovom radu oba vlje no je odre|ivanje tradicionalnih marke ra hroni~ne alko ho lne potro{nje (GGT, AST, ALT i MCV), kao i novog bio markera CDT, a zatim je izvr{ena dijagno sti~ka evaluacija i procena njihove upotrebljivosti i klini~ke vrednosti u rutin skoj laboratorijskoj praksi. Tako|e je izvr{eno odre|ivanje navedenih markera kod te{kih alkoholi~ara pri prijemu na bolni~ko odeljenje i nakon 2 nedelje terapije u cilju proce ne njihove dijag...

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“…For instance, differen tial diagnosis of infectious mononucleosis and serum sickness from the healthy state is based on the reactivity of heterophil antibodies to specific viral carbohydrate antigens, Paul-Bunnell antigen, N-glycolylneuraminic acid and Forsmann antigen (57-59). Well known examples are also glycosylated hemoglobin in monitoring diabetic patients (63), or abnormally glycosylated bands of erythrocyte membranes, as well as carbohydrate-deficient transferrin for diagnosis of inherited or acquired disorders of glycosylation and alcohol abuse (64,65).…”

Section: Carbohydrates In Therapy and Diagnosticsmentioning

confidence: 99%

Glycans as Biomarkers: Status and Perspectives

Janković¹

2011

Journal of Medical Biochemistry

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lanaca koji su kovalentno vezani za poli pep tidnu ki~mu. Dobro je poznat zna~aj prome na u glikozilaciji proteina za nastanak, razvoj i krajnji ishod razli~itih bolesti kod ljudi. Glikani se smatraju jedinstvenim strukturama za di jag nozu, i pra}enje toka bolesti. U »omics« eri, glikom, glika n ski analog proteoma i genoma, predstavlja mogu}i izvor no vih biomarkera. Kreiranje strategije za otkri}e biomarkera zahteva nove principe i platforme za analizu relativno malih koli~ina brojnih glikoproteina. O~ekuje se da glikomske teh no logije, koje su jo{ u razvoju, a koje obuhvataju razli~ite tipove masene spektrometrije i afinitivnih tehnika, rezultiraju novim analiti~kim procedurama u oblasti odre |i vanja bio markera. Najve}i izazovi za eksperimentalnu i kli ni~ku gliko pro teomiku su: pretraga razli~itih tipova gliko mo lekula, oda bir potencijalnih markera i njihova selekcija ili pre ~i{}a -vanje i identifikacija. Za ovo je neophodno razviti teh no logije koje }e omogu}iti visoku senzitivnost detekcije biomarkera kao i odgovaraju}u standardizaciju i validaciju novih metoda, kako bi se one mogle primeniti u laboratorijskom radu. Dalji razvoj na polju primenjene glikonauke zahteva integrisani sistemski pristup sa ciljem da se na pravi na~in iskoriste sve njene mo gu}nosti u dijagnostici.

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Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: I. Analyte definition and proposal of a candidate reference method

Cited by 106 publications

References 25 publications

Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: III. Performance of native serum and serum spiked with disialotransferrin proves that harmonization of CDT assays is possible

Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: III. Performance of native serum and serum spiked with disialotransferrin proves that harmonization of CDT assays is possible

Carbohydrate-Deficient Transferrin - A Contemporary Biomarker in Comparison with Traditional Laboratory Markers of Chronic Alcohol Abuse

Glycans as Biomarkers: Status and Perspectives

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