Toward the prediction of PSE-like muscle defect in hams: Using chemometrics for the spectral fingerprinting of plasma

Théron, Laëtitia; Sayd, Thierry; Chambon, Christophe; Vautier, Antoine; Ferreira, Claude; Aubry, Laurent; Ferraro, Vincenza; Santé-Lhoutellier, Véronique

doi:10.1016/j.foodcont.2019.106929

Cited by 11 publications

(12 citation statements)

References 18 publications

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“…For instance, to predict pale soft exudative-like defects in cooked hams, Theŕon et al developed a predictive method to classify raw material prior to ham processing using blood samples, by studying the spectral data of a proteomics analysis of plasma. 94 Spectral fingerprints of proteins and their secondary structures were obtained using MALDI-TOF-MS and Attenuated Total Reflectance -Fourier Transform Infrared (ATR-FTIR) spectroscopy, respectively. Another proteomics approach was proposed by Zhu et al, who were looking for biomarkers of tenderness on Irish cattle, based on nano-LC-Q-Orbitrap-MS analysis.…”

Section: T H Imentioning

confidence: 99%

Foodomics: Analytical Opportunities and Challenges

et al. 2021

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Section: T H Imentioning

confidence: 99%

Foodomics: Analytical Opportunities and Challenges

et al. 2021

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“…The liver protein fingerprints were acquired according to [10]. For MALDI-TOF acquisition, 1 µL of pre-purified plasma proteins was manually spotted in triplicate on a polished steel target (MTP 384 Target Plate Polished Steel, Bruker Daltonics GmbH, Bremen, Germany).…”

Section: Maldi-tof Mass Spectrometry Protein Fingerprintingmentioning

confidence: 99%

“…As such, they constituted a potential fraud detection, since this product has a Protected Designation of Origin label legislating this aspect of production. More recently, the protein fingerprint successfully predicted the occurrence of normal and PSE-like defect in pork muscle based on a spectral fingerprint of plasma protein samples [10].…”

Section: Introductionmentioning

confidence: 99%

Detection of Frozen–Thawed Duck Fatty Liver by MALDI-TOF Mass Spectrometry: A Chemometrics Study

et al. 2021

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The marketing of poultry livers is only authorized as fresh, frozen, or deep-frozen. The higher consumer demand for these products for a short period of time may lead to the marketing of frozen–thawed poultry livers: this constitutes fraud. The aim of this study was to design a method for distinguishing frozen–thawed livers from fresh livers. For this, the spectral fingerprint of liver proteins was acquired using Matrix-Assisted Laser Dissociation Ionization-Time-Of-Flight mass spectrometry. The spectra were analyzed using the chemometrics approach. First, principal component analysis studied the expected variability of commercial conditions before and after freezing–thawing. Then, the discriminant power of spectral fingerprint of liver proteins was assessed using supervised model generation. The combined approach of mass spectrometry and chemometrics successfully described the evolution of protein profile during storage time, before and after freezing-thawing, and successfully discriminated the fresh and frozen–thawed livers. These results are promising in terms of fraud detection, providing an opportunity for implementation of a reference method for agencies to fight fraud.

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“…In the case of PSE meat, changes in the sarcoplasmic and myofibrillar proteins are observed, and specifically, a lower solubility and higher denaturation of the proteins are evidenced [ 8 ]. This phenomenon could be the result of several biological pathways such as fast post-mortem glycolysis in the muscle, which are specifically targeted by activation of glycogen phosphorylase and phosphofructokinase, reducing the pH and protein denaturation and therefore inducing the PSE defect [ 9 , 10 ]. Consequently, pig stress has adverse consequences on fresh pork due to the impact on the transformation of muscle into meat.…”

Section: Introductionmentioning

confidence: 99%

“…Considering the above, this is a major problem to the industry due to the economic losses, as this type of meat cannot be used for the elaboration of high-value cured meat products and other products. In this sense, a prediction of PSE through protein modifications would be a powerful strategy [ 11 ], among other emerging chemometric methods [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Proteomic Technologies to Assess the Quality of Raw Pork and Pork Products: An Overview from Farm-To-Fork

López‐Pedrouso

Lorenzo

Gagaoua

et al. 2020

Biology

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The quality assurance of pork meat and products includes the study of factors prior to slaughter such as handling practices, diet and castration, and others during the post-mortem period such as aging, storage, and cooking. The development over the last two decades of high-throughput techniques such as proteomics offer great opportunities to examine the molecular mechanisms and study a priori the proteins in the living pigs and main post-mortem changes and post-translational modifications during the conversion of the muscle into the meat. When the most traditional crossbreeding and rearing strategies to improve pork quality were assessed, the main findings indicate that metabolic pathways early post-mortem were affected. Among the factors, it is well documented that pre-slaughter stress provokes substantial changes in the pork proteome that led to defective meat, and consequently, novel protein biomarkers should be identified and validated. Additionally, modifications in pork proteins had a strong effect on the sensory attributes due to the impact of processing, either physical or chemical. Maillard compounds and protein oxidation should be monitored in order to control proteolysis and volatile compounds. Beyond this, the search of bioactive peptides is becoming a paramount goal of the food and nutraceutical industry. In this regard, peptidomics is a major tool to identify and quantify these peptides with beneficial effects for human health.

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Toward the prediction of PSE-like muscle defect in hams: Using chemometrics for the spectral fingerprinting of plasma

Cited by 11 publications

References 18 publications

Foodomics: Analytical Opportunities and Challenges

Foodomics: Analytical Opportunities and Challenges

Detection of Frozen–Thawed Duck Fatty Liver by MALDI-TOF Mass Spectrometry: A Chemometrics Study

Application of Proteomic Technologies to Assess the Quality of Raw Pork and Pork Products: An Overview from Farm-To-Fork

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