Toward understanding the ionization of biomarkers from micrometer particles by bio-aerosol mass spectrometry

Russell, Scott C.; Czerwieniec, Gregg; Lebrilla, Carlito B.; Tobias, Herbert J.; Fergenson, David P.; Steele, Paul T.; Pitesky, Maurice; Horn, Joseph M.; Srivastava, Abneesh; Frank, Matthias; Gard, Eric E.

doi:10.1016/j.jasms.2004.02.013

Cited by 33 publications

(26 citation statements)

References 26 publications

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“…This is the first time a potentially unique biomarker was measured in M. tuberculosis H37Ra at the single-cell and single-particle level in real time. A biological particle detector that could detect and identify various airborne bacterial spores (8,34,41), vegetative cells, viruses, and biological toxins is the overall goal of these studies. Below, we present some of the improvements we are currently developing in order for BAMS to mature towards a detector that is useful in more realistic environmental backgrounds expected in a multitude of diagnostic scenarios, such as dirty air and respiratory effluent.…”

Section: Resultsmentioning

confidence: 99%

“…An aerosol time-of-flight mass spectrometer (model 3800; TSI) based on a transportable design (11) was used with modifications, as previously described in detail (8,34,41) and referred to here as BAMS (Fig. 1A).…”

Section: Methodsmentioning

confidence: 99%

“…A rapid, reagentless on-line analytical technique to sample and detect bioaerosols, at the individual particle-level of resolution, called bioaerosol mass spectrometry (BAMS), is being developed at Lawrence Livermore National Laboratory for national security and public health applications that require detection of airborne bio-agents such as vegetative bacteria, bacterial spores, viruses, and biological toxins (8,34,41). Although the size and cost of BAMS is currently greater than some other techniques, it will be useful where real-time, unmanned, continuous operation is required, and development of a smaller and less-expensive version is under way.…”

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Bioaerosol Mass Spectrometry for Rapid Detection of Individual Airborne Mycobacterium tuberculosis H37Ra Particles

Tobias

Schafer

Pitesky

et al. 2005

Appl Environ Microbiol

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Single-particle laser desorption/ionization time-of-flight mass spectrometry, in the form of bioaerosol mass spectrometry (BAMS), was evaluated as a rapid detector for individual airborne, micron-sized, Mycobacterium tuberculosis H37Ra particles, comprised of a single cell or a small number of clumped cells. The BAMS mass spectral signatures for aerosolized M. tuberculosis H37Ra particles were found to be distinct from M. smegmatis, Bacillus atrophaeus, and B. cereus particles, using a distinct biomarker. This is the first time a potentially unique biomarker was measured in M. tuberculosis H37Ra on a single-cell level. In addition, M. tuberculosis H37Ra and M. smegmatis were aerosolized into a bioaerosol chamber and were sampled and analyzed using BAMS, an aerodynamic particle sizer, a viable Anderson six-stage sampler, and filter cassette samplers that permitted direct counts of cells. In a background-free environment, BAMS was able to sample and detect M. tuberculosis H37Ra at airborne concentrations of >1 M. tuberculosis H37Ra-containing particles/liter of air in 20 min as determined by direct counts of filter cassette-sampled particles, and concentrations of >40 M. tuberculosis H37Ra CFU/liter of air in 1 min as determined by using viable Andersen six-stage samplers. This is a first step toward the development of a rapid, stand-alone airborne M. tuberculosis particle detector for the direct detection of M. tuberculosis bioaerosols generated by an infectious patient. Additional instrumental development is currently under way to make BAMS useful in realistic environmental and respiratory particle backgrounds expected in tuberculosis diagnostic scenarios.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Bioaerosol Mass Spectrometry for Rapid Detection of Individual Airborne Mycobacterium tuberculosis H37Ra Particles

Tobias

Schafer

Pitesky

et al. 2005

Appl Environ Microbiol

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“…Nevertheless, by the analysis of fragment ions that are specific to distinct microorganisms, the types of viruses, bacteria, and fungi present in the air can be assessed on an individual-particle basis. The analysis of biomarker ions forms the basis of bioaerosol mass spectrometry (BAMS), a new and highly efficient method for the detection of both spores and vegetative cells of airborne pathogens and bioterrorism agents (Russell et al, 2004Tobias et al, 2005;van Wuijckhuijse et al, 2005;Beddows and Telle, 2005). AMS is now a widely used single-particle method in aerosol science, with unique capabilities for obtaining species-specific data from biological particles.…”

Section: Physical and Chemical Characterization Of Biological Atmosphmentioning

confidence: 99%

Microbiology and atmospheric processes: biological, physical and chemical characterization of aerosol particles

et al. 2009

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Abstract. The interest in bioaerosols has traditionally been linked to health hazards for humans, animals and plants. However, several components of bioaerosols exhibit physical properties of great significance for cloud processes, such as ice nucleation and cloud condensation. To gain a better understanding of their influence on climate, it is therefore important to determine the composition, concentration, seasonal fluctuation, regional diversity and evolution of bioaerosols. In this paper, we will review briefly the existing techniques for detection, quantification, physical and chemical analysis of biological particles, attempting to bridge physical, chemical and biological methods for analysis of biological particles and integrate them with aerosol sampling techniques. We will also explore some emerging spectroscopy techniques for bulk and single-particle analysis that have potential for in-situ physical and chemical analysis. Lastly, we will outline open questions and further desired capabilities (e.g., in-situ, sensitive, both broad and selective, on-line, time-resolved, rapid, versatile, cost-effective techniques) required prior to comprehensive understanding of chemical and physical characterization of bioaerosols.

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“…In most cases, however, it does not reveal the exact chemical composition of the particle; the desorption and ionization process involves many competing factors 10 that cause certain molecules to be preferentially ionized while the ionization of others can be almost completely suppressed. In spite of this complexity, many of the molecules which constitute the spectral patterns of spores and other agent surrogates analyzed in the BAMS system have now been identified 11,12 .…”

Section: Results From the Reflectron Mass Spectrometermentioning

confidence: 99%

Detection of biological particles in ambient air using bioaerosol mass spectrometry

et al. 2006

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The Bio-Aerosol Mass Spectrometry (BAMS) system is an instrument used for the real time detection and identification of biological aerosols. Particles are drawn from the atmosphere directly into vacuum and tracked as they scatter light from several continuous wave lasers. After tracking, the fluorescence of individual particles is excited by a pulsed 266nm or 355nm laser. Molecules from those particles with appropriate fluorescence properties are subsequently desorbed and ionized using a pulsed 266nm laser. Resulting ions are analyzed in a dual polarity mass spectrometer. During two field deployments at the San Francisco International Airport, millions of ambient particles were analyzed and a small but significant fraction were found to have fluorescent properties similar to Bacillus spores and vegetative cells. Further separation of non-biological background particles from potential biological particles was accomplished using laser desorption/ionization mass spectrometry. This has been shown to enable some level of species differentiation in specific cases, but the creation and observation of higher mass ions is needed to enable a higher level of specificity across more species. A soft ionization technique, matrix-assisted laser desorption/ionization (MALDI) is being investigated for this purpose. MALDI is particularly well suited for mass analysis of biomolecules since it allows for the generation of molecular ions from large mass compounds that would fragment under normal irradiation. Some of the initial results from a modified BAMS system utilizing this technique are described.

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Toward understanding the ionization of biomarkers from micrometer particles by bio-aerosol mass spectrometry

Cited by 33 publications

References 26 publications

Bioaerosol Mass Spectrometry for Rapid Detection of Individual Airborne Mycobacterium tuberculosis H37Ra Particles

Bioaerosol Mass Spectrometry for Rapid Detection of Individual Airborne Mycobacterium tuberculosis H37Ra Particles

Microbiology and atmospheric processes: biological, physical and chemical characterization of aerosol particles

Detection of biological particles in ambient air using bioaerosol mass spectrometry

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