Towards chemical analysis of nanostructures in biofilms I: imaging of biological nanostructures

Schmid, Thomas; Burkhard, Johannes A.; Yeo, Boon Siang; Zhang, Weihua; Zenobi, Renato

doi:10.1007/s00216-008-2100-2

Cited by 43 publications

(39 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Across measurement techniques, reported values of pore size for alginate membranes range between 3.6 nm and 10.9 μm [12,15,16,23,25,37,38,39,40,41,42,43,44,45,46]. For the external gelation fabrication method, the molecular weight cutoff (MWCO) of the membrane has been placed between 60 and 70 kDa (≈3–6 nm) by multiple sources [7,12,20,25,37,47].…”

Section: Resultsmentioning

confidence: 99%

Fickian-Based Empirical Approach for Diffusivity Determination in Hollow Alginate-Based Microfibers Using 2D Fluorescence Microscopy and Comparison with Theoretical Predictions

Mobed-Miremadi

Djomehri

Keralapura³

et al. 2014

Materials

View full text Add to dashboard Cite

Hollow alginate microfibers (od = 1.3 mm, id = 0.9 mm, th = 400 µm, L = 3.5 cm) comprised of 2% (w/v) medium molecular weight alginate cross-linked with 0.9 M CaCl2 were fabricated to model outward diffusion capture by 2D fluorescent microscopy. A two-fold comparison of diffusivity determination based on real-time diffusion of Fluorescein isothiocyanate molecular weight (FITC MW) markers was conducted using a proposed Fickian-based approach in conjunction with a previously established numerical model developed based on spectrophotometric data. Computed empirical/numerical (Dempiricial/Dnumerical) diffusivities characterized by small standard deviations for the 4-, 70- and 500-kDa markers expressed in m2/s are (1.06 × 10−9 ± 1.96 × 10−10)/(2.03 × 10−11), (5.89 × 10−11 ± 2.83 × 10−12)/(4.6 × 10−12) and (4.89 × 10−12 ± 3.94 × 10−13)/(1.27 × 10−12), respectively, with the discrimination between the computation techniques narrowing down as a function of MW. The use of the numerical approach is recommended for fluorescence-based measurements as the standard computational method for effective diffusivity determination until capture rates (minimum 12 fps for the 4-kDa marker) and the use of linear instead of polynomial interpolating functions to model temporal intensity gradients have been proven to minimize the extent of systematic errors associated with the proposed empirical method.

show abstract

Section: Resultsmentioning

confidence: 99%

Fickian-Based Empirical Approach for Diffusivity Determination in Hollow Alginate-Based Microfibers Using 2D Fluorescence Microscopy and Comparison with Theoretical Predictions

Mobed-Miremadi

Djomehri

Keralapura³

et al. 2014

Materials

View full text Add to dashboard Cite

show abstract

“…During bacterial biofilm growth and maturation, cells continue to deposit an extracellular matrix, which plays roles in cell cohesion (106,133,134), gas exchange (104), drug resistance (21,(135)(136)(137), defense against penetration by white blood cells (138)(139)(140)(141)(142), and the maintenance of architecture and biofilm rigidity (106,143,144). The ECM is hydrated and therefore is composed primarily of water.…”

Section: Defining a C Albicans Biofilm: Lessons From Bacteriamentioning

confidence: 99%

Plasticity of Candida albicans Biofilms

Soll

Daniels

2016

Microbiol Mol Biol Rev

View full text Add to dashboard Cite

SUMMARYCandida albicans, the most pervasive fungal pathogen that colonizes humans, forms biofilms that are architecturally complex. They consist of a basal yeast cell polylayer and an upper region of hyphae encapsulated in extracellular matrix. However, biofilms formedin vitrovary as a result of the different conditions employed in models, the methods used to assess biofilm formation, strain differences, and, in a most dramatic fashion, the configuration of the mating type locus (MTL). Therefore, integrating data from different studies can lead to problems of interpretation if such variability is not taken into account. Here we review the conditions and factors that cause biofilm variation, with the goal of engendering awareness that more attention must be paid to the strains employed, the methods used to assess biofilm development, every aspect of the model employed, and the configuration of theMTLlocus. We end by posing a set of questions that may be asked in comparing the results of different studies and developing protocols for new ones. This review should engender the notion that not all biofilms are created equal.

show abstract

“…Hence, many researchers focused on microbial adhesion [5,6]. It has been reported that EPS and filamentous cell appendages facilitate bacterial adhesion between a cell and its surface [7][8][9][10][11]. Therefore, we need to understand the roles of filamentous cell appendages and EPS to reveal the mechanism of biofilm formation.…”

Section: Introductionmentioning

confidence: 99%

Electron microscopy of Staphylococcus epidermidis fibril and biofilm formation using image-enhancing ionic liquid

et al. 2014

View full text Add to dashboard Cite

We established an optimized biofilm observation method using a hydrophilic ionic liquid (IL), 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]). In the present study, a biofilm was formed by Staphylococcus epidermidis. Using field emission (FE) scanning electron microscopy (SEM) and transmission electron microscopy (TEM), the colonization of assemblages formed by microbial cells was observed as a function of the cultivation time. FE-TEM analysis revealed that the fibril comprises three types of protein. In addition, the ultrastructure of each protein monomer was visualized. It was expected that the curly-structured protein plays an important role in extension during fibril formation. Compared to the conventional sample preparation method for electron microscopy, a fine structure was easily obtained by the present method using IL. This observation technique can provide valuable information to characterize the ultrastructure of the fibril and biofilm that has not been revealed till date. Furthermore, these findings of the molecular architecture of the fibril and the colonization behavior of microbial cells during biofilm formation are useful for the development of antibacterial drugs and microbial utilization.

show abstract

Towards chemical analysis of nanostructures in biofilms I: imaging of biological nanostructures

Cited by 43 publications

References 43 publications

Fickian-Based Empirical Approach for Diffusivity Determination in Hollow Alginate-Based Microfibers Using 2D Fluorescence Microscopy and Comparison with Theoretical Predictions

Fickian-Based Empirical Approach for Diffusivity Determination in Hollow Alginate-Based Microfibers Using 2D Fluorescence Microscopy and Comparison with Theoretical Predictions

Plasticity of Candida albicans Biofilms

Electron microscopy of Staphylococcus epidermidis fibril and biofilm formation using image-enhancing ionic liquid

Contact Info

Product

Resources

About