Toxic effect of the novel chiral insecticide IPP and its biodegradation intermediate in nematode Caenorhabditis elegans

Bian, Tingting; Zhu, Xiaolin; Guo, Jiang; Zhuang, Zanyong; Cai, Zhiqiang; Zhao, Xiubo

doi:10.1016/j.ecoenv.2018.08.059

Cited by 14 publications

(6 citation statements)

References 24 publications

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“…The head thrashing frequency and body bent frequency were recorded at 1, 3, 6, and 12 h post-treatment assisted by a dissecting microscope (45×). The head thrashing and body bend frequency were recorded according to a previous method [51]. The MM liquid medium without microorganisms and 6-oxychitin served as a control.…”

Section: Anti-nematode Assay 471 Effect On J2s Locomotion Behaviourmentioning

confidence: 99%

Efficacy of a Chitin-Based Water-Soluble Derivative in Inducing Purpureocillium lilacinum against Nematode Disease (Meloidogyne incognita)

Zhan

Qin

Gao

et al. 2021

IJMS

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Plant-parasitic nematodes cause severe economic losses annually which has been a persistent problem worldwide. As current nematicides are highly toxic, prone to drug resistance, and have poor stability, there is an urgent need to develop safe, efficient, and green strategies. Natural active polysaccharides such as chitin and chitosan with good biocompatibility and biodegradability and inducing plant disease resistance have attracted much attention, but their application is limited due to their poor solubility. Here, we prepared 6-oxychitin with good water solubility by introducing carboxylic acid groups based on retaining the original skeleton of chitin and evaluated its potential for nematode control. The results showed that 6-oxychitin is a better promoter of the nematicidal potential of Purpureocillium lilacinum than other water-soluble chitin derivatives. After treatment, the movement of J2s and egg hatching were obviously inhibited. Further plant experiments found that it can destroy the accumulation and invasion of nematodes, and has a growth-promoting effect. Therefore, 6-oxychitin has great application potential in the nematode control area.

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Section: Anti-nematode Assay 471 Effect On J2s Locomotion Behaviourmentioning

confidence: 99%

Efficacy of a Chitin-Based Water-Soluble Derivative in Inducing Purpureocillium lilacinum against Nematode Disease (Meloidogyne incognita)

Zhan

Qin

Gao

et al. 2021

IJMS

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“…The most complete publications, reported on 26 out of 30 criteria [ 30 ] that we formulated, the most incomplete reported only 15 of 30 criteria [ 31 , 32 ] ( Fig. 1C ).…”

Section: Resultsmentioning

confidence: 99%

Towards a reporting guideline for developmental and reproductive toxicology testing in C. elegans and other nematodes

Voet¹,

Teunis

Haar

et al. 2021

Toxicology Research

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Implementation of reliable methodologies allowing Reduction, Refinement, and Replacement (3Rs) of animal testing is a process that takes several decades and is still not complete. Reliable methods are essential for regulatory hazard assessment of chemicals where differences in test protocol can influence the test outcomes and thus affect the confidence in the predictive value of the organisms used as an alternative for mammals. Although test guidelines are common for mammalian studies, they are scarce for non-vertebrate organisms that would allow for the 3Rs of animal testing. Here, we present a set of 30 reporting criteria as the basis for such a guideline for Developmental and Reproductive Toxicology (DART) testing in the nematode Caenorhabditis elegans. Small organisms like C. elegans are upcoming in new approach methodologies for hazard assessment; thus, reliable and robust test protocols are urgently needed. A literature assessment of the fulfilment of the reporting criteria demonstrates that although studies describe methodological details, essential information such as compound purity and lot/batch number or type of container is often not reported. The formulated set of reporting criteria for C. elegans testing can be used by (i) researchers to describe essential experimental details (ii) data scientists that aggregate information to assess data quality and include data in aggregated databases (iii) regulators to assess study data for inclusion in regulatory hazard assessment of chemicals.

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“…43,44 Furthermore, pesticides including paichongding and spirotramat interrupted life cycle and shortened lifespan of nematodes. 45,46 These findings implied that exposure to environmental xenobiotics such as fluopimomide adversely affected survival of C. elegans. An increase in ROS accumulation indicated that the nematodes were under oxidative stress, which was considered to be an important factor leading to aging.…”

Section: Exposure To Fluopimomide At Sublethal Doses Shortened Lifesp...mentioning

confidence: 97%

Exposure to fluopimomide at sublethal doses causes oxidative stress in Caenorhabditis elegans regulated by insulin/insulin‐like growth factor 1‐like signaling pathway

Zhang

Liu

et al. 2022

Environmental Toxicology

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Fluopimomide is an innovative pesticide, widely used for agricultural pest management; however, little is known about its effect on non‐target organisms. This study was designed to assess the potential risk of fluopimomide and the molecular mechanisms using Caenorhabditis elegans, a common model animal. The oxidative stress‐related indicators were analyzed in C. elegans after exposure to fluopimomide for 24 h at three sublethal doses (0.2, 1.0, and 5.0 mg/L). The results demonstrated that sublethal exposure to fluopimomide adversely affected the nematodes growth, locomotive behaviors, reproduction, and lifespan, accompanying with enhanced of reactive oxygen species (ROS) generation, lipid and lipofuscin accumulation, and malondialdehyde content. In addition, exposure to fluopimomide significantly inhibited antioxidant systems including superoxide dismutase, catalase, glutathione S‐transferase, and glutathione in the nematodes. Moreover, the expression of oxidative stress‐related genes of sod‐3, hsp‐16.1, gst‐4, ctl‐2, daf‐16, and daf‐2 were significantly down‐regulated, while the expression of skn‐1 was significantly up‐regulated. Further evidence revealed that daf‐16 and skn‐1 mutant strains of C. elegans significantly decreased ROS production upon fluopimomide exposure compared with the wild‐type nematodes. Overall, our findings indicated that exposure to fluopimomide at sublethal doses caused oxidative damage, mainly associated with insulin/IGF‐1‐like signaling pathway in C. elegans. This is the first report of potential toxic effects of fluopimomide even at low concentrations, providing a new insight into the mechanisms of toxicity to C. elegans by fluopimomide.

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Toxic effect of the novel chiral insecticide IPP and its biodegradation intermediate in nematode Caenorhabditis elegans

Cited by 14 publications

References 24 publications

Efficacy of a Chitin-Based Water-Soluble Derivative in Inducing Purpureocillium lilacinum against Nematode Disease (Meloidogyne incognita)

Efficacy of a Chitin-Based Water-Soluble Derivative in Inducing Purpureocillium lilacinum against Nematode Disease (Meloidogyne incognita)

Towards a reporting guideline for developmental and reproductive toxicology testing in C. elegans and other nematodes

Exposure to fluopimomide at sublethal doses causes oxidative stress in Caenorhabditis elegans regulated by insulin/insulin‐like growth factor 1‐like signaling pathway

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