Toxic effects of quinacrine hydrochloride in rhesus monkeys

Chandra, Harish; Malaviya, B

doi:10.1016/0010-7824(81)90039-1

Cited by 8 publications

(1 citation statement)

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“…The most studied chemical agent used to create tubal sclerosis was quinacrine hydrochloride [5], [6]. Development of this approach was stopped due to concerns over the potential toxicity of quinacrine [7]. A safer alternative sclerosing agent is polidocanol (hydroxy-polyethoxy-dodecane), a synthetic long-chain fatty alcohol that has a high therapeutic index of safety [8].…”

Section: Introductionmentioning

confidence: 99%

Polidocanol induced tubal occlusion in nonhuman primates: immunohistochemical detection of collagen I-V

et al. 2016

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ObjectiveIntrauterine administration of polidocanol foam (PF) can create fallopian tube occlusion in nonhuman primates. The objective of this study was to determine if PF-induced tubal obstructions contain collagen in the extracellular matrix.Study designWe compared tissue samples of the intramural fallopian tube obtained from previous studies evaluating the effects of intrauterine infusion of 5% PF 2–12 weeks after treatment. Serial sections of the intramural portion of the fallopian tube obtained from representative treated (rhesus macaques, n=7; baboon, n=11) and untreated control (macaque, n=3; baboon, n=5) animals were stained with hematoxylin and eosin to identify tubal occlusion and by immunohistochemistry for collagens Col-I, Col-III and Col-IV. Descriptive results are summarized.ResultsControl animals exhibited histologically normal fallopian tubal epithelium with no staining for Col-1, light staining for Col-III and Col-V in the lamina propria and Col-IV distributed evenly in the extracellular matrix of the lamina propria. Treatment with PF resulted in acute tissue damage confined to the intramural tube; no epithelial damage or occlusion occurred in the tubal isthmus or ampulla. Blockade of the intramural tube demonstrated fibrosis with the epithelium replaced with extracellular matrix that stained strongly for Col-I, Col-III, Col-IV and Col-V. Col-II was undetectable.ConclusionTubal blockage induced by PF resulted in loss of normal epithelium and accumulation of collagens Col-I, Col-III, Col-IV and Col-V at the site of obstruction. The presence of dense collagen staining supports the hypothesis that PF infusion creates lasting tubal obstructions.ImplicationsThis study demonstrates that PF-induced tubal occlusion results in deposition of collagens suggesting the potential for a more lasting blockade. The structural nature of this occlusion supports the development of intrauterine administration of PF as a nonsurgical method of permanent contraception.

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Section: Introductionmentioning

confidence: 99%