Toxic mechanisms of copper oxide nanoparticles in epithelial kidney cells

Thit, Amalie; Selck, Henriette; Bjerregaard, Henning F.

doi:10.1016/j.tiv.2015.03.020

Cited by 75 publications

(55 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, the cell death was minimal when the same concentration of CuCl 2 salt was exposed to the cellular system studied compared to the CuO NPs [Figure 8(B) and (D)] reasonably agreeing with the literature. 25, 73 …”

Section: Resultsmentioning

confidence: 99%

Safe-by-Design CuO NanoparticlesviaFe-Doping, Cu–O Bond Length Variation, and Biological Assessment in Cells and Zebrafish Embryos

Naatz¹,

et al. 2017

View full text Add to dashboard Cite

The safe implementation of nanotechnology requires nanomaterial hazard assessment in accordance with the material physicochemical properties that trigger the injury response at the nano/bio interface. Since CuO nanoparticles (NPs) are widely used industrially and their dissolution properties play a major role in hazard potential, we hypothesized that tighter bonding of Cu to Fe by particle doping could constitute a safer-by-design approach through decreased dissolution. Accordingly, we designed a combinatorial library in which CuO was doped with 1–10% Fe in a flame spray pyrolysis (FSP) reactor. The morphology and structural properties were determined by XRD, BET, Raman spectroscopy, HRTEM, EFTEM and EELS, which demonstrated a significant reduction in the apical Cu-O bond length while simultaneously increasing the planar bond length (Jahn-Teller distortion). Hazard screening was performed in tissue culture cell lines and zebrafish embryos to discern the change in the hazardous effects of doped vs. non-doped particles. This demonstrated that with increased levels of doping, there was a progressive decrease in cytotoxicity in BEAS-2B and THP-1 cells, as well as an incremental decrease in the rate of hatching interference in zebrafish embryos. The dissolution profiles were determined and the surface reactions taking place in Holtfreter’s solution were validated using cyclic voltammetry (CV) measurements to demonstrate the Cu+/Cu2+ and Fe2+/Fe3+ redox species playing a major role in the dissolution process of pure and Fe doped CuO. Altogether, a safe-by-design strategy was implemented for the toxic CuO particles via Fe doping and has been demonstrated for their safe use in the environment.

show abstract

Section: Resultsmentioning

confidence: 99%

Safe-by-Design CuO NanoparticlesviaFe-Doping, Cu–O Bond Length Variation, and Biological Assessment in Cells and Zebrafish Embryos

Naatz¹,

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Even though the molecular toxicity mechanisms of CUNPs on eukaryotic cells are unclear, many studies have shown that these nanoparticles promote mitochondrial damage, DNA damage, and oxidative DNA damage . These results showed that CUNPs were more toxic than Cu 2+ because of the generation of ROS, DNA damage, and decreased levels of reduced glutathione (GSH) compared with those of control cells.…”

Section: Resultsmentioning

confidence: 99%

Biogenic synthesis of copper oxide nanoparticles using olea europaea leaf extract and evaluation of their toxicity activities: An in vivo and in vitro study

Sulaiman

Tawfeeq

Jaaffer

2017

Biotechnology Progress

114

View full text Add to dashboard Cite

Copper oxide nanoparticles (CUNPs) were synthesized using Olea europaea leaf extract as reducing and protecting agent. The formation of nanoparticles was observed through a color change from yellowish to brownish black. The CUNPs were confirmed with UV-Vis spectrophotometer, which revealed a peak absorbance at 289 nm. The synthesized CUNPs were characterized by XRD, FTIR, SEM, and TEM. The XRD pattern revealed that CUNPs were crystalline in nature with a diameter around 20 nm. FTIR spectral analysis showed that CUNPs were capped with plant constituents. From SEM and TEM analyses, the CUNPs were generally found to be spherical in shape, and the size range was 20-50 nm. Free radical scavenging potential of CUNPs against DPPH was confirmed by its stable antioxidant effects. In addition, the toxicity of CUNPs in mice was also assessed by body weight and weights of liver, kidneys, spleen, and thymus. The immune response in mice was signaled through an obvious change in spleen and thymus index, with a decrease of ADA enzyme activity in serum, spleen, and thymus after CUNPs treatment. The CUNPs were found to exert cell growth arrest against AMJ-13 and SKOV-3 cancer cells in a dose-dependent manner and induce cell death by apoptosis. Less significant cytotoxic effect was observed in normal dermal fibroblast cells. These findings suggest that CUNPs may have the potential to be anticancer agents. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:218-230, 2018.

show abstract

“…Intercellular generated reactive oxygen species (ROS, H 2 O 2 ,

{normalO}_{2}^{\cdot -}

, and OH · ) adversely oxide proteins, lipids, and DNA. Subsequent DNA damage may have activated signaling pathway that induces cell‐cycle arrest, eventually leading to cell death, normally via apoptosis …”

Section: Discussionmentioning

confidence: 99%

Matrix metalloproteases inhibition and biocompatibility of gold and platinum nanoparticles

Hashimoto

Kawai

Kawakami

et al. 2015

J Biomedical Materials Res

View full text Add to dashboard Cite

Matrix metalloprotease (MMP) inhibitors improve the longevity of dental adhesives/tooth bonds; however, biocompatibility is required for their clinical use. This study evaluated the inhibition of MMPs and toxicity of two gold (AuNPs) and platinum nanoparticles (PtNPs) as possible compounds for use in dental adhesives. The MMP assay for studying the interaction of MMPs and nanoparticles (NPs) was evaluated by an MMP assay kit and gelatin zymography. Cultured L929 fibroblast cells or RAW264 macrophages were exposed to NPs. The cellular responses to NPs were examined using cytotoxic (cell viability) and genotoxic assays (comet assay), and transmission electron microscopic (TEM) analysis. The mechanical properties (elastic modulus) of the experimental resin loaded with NPs were examined using thermomechanical analysis. All NPs inhibited MMP activity at relatively low concentrations. The NPs inhibit MMPs by chelating with the Zn(2+) bound in the active sites of MMPs. No cytotoxic and genotoxic effects were found in AuNPs, whereas the PtNPs possessed both adverse effects. In TEM analysis, the NPs were localized mainly in lysosomes without penetration into nuclei. The mechanical properties of the resins increased when AuNPs were added in resins, but not by PtNPs. AuNPs are attractive candidates to inhibit MMPs and improve the mechanical properties of resins without cytotoxic/genotoxic effects to cells, and therefore should be suitable for applications in adhesive resin systems.

show abstract

Toxic mechanisms of copper oxide nanoparticles in epithelial kidney cells

Cited by 75 publications

References 41 publications

Safe-by-Design CuO NanoparticlesviaFe-Doping, Cu–O Bond Length Variation, and Biological Assessment in Cells and Zebrafish Embryos

Safe-by-Design CuO NanoparticlesviaFe-Doping, Cu–O Bond Length Variation, and Biological Assessment in Cells and Zebrafish Embryos

Biogenic synthesis of copper oxide nanoparticles using olea europaea leaf extract and evaluation of their toxicity activities: An in vivo and in vitro study

Matrix metalloproteases inhibition and biocompatibility of gold and platinum nanoparticles

Contact Info

Product

Resources

About