Toxic trace metals and embryo quality indicators during in vitro fertilization (IVF)

Bloom, Michael S.; Parsons, Patrick J.; Kim, Dongsul; Steuerwald, Amy J.; Vaccari, Sergio; Cheng, Gloria; Fujimoto, Victor Y.

doi:10.1016/j.reprotox.2010.11.011

Cited by 32 publications

(24 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We previously reported an inverse association between blood Pb concentrations in IVF patients and embryo cleavage rate measured as ECN (OR00.25, 95%CI 0.07-0.86), adjusted for age, cigarette smoking, race/ethnicity, blood Hg and urine Cd concentrations; we detected no association for EFS [18]. We do not detect an association in this study for Hg, Cd or Pb with ECN or with EFS.…”

Section: Associations Between Metals and Embryo Qualitycontrasting

confidence: 89%

“…Collected embryos were grouped into tertiles of ECN (1-5 cells/6-7 cells/8-12 cells) and EFS (1/2/3-5) [18]. Under the assumption of proportional odds, ordinal logistic regression estimates the log-odds of a subject's outcome falling into a higher ordered endpoint category [37].…”

Section: Discussionmentioning

confidence: 99%

“…Sample selection and clinical protocols were previously described in detail [17,18]. Briefly, female patients underwent gonadotropininduced ovarian stimulation per clinic protocols.…”

Section: Sample Selection and Clinical Protocolmentioning

confidence: 99%

“…Approximately 16-18 h following insemination, zygotes were identified by the appearance of two pronuclei. A single embryologist who was blinded to exposure data examined all embryos produced by a couple as previously described in detail [18]. Embryo cell number (ECN) was assessed on the day of transfer and characterizes cleavage or growth rate; it is a positive predictor for IVF success.…”

Section: Sample Selection and Clinical Protocolmentioning

confidence: 99%

“…Studies report decreased fecundity or fertility [7][8][9][10][11][12], no association [13,14], or even increased fecundity [7,15] in association with higher exposures. In our studies, we conducted preliminary evaluations of trace metal concentrations measured in blood and urine specimens collected from women undergoing in vitro fertilization (IVF), and reported associations between blood Pb and oocyte maturity [16,17], urine Cd and oocyte fertilization [17], blood Pb level and embryo cleavage [18], and blood Hg and Cd and embryo implantation [19].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Associations between toxic metals in follicular fluid and in vitro fertilization (IVF) outcomes

Bloom

Kim

Kruger

et al. 2012

J Assist Reprod Genet

Self Cite

View full text Add to dashboard Cite

Purpose We previously reported associations between trace concentrations of Hg, Cd and Pb in blood and urine and reproductive outcomes for women undergoing in-vitro fertilization (IVF). Here we assess measurements in single follicular fluid (FF) specimens from 46 women as a presumably more relevant marker of dose for reproductive toxicity. Methods FF specimens were analyzed for Hg, Cd and Pb using sector field-inductively coupled plasma-mass spectrometry (SF-ICP-MS). Variability sources were assessed by nested ANOVA. Multivariable regression was used to evaluate associations for square root transformed metals with IVF outcomes, adjusting for confounders.

show abstract

Section: Associations Between Metals and Embryo Qualitycontrasting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

“…Sample selection and clinical protocols were previously described in detail [17,18]. Briefly, female patients underwent gonadotropininduced ovarian stimulation per clinic protocols.…”

Section: Sample Selection and Clinical Protocolmentioning

confidence: 99%

Section: Sample Selection and Clinical Protocolmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Associations between toxic metals in follicular fluid and in vitro fertilization (IVF) outcomes

Bloom

Kim

Kruger

et al. 2012

J Assist Reprod Genet

Self Cite

View full text Add to dashboard Cite

show abstract

Role of Environmental Factors and Gonadotoxin Exposure in Unexplained Female Infertility

Fujimoto

Bloom

2015

Unexplained Infertility

Self Cite

View full text Add to dashboard Cite

Urine cortisol concentration as a biomarker of stress is unrelated to IVF outcomes in women and men

Butts

Bloom

Frye

et al. 2014

J Assist Reprod Genet

Self Cite

View full text Add to dashboard Cite

Purpose Our primary objective was to assess associations between urine cortisol as a biomarker of psychological stress and in vitro fertilization (IVF) outcomes. A secondary objective was to assess associations between toxic metals and cortisol. Methods Urine and blood specimens were collected from 52 women and 28 male partners completing a first IVF procedure, on the day of oocyte retrieval. Urine cortisol was measured with an enzyme-linked immunosorbent assay. Mercury (Hg), cadmium (Cd), and lead (Pb) were determined in blood and Cd in urine by inductively coupled plasma-mass spectrometry.Results No associations were indicated for cortisol with IVF outcomes in multivariable regression models adjusted for covariates. However, we detected positive linear associations for cortisol and urine Cd (β=9.96, 95%CI 1.52, 21.44) and blood Hg (β=1.44, 95%CI 0.31, 3.18). An exploratory stratified analysis suggested a potential inverse association between urine cortisol and oocyte fertilization among women with low, but not high blood Hg. Conclusion While limited, these preliminary data suggest that psychological stress may not play a major role in IVF outcomes, which therefore could be one less concern for couples and their clinicians. Our data also raise the possibility for toxic metals to modify associations between cortisol and IVF outcomes among women. However, these preliminary results require corroboration in an experimental animal model and confirmation in a larger, more definitive observational study.

show abstract

Toxic trace metals and embryo quality indicators during in vitro fertilization (IVF)

Cited by 32 publications

References 46 publications

Associations between toxic metals in follicular fluid and in vitro fertilization (IVF) outcomes

Associations between toxic metals in follicular fluid and in vitro fertilization (IVF) outcomes

Role of Environmental Factors and Gonadotoxin Exposure in Unexplained Female Infertility

Urine cortisol concentration as a biomarker of stress is unrelated to IVF outcomes in women and men

Contact Info

Product

Resources

About