Toxicity and anthelmintic efficacy of chitosan encapsulated bromelain against gastrointestinal strongyles in Small East African goats in Kenya

Wasso, Shukuru; Maina, Naomi; Kagira, John

doi:10.14202/vetworld.2020.177-183

Cited by 10 publications

(17 citation statements)

References 33 publications

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“…Bromelain by enzymatic digestion of structural proteins present in the nematode's cuticle leads to damage and the final loss of motility by parasites. A novel approach to the use of bromelain in the treatment of parasitic nematodes was proposed by Wasso et al [92], who evaluated in vitro and in vivo the anthelmintic efficacy of chitosan encapsulated bromelain against Haemonchus contortus. Domingues et al [93] and Luoga et al [94].…”

Section: Anthelmintic Efficacymentioning

confidence: 99%

Beneficial Properties of Bromelain

Hikisz

Bernasińska-Słomczewska

2021

Nutrients

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Bromelain is a major sulfhydryl proteolytic enzyme found in pineapple plants, having multiple activities in many areas of medicine. Due to its low toxicity, high efficiency, high availability, and relative simplicity of acquisition, it is the object of inexhaustible interest of scientists. This review summarizes scientific reports concerning the possible application of bromelain in treating cardiovascular diseases, blood coagulation and fibrinolysis disorders, infectious diseases, inflammation-associated diseases, and many types of cancer. However, for the proper application of such multi-action activities of bromelain, further exploration of the mechanism of its action is needed. It is supposed that the anti-viral, anti-inflammatory, cardioprotective and anti-coagulatory activity of bromelain may become a complementary therapy for COVID-19 and post-COVID-19 patients. During the irrepressible spread of novel variants of the SARS-CoV-2 virus, such beneficial properties of this biomolecule might help prevent escalation and the progression of the COVID-19 disease.

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Section: Anthelmintic Efficacymentioning

confidence: 99%

Beneficial Properties of Bromelain

Hikisz

Bernasińska-Słomczewska

2021

Nutrients

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“…The study was conducted for a period of 5 months (February 2021 to June 2021). The study was carried out at Jomo Kenyatta University of Agriculture Technology, Juja, Kiambu County, Kenya located at latitude 1°05 S and longitude 37°00 E. It lies at an altitude of 1525 m above sea level and receives an annual rainfall of 850 mm with an average temperature of 18.7°C [16].…”

Section: Study Period and Locationmentioning

confidence: 99%

“…One plant that has been shown to exert appreciable levels of antiparasitic activity is the pineapple (Ananas comosus). The pineapple is a common tropical fruit grown in various countries, including Kenya [16]. Bromelain is the main compound extracted from pineapples and has been characterized as a mixture of cysteine proteases found in the tissues of plants from the Bromeliaceae family.…”

Section: Introductionmentioning

confidence: 99%

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In vitro anticoccidial activity of nanoencapsulated bromelain against Eimeria spp. oocysts isolated from goats in Kenya

Daiba¹,

Kagira

Ngotho

et al. 2022

Vet World

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Background and Aim: The emergence of drug-resistant strains of Eimeria spp. calls for the development of novel anticoccidial drugs. Plant extracts provide a possible natural source for such drugs. This study aimed to investigate the in vitro anticoccidial activity of encapsulated bromelain (EB) in chitosan nanocarriers on Eimeria spp. oocysts isolated from goats kept by farmers in Kenya. Materials and Methods: Bromelain was extracted from the peel of ripe pineapples using standard methods. Eimeria spp. oocysts were isolated from the feces of goats using a flotation method. The inhibition of sporulation was assayed after exposing the oocysts to solutions of EB, non-EB (NEB), and diclazuril (positive control) at concentrations between 4 mg/mL and 0.125 mg/mL for 48 h. The oocysts were examined under a microscope (40x) to determine the effects of the drugs on the sporulation process. The percentage of sporulation inhibition was calculated after 48 h and the inhibition concentration 50% (IC50) was determined by probit analysis. Results: Bromelain manifested anticoccidial activity through the inhibition of the sporulation of coccidia oocysts. EB achieved inhibition with a lower dose compared with NEB. The IC50 values of diclazuril, EB, and NEB were 0.078 mg/mL, 0.225 mg/mL, and 0.575 mg/mL, respectively. There were significant differences (p<0.01) between the IC50 of EB and NEB compared with the standard treatment drug. Conclusion: This preliminary study showed that EB has anticoccidial activity supporting further evaluation at an in vivo level to develop a novel drug for the management of coccidiosis in goats.

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“…Encapsulated bromelain was non-toxic as no pathological and histological changes were observed at concentrations ranging from 3 to 30 mg/kg after necropsy. The hematological parameters also remained unchanged at the same concentrations, confirming the non-toxic effect of bromelain in vivo ( 32 ). Encapsulated E. staigeriana essential oil (EnEsEO) was found non-toxic when orally administered to gerbils at 500 mg/kg concentration ( 17 ).…”

Section: Discussionmentioning

confidence: 52%

Nanoparticles as Alternatives for the Control of Haemonchus contortus: A Systematic Approach to Unveil New Anti-haemonchiasis Agents

et al. 2021

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Haemonchus contortus is an infectious gastrointestinal nematode parasite of small ruminants. This study addresses the in vitro/in vivo anti-haemonchiasis potential, toxicological effects, and mechanism of action of nanoparticles. Online databases were used to search and retrieve the published literature (2000 to 2021). A total of 18 articles were selected and reviewed, out of which, 13 (72.2%) studies reported in vitro, 9 (50.0%) in vivo, and 4 (22.2%) both in vitro/in vivo efficacy of different nanoparticles. Mostly, organic nanoparticles (77.7%) were used including polymeric (85.7%) and lipid nanoparticles (14.3%). The highest efficacy, in vitro, of 100% resulted from using encapsulated bromelain against eggs, larvae, and adult worm mortality at 4, 2, and 1 mg/ml, respectively. While in vivo, encapsulated Eucalyptus staigeriana oil reduced worm burden by 83.75% and encapsulated Cymbopogon citratus nano-emulsion by 83.1%. Encapsulated bromelain, encapsulated Eucalyptus staigeriana oil, and encapsulated Cymbopogon citratus nano-emulsion were safe and non-toxic in vivo. Encapsulated bromelain damaged the cuticle, caused paralysis, and death. Nanoparticles could be a potential source for developing novel anthelmintic drugs to overcome the emerging issue of anthelmintic resistance in H. contortus. Studies on molecular effects, toxicological consequences, and different pharmacological targets of nanoparticles are required in future research.

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Toxicity and anthelmintic efficacy of chitosan encapsulated bromelain against gastrointestinal strongyles in Small East African goats in Kenya

Cited by 10 publications

References 33 publications

Beneficial Properties of Bromelain

Beneficial Properties of Bromelain

In vitro anticoccidial activity of nanoencapsulated bromelain against Eimeria spp. oocysts isolated from goats in Kenya

Nanoparticles as Alternatives for the Control of Haemonchus contortus: A Systematic Approach to Unveil New Anti-haemonchiasis Agents

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