Toxicity of CuO nanoparticles and Cu ions to tight epithelial cells from Xenopus laevis (A6): Effects on proliferation, cell cycle progression and cell death

Thit, Amalie; Selck, Henriette; Bjerregaard, Henning F.

doi:10.1016/j.tiv.2012.12.013

Cited by 51 publications

(24 citation statements)

References 37 publications

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“…The three forms of copper tested, (Cu2+; CuO nanoparticles (6 nm) and CuO nanoparticles polydisperse (poly-CuO) caused an increase of cell death and altered cell cycle progression, being polyCuO the one that caused the most serious effects. It may be considered that the poly-CuO particles were highly polydisperse, with sizes ranging from 40 to 500 nm in deionized water, with a majority measuring around 100 nm, and therefore containing particles, both within and outside the defined nano scale (Thit et al, 2013). To test the consequences of the exposure of brain cells to CuO nanoparticles, with a diameter of about 5 nm, cultures of primary brain astrocytes were exposed to CuO nanoparticles, where increased levels of intracellular copper were observed, affecting the cell viability which depends on concentration and temperature.…”

Section: Discussionmentioning

confidence: 99%

Copper Nanoparticles as Potential Antimicrobial Agent in Disinfecting Root Canals: A Systematic Review

Sánchez‐Sanhueza

Fuentes‐Rodríguez

Bello-Toledo

2016

Int. J. Odontostomat.

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ABSTRACT:Copper was registered as the first solid antimicrobial material. Its availability makes it an important option as an antibacterial agent. At nanoparticle size it does not exceed 100 nm, allowing close interaction with microbial membranes, enhancing its effect even more. Copper generates toxic hydroxyl radicals that damage cell membranes of Gram-negative and Gram-positive bacteria, among the latter, Enterococcus faecalis, which are present in infected radicular canals. Synthesis of metal nanoparticles with antimicrobial properties has become a viable alternative and has promising applications in the fight against pathogenic microorganisms. Furthermore, the use of some polymers to stabilize nanoparticles increases their release time and may as well decrease the risk of bacterial recolonization and biofilm formation within the ducts, enhancing the antimicrobial properties of these compounds.The aim of this article is to conduct a systematic review of the literature on antimicrobial copper nanoparticles, their current applications and their potential use in the area of oral health, specifically in the field of endodontics.

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Section: Discussionmentioning

confidence: 99%

Copper Nanoparticles as Potential Antimicrobial Agent in Disinfecting Root Canals: A Systematic Review

Sánchez‐Sanhueza

Fuentes‐Rodríguez

Bello-Toledo

2016

Int. J. Odontostomat.

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“…Development in large-scale production for both metallic and nonmetallic nanoparticles has introduced risk to the environment and human health [18,19]. Improper disposal of nanomaterial waste by labs as well as industry is an alarming threat to the ecosystem as well as aquatic life.…”

Section: Introductionmentioning

confidence: 99%

Plant Mediated Green Synthesis of CuO Nanoparticles: Comparison of Toxicity of Engineered and Plant Mediated CuO Nanoparticles towards Daphnia magna

et al. 2016

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Research on green production methods for metal oxide nanoparticles (NPs) is growing, with the objective to overcome the potential hazards of these chemicals for a safer environment. In this study, facile, ecofriendly synthesis of copper oxide (CuO) nanoparticles was successfully achieved using aqueous extract of Pterospermum acerifolium leaves. P. acerifolium-fabricated CuO nanoparticles were further characterized by UV-Visible spectroscopy, field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray (EDX), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and dynamic light scattering (DLS). Plant-mediated CuO nanoparticles were found to be oval shaped and well dispersed in suspension. XPS confirmed the elemental composition of P. acerifolium-mediated copper nanoparticles as comprised purely of copper and oxygen. DLS measurements and ion release profile showed that P. acerifolium-mediated copper nanoparticles were more stable than the engineered CuO NPs. Copper oxide nanoparticles are used in many applications; therefore, their potential toxicity cannot be ignored. A comparative study was performed to investigate the bio-toxic impacts of plant-synthesized and engineered CuO nanoparticles on water flea Daphnia. Experiments were conducted to investigate the 48-h acute toxicity of engineered CuO NPs and plant-synthesized nanoparticles. Lower EC50 value 0.102 ± 0.019 mg/L was observed for engineered CuO NPs, while 0.69 ± 0.226 mg/L was observed for plant-synthesized CuO NPs. Additionally, ion release from CuO nanoparticles and 48-h accumulation of these nano CuOs in daphnids were also calculated. Our findings thus suggest that the contribution of released ions from nanoparticles and particles/ions accumulation in Daphnia needs to be interpreted with care.

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“…However, this classification of dissolved Cu lumps together dissolved ions, nanoparticles (generally defined as particles with at least one dimension between 1 and 100 nm) and some bulk particles (100e450 nm in size). For proper risk assessment, it is necessary to determine how much of these different fractions of Cu are leached from antifouling paints since their fate, bioavailability, and toxicity differ considerably (Bielmyer-Fraser et al, 2014;Shi et al, 2011;Siddiqui et al, 2015;Thit et al, 2013;Torres-Duarte et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Release and detection of nanosized copper from a commercial antifouling paint

et al. 2016

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a b s t r a c tOne major concern with the use of antifouling paints is the release of its biocides (mainly copper and zinc) into natural waters, where they may exhibit toxicity to non-target organisms. While many studies have quantified the release of biocides from antifouling paints, very little is known about the physicochemical state of released copper. For proper risk assessment of antifouling paints, characterization of copper released into water is necessary because the physicochemical state determines the metal's environmental fate and effects. In this study, we monitored release of different fractions of copper (dissolved, nano, and bulk) from a commercial copper-based antifouling paint. Release from painted wood and aluminum mini-bars that were submerged in natural waters was monitored for 180 days. Leachates contained both dissolved and particulate copper species. X-ray diffraction and X-ray photoelectron spectroscopy were used to determine the chemical phase of particles in the leachate. The amount of copper released was strongly dependent on water salinity, painted surface, and paint drying time. The presence of nanosized Cu 2 O particles was confirmed in paint and its leachate using singleparticle inductively coupled plasma-mass spectrometry and electron microscopy. Toxicity of paint leachate to a marine phytoplankton was also evaluated.

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Toxicity of CuO nanoparticles and Cu ions to tight epithelial cells from Xenopus laevis (A6): Effects on proliferation, cell cycle progression and cell death

Cited by 51 publications

References 37 publications

Copper Nanoparticles as Potential Antimicrobial Agent in Disinfecting Root Canals: A Systematic Review

Copper Nanoparticles as Potential Antimicrobial Agent in Disinfecting Root Canals: A Systematic Review

Plant Mediated Green Synthesis of CuO Nanoparticles: Comparison of Toxicity of Engineered and Plant Mediated CuO Nanoparticles towards Daphnia magna

Release and detection of nanosized copper from a commercial antifouling paint

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