TP53 Codon 72 Polymorphism and Breast Cancer in Northern Iran

Abstract

The p53 gene is one of the most extensively studied human genes because of its role as a tumor suppressor gene. Its diverse functions include DNA binding, cell cycle control, DNA repair, differentiation, genomic plasticity, and apoptosis. A common polymorphism of the p53 gene at codon 72 has been associated with human cancer susceptibility and prognosis. In this study, we investigated p53 codon 72 polymorphism in 42 breast cancer cases and 60 healthy individual in northern Iran. Genomic DNA was extracted from fresh tumor tissues of patients and blood samples of healthy individuals. AS-PCR method was applied for determination of codon 72 polymorphism. The distribution of genotypes in breast cancer cases and controls were different (p = 0.001). Pro allele was significantly associated with the presence of breast cancer (odds ratio = 1.5 and 95% confidence interval = 0.85-2.63). Pro/Pro genotype was overrepresented in breast cancer. Based on these data, it is suggested that Pro allele may modify the risk of breast cancer in northern Iranian women.

The 72Pro Variant of the Tumor Protein 53 Is Associated with an Increased Breast Cancer Risk in the Moroccan Population

“…These results are in agreement with several previous studies, which have reported an increased BC risk in association with Pro/Pro homozygous genotype [21][22][23][24][25][26][27][28]. Similarly, Tp53 Pro allele has been found to be associated with BC risk in North Indian [23], Austrian [22], Bangladeshi [28], Iranian [21], Turkish [30], Spanish [32], Swedish [26], American [34], German [33], Russian [36], Japanese [25], and Slovakian [35] populations.…”

Lack of Association of Intron 3 16 bp Polymorphism of TP53 with Breast Cancer among Iranian-Azeri Patients

“…This may interpret those inconsistent findings. In Iran, we have several reports that tried to assess the association between the TP53 codon 72 polymorphism and different cancer (Kazemi et al, 2009;Salehi and Hadavi 2009;Doosti et al, 2011). To our knowledge, there is only one report regarding PIN3and breast cancer among Iranian patients.…”

p.R72P, PIN3 Ins16bp Polymorphisms of TP53 and CCR5Δ32 in North Indian Breast Cancer Patients

“…An allele specific PCR assay was used to detect either the arginine (Arg) or the proline (Pro) allele using published primer sequences (Kazemi et al, 2009). A negative control without DNA template was included in each reaction.…”