Trace analysis of tobramycin in human plasma by derivatization and high-performance liquid chromatography with ultraviolet detection

Feng, Chia‐Hsien; Lin, Shuo; Wu, Hsin‐Lung; Chen, Su‐Hwei

doi:10.1016/s1570-0232(02)00544-5

Cited by 56 publications

(39 citation statements)

References 26 publications

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“…The bactericidal activity of tobramycin (O-3-amino-3-deoxy-a-D-glucopyranosyl-(1-6)-O-[2,6 diamino-2,3,6-trideoxy-a-D-ribohexopyranosyl-(1Y4)]-2-deoxy-D-streptamine) is accomplished by irreversibly binding to 30S and 50S ribosomal subunits resulting in a defective protein (9).…”

Section: Introductionmentioning

confidence: 99%

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Formulation and Characterization of Lipid-Coated Tobramycin Particles for Dry Powder Inhalation

2006

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Lipid coating of tobramycin formulations resulted in a reduced agglomeration tendency and in high fine particle fraction values, thus improving drug deposition. The very low excipients content (about 5% m/m) of these formulations offers the benefit of delivering particularly huge concentrations of antibiotic directly to the site of infection, while minimizing systemic exposure, and may provide a valuable alternative treatment of cystic fibrosis.

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Section: Introductionmentioning

confidence: 99%

“…Like other aminoglycosides, tobramycin has a comparably narrow safety margin. The therapeutic plasma concentration of tobramycin is in the range of 4Y8 mg/L and may cause severe ototoxicity and nephrotoxicity in a long-term therapy (9).…”

Section: Introductionmentioning

confidence: 99%

Formulation and Characterization of Lipid-Coated Tobramycin Particles for Dry Powder Inhalation

2006

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“…Close monitoring of the contents of aminoglycosides in patients' sera is essential as elevated levels of aminoglycosides antibiotic can cause adverse or toxic reactions such as nephrotoxicity and ototoxicity. Traditionally, microbiological assays (enzymatic assay and immunoassay) [37][38][39] and chromatographic assays (GC and LC) [40][41][42] were employed for clinical analysis. However, long incubation time, interference caused by nonspecific inhibitors, and poor repeatability and reproducibility prevents the former from becoming the method of choice while the latter is not ideal as well due to the needs for pre/on-column derivatization and incompatible physicochemical properties.…”

Section: Aminoglycosides Antibioticsmentioning

confidence: 99%

Floating resistivity detector for microchip electrophoresis

Tay¹,

Law²,

Sim³

et al. 2007

Electrophoresis

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A newly developed conductivity detector, the floating resistivity detector (FRD), for microchip electrophoresis was introduced in this work. The detector design permits decoupling of the detection circuit from the high separation voltage without compromising separation efficiency. This greatly simplifies the integration of microchip electrophoresis systems. Its method of detection relies on platinum electrodes being dipped in two buffer-filled branched detection probe reservoirs on the microchip device. In this way, analytes passing through the detection window will not pass through and subsequently adsorb onto the electrodes, alleviating problems of electrode fouling due to analyte contamination and surface reactions. A customized microchip design was proposed and optimized stepwise for the new FRD system. Each branched detection probe was determined to be 4.50 mm long with a 0.075 mm detection window gap between them. The distance between the detection window and buffer waste reservoir was determined to be 1.50 mm. The optimized microchip design was subsequently used in the analysis of four groups of analytes - inorganic cations, amino acids, aminoglycosides antibiotics, and biomarkers. Based on the preliminary results obtained, the detection limits were in the range of 0.4-0.7 mg/L for the inorganic cations and 1.5-15 mg/L for the amino compounds.

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“…Traditionally, antibiotics have been analyzed by nonspecific bioassay, but the methods allow interferences from active metabolites, degradants, and other antibiotics. Since aminoglycosidic antibiotic detection limits by short-wavelength UV detection are poor, the derivatization is often used to improve the detection properties of aminoglycosides [4][5][6], but its procedures increase sample preparation and limit analytes applicability to reactive compounds.…”

Section: Introductionmentioning

confidence: 99%

Characterization and photocatalytic activity of transition-metal-supported surface bond-conjugated TiO2/SiO2

Tang

2004

Catalysis Today

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Trace analysis of tobramycin in human plasma by derivatization and high-performance liquid chromatography with ultraviolet detection

Cited by 56 publications

References 26 publications

Formulation and Characterization of Lipid-Coated Tobramycin Particles for Dry Powder Inhalation

Formulation and Characterization of Lipid-Coated Tobramycin Particles for Dry Powder Inhalation

Floating resistivity detector for microchip electrophoresis

Characterization and photocatalytic activity of transition-metal-supported surface bond-conjugated TiO2/SiO2

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