Tracking Cancer Cells with Microfluidic High Frequency DEP Cytometer Implemented on BiCMOS Lab-on-Chip Platform

Manczak, Rémi; Hjeij, Fatima; Provent, Thomas; Saada, Sofiane; Dalmay, Claire; Bessette, Barbara; Begaud, G.; Battu, Serge; Blondy, Pierre; Jauberteau, Marie‐Odile; Lalloué, Fabrice; Inac, Mesut; Kaynak, Canan Baristiran; Kaynak, Mehmet; Palego, Cristiano; Pothier, A.

doi:10.1109/mwsym.2018.8439466

Cited by 3 publications

(2 citation statements)

References 9 publications

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“…By reducing analyte and reagent volumes and allowing for multiplexing, they have a large range of potential applications in chemical, bio-medical or environmental fields. In most lab-on-chip applications, effective control of temperature is a key factor in the optimization of the chip's performance, especially when biological samples are exposed to EMF [11,12]. Temperature measurements in a microfluidic channel, with very limited volume of analysis that include biological materials, requires the development of specific protocols as conventional physical probes are not suitable [13].…”

Section: Introductionmentioning

confidence: 99%

Rhodamine B Temperature Dosimetry of Biological Samples Interacting with Electromagnetic Fields in Macrosystems

Nefzi¹,

Carr²,

Dalmay³

et al. 2019

2019 IEEE MTT-S International Microwave Symposium (IMS)

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Exposing living cells to a certain level of Electromagnetic Field (EMF) might induce some biological effects including temperature elevation. In this paper, we show the dosimetry of exposure systems such as an Open Transverse Electro-Magnetic (TEM) cell allowing the study of the effect of EMF on biological samples exposed to 1.8 GHz signals.Temperature measurements are carried out with a fluorooptic probe to extract specific absorption rate (SAR) values that are compared to numerical dosimetry, based on a FDTD method. To investigate dosimetry at a microscopic level the fluorescence of the temperature dependent dye Rhodamine B was measured with fluorescence microscopy. The results are confirmed by measurements and simulations with a SAR of 13.9 and 11.8 W/kg for 1 W incident power, respectively. Results evidence that the objective working distance of the microscope strongly influence SAR values. After calibration, the fluorescence fits well with the temperature variation measured by the probe.

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Section: Introductionmentioning

confidence: 99%

Rhodamine B Temperature Dosimetry of Biological Samples Interacting with Electromagnetic Fields in Macrosystems

Nefzi¹,

Carr²,

Dalmay³

et al. 2019

2019 IEEE MTT-S International Microwave Symposium (IMS)

View full text Add to dashboard Cite

show abstract

“…By reducing analyte and reagent volumes and allowing for multiplexing, they have a large range of potential applications in chemical, biomedical, or environmental fields [14]. In most lab-on-chip applications, effective control of temperature is a key factor in the optimization of the chip's performance, especially when biological samples are exposed to RF [15], [16]. Under common conditions, RF power is limited to avoid significant heating [17].…”

mentioning

confidence: 99%

Microdosimetry Using Rhodamine B Within Macro- and Microsystems for Radiofrequency Signals Exposures of Biological Samples

Nefzi

Carr

Dalmay

et al. 2020

IEEE Trans. Microwave Theory Techn.

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Exposing living cells to a certain level of electromagnetic field (EMF) might induce some biological effects including temperature elevation. In this article, we studied two exposure systems at the macro and microscopic levels, allowing the study of the EMF effect on the biological samples exposed to 1.8-GHz signals. The macrosystem was an open transverse electromagnetic (TEM) cell that served as a dosimetry reference for defining limitations and optimal conditions for the temperature calibration using Rhodamine B (RhodB). The microfluidic microsystem was based on the coplanar waveguide (CPW) electrodes. Temperature measurements are carried out with a fluorooptic probe to extract specific absorption rate (SAR) values that are compared with numerical dosimetry, based on an FDTD method. After calibration, the fluorescence fits well with the temperature variation measured by the probe. To investigate dosimetry at a microscopic level, the fluorescence of the temperature-dependent dye RhodB was measured by fluorescence microscopy within the microfluidic channel or the biological cells. Results evidenced that the technique is applicable for RhodB concentrations higher than 1 µm with a value of 50 µm recommended for reliable experiments. For steady detection and SAR assessments, temperature variations of a few tenths of degrees were required.

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Validation of Clausius–Mossotti Function in Wideband Single-Cell Dielectrophoresis

et al. 2019

IEEE J. Electromagn. RF Microw. Med. Biol.

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Tracking Cancer Cells with Microfluidic High Frequency DEP Cytometer Implemented on BiCMOS Lab-on-Chip Platform

Cited by 3 publications

References 9 publications

Rhodamine B Temperature Dosimetry of Biological Samples Interacting with Electromagnetic Fields in Macrosystems

Rhodamine B Temperature Dosimetry of Biological Samples Interacting with Electromagnetic Fields in Macrosystems

Microdosimetry Using Rhodamine B Within Macro- and Microsystems for Radiofrequency Signals Exposures of Biological Samples

Validation of Clausius–Mossotti Function in Wideband Single-Cell Dielectrophoresis

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