Tracking mitochondrial <sup>1</sup>O<sub>2</sub>-ROS production through a differential mitochondria-nucleoli fluorescent probe

Flores‐Cruz, Ricardo; Jiménez‐Sánchez, Arturo

doi:10.1039/c8cc08289h

Cited by 18 publications

(5 citation statements)

References 21 publications

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“…Recent work has yielded additional probes to measure different ROS species in the mitochondria. RC334 was shown to selectively monitor mitochondrial singlet oxygen, 1 O 2 , without interference from pH and membrane potential, as well as other ROS species such as H 2 O 2 and OH radicals . Similarly, PBD-B is a ratiometric fluorescence reporter that selectively responds to superoxide over other ROS species over a wide range of concentrations both in vitro and in vivo .…”

Section: Mitochondrial Rosmentioning

confidence: 99%

Mitochondrial Targeting of Probes and Therapeutics to the Powerhouse of the Cell

Xia

2020

Bioconjugate Chem.

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Mitochondria, colloquially known as "the powerhouse of the cell", play important roles in production, but also in processes critical for cellular fate such as cell death, differentiation, signaling, metabolic homeostasis, and innate immunity. Due to its many functions in the cell, the mitochondria have been linked to a variety of human illnesses such as diabetes, cancer, and neurodegenerative diseases. In order to further our understanding and pharmaceutical targeting of this critical organelle, effective strategies must be employed to breach the complex barriers and microenvironment of mitochondria. Here, we summarize advancements in mitochondria-targeted probes and therapeutics.

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Section: Mitochondrial Rosmentioning

confidence: 99%

Mitochondrial Targeting of Probes and Therapeutics to the Powerhouse of the Cell

Xia

2020

Bioconjugate Chem.

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“…Even little changes of mitochondrial membrane potential could result a huge influence on the cell viability and are closely connected with a series of diseases. Therefore, development of sensitive methods to monitor mitochondrial membrane potential is particularly important 5–10…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporally Monitoring Cell Viability through Programmable Mitochondrial Membrane Potential Transformation by Using Fluorescent Carbon Dots

et al. 2020

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Mitochondria are the powerhouse of the cell, which maintain the metabolism of living cells. Mitochondrial membrane potential (MMP) is the key parameter of mitochondria and represents cellular activity. The programmable MMP transformations, i.e., normal, decrease, and vanishing, are indicative mitochondria healthy/damaged/dead states. Therefore, methods for monitoring MMP are of great importance. In this work, carbon dots responsive to mitochondrial membrane potential (MMP‐CDs) are prepared by a simple microwave method. The intracellular distribution of the MMP‐CDs is dependent on the mitochondrial membrane potential. Three different spatiotemporally organelle distributions (mitochondria/lysosome/nucleus) of the MMP‐CDs indicate three distinct MMP levels (normal/decrease/vanishing), which represent three different cell states (healthy/damaged/dead). The normal cells with high mitochondrial membrane potential causes the MMP‐CDs to accumulate in the mitochondria due to the Nernstian effect. The MMP‐CDs are released from the mitochondria and transported to the lysosome when cells are in apoptosis with the decreased mitochondrial membrane potential. In dead cells with a vanished mitochondrial membrane potential, the MMP‐CDs are collocated with the nucleus due to the affinity with DNA. Thus, various cellular states can be visualized through the different localizations of the MMP‐CDs. These MMP‐CDs are successfully employed in the detection of autophagy and H2O2‐induced mitochondria and mitochondrial membrane potential changes.

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“…Flores-Cruz et al 107 reported a fluorescent probe ( 25 ) to track the 1 O 2 generated in mitochondria through a unique mitochondria-to-nucleoli shuttle (Fig. 14C).…”

Section: Flavylium Based Fluorescent Probesmentioning

confidence: 99%

Natural flavylium-inspired far-red to NIR-II dyes and their applications as fluorescent probes for biomedical sensing

Sun

et al. 2022

Chem. Soc. Rev.

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Tracking mitochondrial ¹O₂-ROS production through a differential mitochondria-nucleoli fluorescent probe

Abstract: A dual-emissive fluorescent probe enabled unique tracking of 1O2-ROS species through a differential mitochondrial–nucleoli localization dynamic.

Cited by 18 publications

References 21 publications

Mitochondrial Targeting of Probes and Therapeutics to the Powerhouse of the Cell

Mitochondrial Targeting of Probes and Therapeutics to the Powerhouse of the Cell

Spatiotemporally Monitoring Cell Viability through Programmable Mitochondrial Membrane Potential Transformation by Using Fluorescent Carbon Dots

Natural flavylium-inspired far-red to NIR-II dyes and their applications as fluorescent probes for biomedical sensing

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Tracking mitochondrial 1O2-ROS production through a differential mitochondria-nucleoli fluorescent probe

Abstract: A dual-emissive fluorescent probe enabled unique tracking of 1O2-ROS species through a differential mitochondrial–nucleoli localization dynamic.

Cited by 18 publications

References 21 publications

Mitochondrial Targeting of Probes and Therapeutics to the Powerhouse of the Cell

Mitochondrial Targeting of Probes and Therapeutics to the Powerhouse of the Cell

Spatiotemporally Monitoring Cell Viability through Programmable Mitochondrial Membrane Potential Transformation by Using Fluorescent Carbon Dots

Natural flavylium-inspired far-red to NIR-II dyes and their applications as fluorescent probes for biomedical sensing

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Resources

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Tracking mitochondrial ¹O₂-ROS production through a differential mitochondria-nucleoli fluorescent probe