Tracking Red Blood Cells Flowing through a Microchannel with a Hyperbolic Contraction: An Automatic Method

Cell-free layer measurements ofin vitroblood flow in a microfluidic network: an automatic and manual approach

“…Although the manual methods can be extremely reliable they are highly time consuming and may introduce operator errors into the data. To overcome these drawbacks, several automatic and semi-automatic methods have been proposed to measure blood phenomena happening in microvessels and microfluidic devices (Sbalzarini & Koumoutsakos 2005;Pinho et al 2012;Yaginuma et al 2013;Taboada et al 2015). For instance, Pinho et al (2012) have developed a MATLAB module, based on a threshold algorithm, to track automatically RBCs flowing through a glass capillary.…”

“…For instance, Pinho et al (2012) have developed a MATLAB module, based on a threshold algorithm, to track automatically RBCs flowing through a glass capillary. More recently, Taboada et al (2015) have proposed an automatic image analysis technique, based on a keyhole algorithm, able to measure automatically both motion and deformability of individual RBCs. Although the existent automatic methods can perform automatic measurements,…”