Traditional in vitro strategies for sustainable production of bioactive compounds and manipulation of metabolomic profile in medicinal, aromatic and ornamental plants

Niazian, Mohsen; Sabbatini, Paolo

doi:10.1007/s00425-021-03771-5

Cited by 23 publications

(11 citation statements)

References 131 publications

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“…Based on the differential metabolites found by plant metabolomics, the network pharmacology can be used to predict the disease and target, and finally discover the therapeutic material basis and the predict the active mechanism of CMM. The relationship between different metabolites and their activity can be clarified by plant metabolomics combined with in vivo and in vitro efficacy experiments, then active markers of CMM can be discovered [ 106 – 110 ]. Ultimately, an associative network between environment-metabolite-efficacy will be established for a standardized cultivation-harvesting-processing system and a quality evaluation method of CMM.…”

Section: Concluding and Future Remarksmentioning

confidence: 99%

Plant metabolomics: a new strategy and tool for quality evaluation of Chinese medicinal materials

Xiao

Liu

et al. 2022

Chin Med

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The present quality control method of Chinese medicinal materials (CMM) has obvious deficiency, which cannot be compatible with the multi-target and multi-component characteristics and production process of CMM. Plant metabolomics with a huge impetus to comprehensively characterize the metabolites and clarify the complexity and integrity of CMM, has been widely used in the research of CMM. This article comprehensively reviewed the application of plant metabolomics in the quality control of CMM. It introduced the concept, technique, and application examples, discussed the prospects, limitations, improvements of plant metabolomics. MS and NMR, as important techniques for plant metabolomics, are mainly highlighted in the case references. The purpose of this article is to clarify the advantage of plants metabolomics for promoting the optimization of the CMM quality control system and proposing a system approach to realize the overall quality control of CMM based on plant metabolomics combined with multidisciplinary method.

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Section: Concluding and Future Remarksmentioning

confidence: 99%

Plant metabolomics: a new strategy and tool for quality evaluation of Chinese medicinal materials

Xiao

Liu

et al. 2022

Chin Med

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“…However, the use of potent synthetic chemicals during both onfield and post-harvest applications is progressively raising concerns regarding human health, environmental safety, and biodiversity. Consequently, there is a growing need to explore the potential of microorganisms or plant-derived bioactive secondary Microorganisms 2024, 12, 184 2 of 15 metabolites as environmentally friendly alternatives for the development of fungicides to control phytopathogenic P. litchii [6,7].…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Volatiles Emitted by Streptomyces abikoensis TJGA-19 for Managing Litchi Downy Blight Disease

Xing,

Sun,

Liu

et al. 2024

Microorganisms

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Litchi is a fruit of significant commercial value; however, its quality and yield are hindered by downy blight disease caused by Peronophythora litchii. In this study, volatile organic compounds (VOCs) from Streptomyces abikoensis TJGA-19 were investigated for their antifungal effects and studied in vitro and in planta for the suppression of litchi downy blight disease in litchi leaves and fruits. The growth of P. litchii was inhibited by VOCs produced by TJGA-19 cultivated on autoclaved wheat seeds for durations of 10, 20, or 30 days. Volatiles from 20-day-old cultures were more active in inhibition effect against P. litchii than those from 10- or 30-day-old cultures. These volatiles inhibit the growth of mycelia, sporulation, and oospore production, without any significant effect on sporangia germination. Additionally, the VOCs were effective in suppressing disease severity in detached litchi leaf and fruit infection assays. With the increase in the weight of the wheat seed culture of S.abikoensis TJGA-19, the diameters of disease spots on leaves, as well as the incidence rate and disease indices on fruits, decreased significantly. Microscopic results from SEM and TEM investigations showed abnormal morphology of sporangia, mycelia, and sporangiophores, as well as organelle damage in P. litchii caused by VOCs of TJGA-19. Spectroscopic analysis revealed the identification of 22 VOCs produced by TJGA-19, among which the most dominant compound was 2-Methyliborneol. These findings indicated the significant role of TJGA-19 compounds in the control of litchi downy blight disease and in improving fruit quality.

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“…Plant tissue culture has been widely utilized for the biosynthesis of several plant-derived metabolites such as catharanthine [ 8 ], chamazulene [ 9 ], diosgenin [ 10 ], ginsenosides [ 11 ], paclitaxel [ 12 ], rosmarinic acid [ 13 ], shikonin [ 14 ], and tropane alkaloids [ 15 ]. In vitro systems, also known as “cell factories’’, facilitate the production of desirable phytochemical compounds, manipulating metabolic pathways by shifting culture conditions such as media components, environmental parameters, growth regulators, and elicitors [ 9 , 16 , 17 , 18 ]. Furthermore, callus cultures are considered a sustainable and environmentally friendly method for plant biomass production, without any requirements for arable land and/or negative effects on ecosystems and biodiversity [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Farm or Lab? A Comparative Study of Oregano’s Leaf and Callus Volatile Isolates Chemistry and Cytotoxicity

Kakalis

Tsekouras

Mavrikou

et al. 2023

Plants

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Oregano (Origanum vulgare, Lamiaceae plant family) is a well-known aromatic herb with great commercial value, thoroughly utilized by food and pharmaceutical industries. The present work regards the comparative assessment of in vitro propagated and commercially available oregano tissue natural products. This study includes their secondary metabolites’ biosynthesis, antioxidant properties, and anticancer activities. The optimization of callus induction from derived oregano leaf explants and excessive oxidative browning was performed using various plant growth regulators, light conditions, and antioxidant compounds. The determination of oregano callus volatiles against the respective molecules in maternal herbal material was performed using gas chromatography–mass spectrometry (GC/MS) analysis. In total, the presence of twenty-seven phytochemicals was revealed in both leaf and callus extracts, from which thirteen molecules were biosynthesized in both tissues studied, seven compounds were present only in callus extracts, and seven metabolites only in leaf extracts. Carvacrol and sabinene hydrate were the prevailing volatiles in all tissues exploited, along with alkanes octacosane and triacontane and the trimethylsilyl (TMS) derivative of carvacrol that were detected in significant amounts only in callus extracts. The MTT assay was employed to assess the in vitro cytotoxic properties of oregano extracts against the epithelial human breast cancer MDA-MB-231 and the human neuroblastoma SK-N-SH cell lines. The extracts displayed concentration and time-dependent responses in cell proliferation rates.

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Traditional in vitro strategies for sustainable production of bioactive compounds and manipulation of metabolomic profile in medicinal, aromatic and ornamental plants

Cited by 23 publications

References 131 publications

Plant metabolomics: a new strategy and tool for quality evaluation of Chinese medicinal materials

Plant metabolomics: a new strategy and tool for quality evaluation of Chinese medicinal materials

Effectiveness of Volatiles Emitted by Streptomyces abikoensis TJGA-19 for Managing Litchi Downy Blight Disease

Farm or Lab? A Comparative Study of Oregano’s Leaf and Callus Volatile Isolates Chemistry and Cytotoxicity

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