1987
DOI: 10.1128/mcb.7.8.2753
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Transcription of the constitutively expressed yeast enolase gene ENO1 is mediated by positive and negative cis-acting regulatory sequences.

Abstract: There are two enolase genes, ENO] and EN02, per haploid yeast genome. Expression of the ENO] gene is quantitatively similar in cells grown on glucose or gluconeogenic carbon sources. In contrast, EN02 expression is induced more than 20-fold in cells grown on glucose as the carbon source. cis-Acting regulatory sequences were mapped within the 5'-flanking region of the constitutively expressed yeast enolase gene ENO]. A complex positive regulatory region was located 445 base pairs (bp) upstream from the transcri… Show more

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Cited by 84 publications
(83 citation statements)
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“…DNA fragments containing these sequences were demonstrated to possess two characteristics expected of a repressor binding site as described above. In (2,11). These results are consistent with our interpretation.…”
Section: Discussionsupporting
confidence: 83%
“…DNA fragments containing these sequences were demonstrated to possess two characteristics expected of a repressor binding site as described above. In (2,11). These results are consistent with our interpretation.…”
Section: Discussionsupporting
confidence: 83%
“…The dual regulation of a single enolase gene in A. nidulans contrasts with the presence of two genes in S. cerevisiae. ENO2 is 20-fold induced in the presence of glucose while ENO1 is equally expressed on glucose or gluconeogenic carbon sources (Cohen et al 1987).…”
Section: Discussionmentioning
confidence: 99%
“…On the other hand, the expression of GPM1 is constitutive (Rodicio et al, 1993). In the case of enolase, expression of ENO1 is constitutive, but the expression of ENO2 is glucose-inducible (Cohen et al, 1986;Cohen et al, 1987;McAlister and Holland, 1985). However, in our wild-type strain 2845, the Glc : GL ratios of Pgk, Gpm, Eno, Pyk and Zwf (a control non-glycolytic enzyme) enzyme activities were 1.05, 1.27, 2.20, 2.46 and 0.70, respectively (Table 5, lines 1-3).…”
Section: Regulation Of Gcr1 Expression Bymentioning
confidence: 99%
“…Whether their expression is regulated may be strain-dependent, with reports ranging from virtual constitutivity (Baker, 1986;Clifton and Fraenkel, 1981;Uemura and Fraenkel, 1999) to apparent induction by glucose of at least some of the enzymes (Chambers et al, 1989;Cohen et al, 1987;Maitra and Lobo, 1971;Nishizawa et al, 1989). Since many of the steps of glycolysis are shared by gluconeogenesis, most glycolytic genes, including those with glucose induction, are also highly expressed in media containing non-fermentable carbon sources.…”
Section: Introductionmentioning
confidence: 99%