Transcriptional Activation of Anthocyanin Biosynthesis in Developing Fruit of Blueberries (<i>Vaccinium corymbosum</i> L.) by Preharvest and Postharvest UV Irradiation

Jiao, Yang; Li, Binbin; Shi, Wenjun; Gong, Zhizhong; Chen, Lu; Hou, Zhixia

doi:10.1021/acs.jafc.8b03081

Cited by 52 publications

(30 citation statements)

References 60 publications

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“…UV-B, C irradiation significantly promoted anthocyanin biosynthesis and the transcripts of anthocyanin downstream pathway genes ( DFR , ANR , and UFGT ) as well as DFR and UFGT enzymatic activities in blueberries [20]. In our study, flavonols content was positively correlated with the transcripts of downstream genes ( LAR , ANR , and LDOX ) as well as initiative upstream genes ( PAL and C4H ) in flavonoid biosynthetic pathway responding to cold stress and coincided with flavonols biosynthesis.…”

Section: Discussionsupporting

confidence: 57%

Transcriptome profiling reveals candidate flavonol-related genes of Tetrastigma hemsleyanum under cold stress

Peng

Chen

et al. 2019

BMC Genomics

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Background Tetrastigma hemsleyanum Diels et Gilg is a valuable medicinal herb, whose main bioactive constituents are flavonoids. Chilling sensitivity is the dominant environmental factor limiting growth and development of the plants. But the mechanisms of cold sensitivity in this plant are still unclear. Also, not enough information on genes involved in flavonoid biosynthesis in T. hemsleyanum is available to understand the mechanisms of its physiological and pharmaceutical effects. Results The electrolyte leakage, POD activity, soluble protein, and MDA content showed a linear sustained increase under cold stress. The critical period of cold damage in T. hemsleyanum was from 12 h to 48 h. Expression profiles revealed 18,104 differentially expressed genes (DEGs) among these critical time points. Most of the cold regulated DEGs were early-response genes. A total of 114 unigenes were assigned to the flavonoid biosynthetic pathway. Fourteen genes most likely to encode flavonoid biosynthetic enzymes were identified. Flavonols of T. hemsleyanum might play a crucial role in combating cold stress. Genes encoding P AL, 4CL , CHS, ANR, FLS , and LAR were significantly up-regulated by cold stress, which could result in a significant increase in crucial flavonols (catechin, epicatechin, rutin, and quercetin) in T. hemsleyanum. Conclusions Overall, our results show that the expression of genes related to flavonol biosynthesis as well as flavonol content increased in T. hemsleyanum under cold stress. These findings provide valuable information regarding the transcriptome changes in response to cold stress and give a clue for identifying candidate genes as promising targets that could be used for improving cold tolerance via molecular breeding. The study also provides candidate genes involved in flavonoid biosynthesis and may be useful for clarifying the biosynthetic pathway of flavonoids in T. hemsleyanum . Electronic supplementary material The online version of this article (10.1186/s12864-019-6045-y) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 57%

Transcriptome profiling reveals candidate flavonol-related genes of Tetrastigma hemsleyanum under cold stress

Peng

Chen

et al. 2019

BMC Genomics

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“…In contrast, UV‐B induced anthocyanin production is mainly caused by the activation of downstream biosynthetic genes and MYB TFs, as shown in apple, blueberry ( Vaccinium corymbosum L.) and grape berries ( V. vinifera × V. labrusca cv. Summer Black) (Henry‐Kirk et al ., ; Yang et al ., ; Sheng et al ., ). Our results suggest that the miR156a−MLNC3.2 and MLNC4.6− SPL2‐like and SPL33 network is not involved in anthocyanin accumulation in response to UV‐B treatment, and that the anthocyanin accumulation in UV‐B treated apple fruit results from the expression of SPL TFs.…”

Section: Discussionmentioning

confidence: 99%

Systematic identification of long noncoding RNAs expressed during light‐induced anthocyanin accumulation in apple fruit

et al. 2019

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Summary Anthocyanin pigments contribute to the red color of apple (Malus × domestica) fruit and have a major influence on their ornamental, dietary and market value. In this study, we investigated the potential role of long noncoding RNAs (lncRNAs) in anthocyanin biosynthesis. RNA‐seq analysis of apple peels from the ‘Red Fuji’ cultivar during light‐induced rapid anthocyanin accumulation revealed 5297 putative lncRNAs. Differential expression analysis further showed that lncRNAs were induced during light treatment and were involved in photosynthesis. Using the miRNA−lncRNA−mRNA network and endogenous target mimic (eTM) analysis, we predicted that two differentially expressed lncRNAs, MLNC3.2 and MLNC4.6, were potential eTMs for miRNA156a and promoted the expression of the SPL2‐like and SPL33 transcription factors. Transient expression in apple fruit and stable transformation of apple callus showed that overexpression of the eTMs and SPLs promoted anthocyanin accumulation, with the opposite results in eTM and SPL‐silenced fruit. Silencing or overexpressing of miR156a also affected the expression of the identified eTMs and SPLs. These results indicated that MLNC3.2 and MLNC4.6 function as eTMs for miR156a and prevent cleavage of SPL2‐like and SPL33 by miR156a during light‐induced anthocyanin biosynthesis. Our study provides fundamental insights into lncRNA involvement in the anthocyanin biosynthetic pathway in apple fruit.

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“…Anthocyanins have been demonstrated to not only affect the color formation of fruit, but also can improve the antioxidant capacity and prevent a variety of diseases such as cancer [2]. Therefore, to date, most studies on blueberries have focused on the research of anthocyanin formation and regulation mechanism [3,4]. However, in recent years, with global warming and frequency of adversity [5], the survival of blueberry tree is often threatened by drought stress, and the output is further affected.…”

Section: Introductionmentioning

confidence: 99%

Identification and Expression of NAC Transcription Factors of Vaccinium corymbosum L. in Response to Drought Stress

Liang

Wang

Sun

et al. 2019

Forests

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Research Highlights: Phenotypic changes and expression profiles, phylogeny, conserved motifs, and expression correlations of NAC (NAM, ATAF1, ATAF2 and CUC2) transcription factors (TFs) in blueberry genome were detected under drought stress, and the expression patterns and functions of 12 NACs were analyzed. Background and Objectives: Blueberry is an important shrub species with a high level of flavonoids in fruit, which are implicated in a broad range of health benefits. However, the molecular mechanism of this shrub species in response to drought stress still remains elusive. NAC TFs widely participate in stress tolerance in many plant species. The characterization and expression profiles of NAC TFs were analyzed on the basis of genome data in blueberry when subjected to drought stress. Materials and Methods: Combined with the analysis of chlorophyll a fluorescence and endogenous phytohormones, the phenotypic changes of blueberry under drought stress were observed. The phylogenetic tree, conserved motifs, differently expressed genes, and expression correlation were determined by means of multiple bioinformatics analysis. The expression profiles of NACs in different organs were examined and compared through RNA-seq and qRT-PCR assay. Results: The chlorophyll a fluorescence parameters φPo, φEo, φRo, and PIabs of leaves were significantly inhibited under drought stress. ABA (abscisic acid) content noticeably increased over the duration of drought, whereas GA3 (gibberellic acid) and IAA (indole acetic acid) content decreased continuously. A total of 158 NACs were identified in blueberry genome and 62 NACs were differently expressed in leaf and root of blueberry under drought stress. Among them, 14 NACs were significantly correlated with the expression of other NAC genes. Conclusions: Our results revealed the phenotypic changes of this shrub under drought stress and linked them with NAC TFs, which are potentially involved in the process of response to drought stress.

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Transcriptional Activation of Anthocyanin Biosynthesis in Developing Fruit of Blueberries (Vaccinium corymbosum L.) by Preharvest and Postharvest UV Irradiation

Cited by 52 publications

References 60 publications

Transcriptome profiling reveals candidate flavonol-related genes of Tetrastigma hemsleyanum under cold stress

Transcriptome profiling reveals candidate flavonol-related genes of Tetrastigma hemsleyanum under cold stress

Systematic identification of long noncoding RNAs expressed during light‐induced anthocyanin accumulation in apple fruit

Identification and Expression of NAC Transcription Factors of Vaccinium corymbosum L. in Response to Drought Stress

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