Transcriptional and Physiological Responses of
            <i>Bradyrhizobium japonicum</i>
            to Desiccation-Induced Stress

Cytryn, Eddie; Sangurdekar, Dipen; Streeter, John G.; Franck, William L.; Chang, Woo Suk; Stacey, Gary; Emerich, David W.; Joshi, Trupti; Xu, Dong; Sadowsky, Michael J.

doi:10.1128/jb.01543-07

Cited by 58 publications

(98 citation statements)

References 0 publications

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“…Indeed, studying Saccharomyces cerevisiae, Benaroudj et al (2001) showed that in addition to its structural function in desiccation tolerance, trehalose accumulates during cellular stress (exposure to mild heat shock at 38 1C or to a proteasome inhibitor) and markedly protects cells and proteins from damage generated from an oxygen radical-generating system (that is, H 2 O 2 and FeCl 3 through the Fenton reaction). A similar role was suggested by Cytryn et al (2007) who studied the desiccation-induced stress in the a-proteobacterium Bradyrhizobium japonicum.…”

Section: Discussionmentioning

confidence: 80%

Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight

et al. 2009

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In view of long-haul space exploration missions, the European Space Agency initiated the MicroEcological Life Support System Alternative (MELiSSA) project targeting the total recycling of organic waste produced by the astronauts into oxygen, water and food using a loop of bacterial and higher plant bioreactors. In that purpose, the a-proteobacterium, Rhodospirillum rubrum S1H, was sent twice to the International Space Station and was analyzed post-flight using a newly developed R. rubrum whole genome oligonucleotide microarray and high throughput gel-free proteomics with Isotope-Coded Protein Label technology. Moreover, in an effort to identify a specific response of R. rubrum S1H to space flight, simulation of microgravity and space-ionizing radiation were performed on Earth under identical culture set-up and growth conditions as encountered during the actual space journeys. Transcriptomic and proteomic data were integrated and permitted to put forward the importance of medium composition and culture set-up on the response of the bacterium to space flight-related environmental conditions. In addition, we showed for the first time that a low dose of ionizing radiation (2 mGy) can induce a significant response at the transcriptomic level, although no change in cell viability and only a few significant differentially expressed proteins were observed. From the MELiSSA perspective, we could argue the effect of microgravity to be minimized, whereas R. rubrum S1H could be more sensitive to ionizing radiation during long-term space exploration mission.

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Section: Discussionmentioning

confidence: 80%

Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight

et al. 2009

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“…However, we did not detect TreS activity when the organism was grown on maltose. It is also possible that these pathways may be activated by other environmental stimuli such as desiccation, which induces Tps/Tpp and TreS in Bradyrhizobium japonicum (6).…”

Section: Discussionmentioning

confidence: 99%

“…Cells carrying pET30b-tps, pET30b-tpp, or pET11a-treT were grown to an optical density at 610 nm of 1.0 at 37°C in LB medium (2 liters) containing kanamycin (30 g/ml) or ampicillin (100 g/ml) for selection, and IPTG (isopropyl-␤-D-thiogalactopyranoside) (1 mM) was added to induce gene expression. Cells carrying (His) 6 -Tps and (His) 6 -Tpp were allowed to grow for ϳ6 h, harvested, and suspended in 20 mM sodium phosphate buffer with 0.5 M NaCl and 10 mM MgCl 2 , DNase (4 g/ml), and a protease inhibitor cocktail (Roche). Cells carrying the recombinant TreT were allowed to grow for a further 10 h at 30°C, suspended in 20 mM Tris-HCl at pH 7.6, and disrupted in a French pressure cell, and debris was removed by centrifugation.…”

Section: Methodsmentioning

confidence: 99%

A Unique Combination of Genetic Systems for the Synthesis of Trehalose in Rubrobacter xylanophilus : Properties of a Rare Actinobacterial TreT

et al. 2008

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Trehalose is the primary organic solute inRubrobacter xylanophilus under all conditions tested, including those for optimal growth. We detected genes of four different pathways for trehalose synthesis in the genome of this organism, namely, the trehalose-6-phosphate synthase (Tps)/trehalose-6-phosphate phosphatase (Tpp), TreS, TreY/TreZ, and TreT pathways. Moreover, R. xylanophilus is the only known member of the phylum Actinobacteria to harbor TreT. The Tps sequence is typically bacterial, but the Tpp sequence is closely related to eukaryotic counterparts. Both the Tps/Tpp and the TreT pathways were active in vivo, while the TreS and the TreY/TreZ pathways were not active under the growth conditions tested and appear not to contribute to the levels of trehalose observed. The genes from the active pathways were functionally expressed in Escherichia coli, and Tps was found to be highly specific for GDP-glucose, a rare feature among these enzymes. The trehalose-6-phosphate formed was specifically dephosphorylated to trehalose by Tpp. The recombinant TreT synthesized trehalose from different nucleoside diphosphate-glucose donors and glucose, but the activity in R. xylanophilus cell extracts was specific for ADP-glucose. The TreT could also catalyze trehalose hydrolysis in the presence of ADP, but with a very high K m . Here, we functionally characterize two systems for the synthesis of trehalose in R. xylanophilus, a representative of an ancient lineage of the actinobacteria, and discuss a possible scenario for the exceptional occurrence of treT in this extremophilic bacterium.

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“…Microarray analysis of B. japonicum USDA110 in response to desiccation stress showed that a number of genes responsible for proteins involved in membrane protection, repair of DNA damage, stability of proteins and oxidative stress reduction were upregulated when compared to hydrated cells (Cytryn et al, 2007). They also reported that the number of genes upregulated by osmotic stress were significantly fewer than those upregulated by desiccation stress.…”

Section: Desiccation Tolerance Mechanismsmentioning

confidence: 95%

“…A study reporting on the response of B. japonicum USDA 110 to desiccation stress identified several genes related to synthesis of LPS that are upregulated in response to desiccation (Cytryn et al, 2007). The LPS of R. leguminosarum bv.…”

Section: Desiccation Tolerance Mechanismsmentioning

confidence: 99%

The Physiological Mechanisms of Desiccation Tolerance in Rhizobia

Casteriano¹,

Wilkes²,

Deaker³

2015

Biological Nitrogen Fixation

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Transcriptional and Physiological Responses of Bradyrhizobium japonicum to Desiccation-Induced Stress

Cited by 58 publications

References 0 publications

Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight

Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight

A Unique Combination of Genetic Systems for the Synthesis of Trehalose in Rubrobacter xylanophilus : Properties of a Rare Actinobacterial TreT

The Physiological Mechanisms of Desiccation Tolerance in Rhizobia

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