Transcriptional Control of the Isoeugenol Monooxygenase ofPseudomonas nitroreducensJin1 inEscherichia coli

Isomerization of Allylbenzenes

“…Scientific investigation concerning biotransformation of naturally occurring, and thus potentially renewable, feedstock has become an active area of research. Of particular relevance to this Review are recent publications on the value of eugenol 5 , isoeugenol 18 , and related compounds in this regard. , In addition, the list of microorganisms able to biotransform these substrates is increasing, due to new discoveries being made. − Our deeper knowledge and understanding of the workings concerning the genes found in these organisms responsible for these valuable natural reactions will undoubtedly drastically increase over time. − With this as current background, we are quite convinced that a new area of innovative research that will impact the application of isomerization transformations will emanate from research into microorganism-mediated biotransformations.…”

Bacterial biotransformation of phenylpropanoid compounds for producing flavor and fragrance compounds

“…The transcription of iem was significantly increased with overproduction of IemR in E. coli , up to 96-fold, even in the absence of isoeugenol, as compared to basally expressed IemR, an indication that the transcription of iem is dependent on the type of inducers and on IemR. This basic research suggest that the development of bioengineering strategies for increased production of vanillin through high-level expression of the isoeugenol monooxygenase gene in microorganisms (Ryu et al, 2012) could be possible. Furthermore, isoeugenol monooxygenase (Iem) was overexpressed in E. coli and purified.…”

“…7 Biotransformation of and gene arrangement for isoeugenol to vanillin by isoeugenol monooxygenase of P. nitroreducens Jin1. Biotransformation pathway of isoeugenol to vanillin by isoeugenol monooxygenase (Iem) (A), the gene arrangement (B) and predicted promoter sequence (C) of isoeugenol monooxygenase regulator (iemR) and iem genes (B) (adapted from Ryu et al, 2012). Fig.…”

Vanillin–Bioconversion and Bioengineering of the Most Popular Plant Flavor and Its De Novo Biosynthesis in the Vanilla Orchid

“…IemR was suggested to be a positive transcriptional regulator of Iem. It was suggested that the high level expression and use of multiple copies of Iem and IemR resulted in increased production of vanillin (Ryu et al, 2012) by increasing the metabolic flux toward vanillin.…”

“…In 1966, glucose-induced vanillin formation in soils was reported (Kunc and Macura, 1966). This phenomenon was suggested to be due to soil bacteria such as Pseudomonas strains metabolizing glucose to vanillin and vanillic acid (Ryu et al, 2012). However, no direct evidence was provided to demonstrate that the vanillin observed was actually synthesized from glucose by the Pseudomonas studied.…”