Transcriptional mapping of rabies virus in vivo

Flamand, Anne; Delagneau, J.F.

doi:10.1128/jvi.28.2.518-523.1978

Cited by 50 publications

(17 citation statements)

References 15 publications

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“…Thus, the gene order of the VSV genome RNA is 3' leader RNA-N-NS-M-G-L 5' (1, 7), which was subsequently confirmed by Reddy et al (177) and by electron microscopic analysis (96). By similar UV transcriptional mapping studies, the gene order of rabies virus was established as (3') leader RNA-N-M1-M2-G-L 5' (75). It is of interest to note that a fish rhabdovirus, spring viremia of carp virus, appears to have the matrix protein gene (M) next to N protein gene rather than the phosphoprotein gene (NS) as found in other rhabdoviruses (P. Roy, personal communication).…”

Section: Uv Transcriptional Mapping and Gene Ordermentioning

confidence: 99%

Transcription and replication of rhabdoviruses.

Banerjee¹

1987

Microbiological Reviews

187

110

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Section: Uv Transcriptional Mapping and Gene Ordermentioning

confidence: 99%

Transcription and replication of rhabdoviruses.

Banerjee¹

1987

Microbiological Reviews

187

110

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“…Clearly, this numeration gives no indication about the relative amount of mRNA species. It is probable that some are more abundant than the others, since the five viral cistrons may not be transcribed with the same efficiency, depending on their position on the viral genome (9,14).…”

Section: Resultsmentioning

confidence: 99%

Biochemical characterization of temperature-sensitive rabies virus mutants

Saghi¹,

Flamand²

1979

J Virol

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Biochemical characterization of 70 temperature-sensitive (ts) mutants of rabies virus has been done by following the appearance of viral proteins and RNA molecules in infected cells at both permissive and nonpermissive temperature. The presence or absence of the nucleocapsid protein (N) was demonstrated by treating infected cells with anti-N fluorescent antibodies. At 33 degrees C, all the mutants induced a fluorescence comparable to the wild type. At 39.6 degrees C, the mutants can be classified into three groups. Three mutants induced a fluorescence comparable to the wild type (F+ mutants); 54 mutants induced a faint fluorescence which was proportional to the multiplicity of infection and increased with time (F+- mutants). No fluorescence could be detected for the 13 remaining mutants (F- mutants). The synthesis of all viral proteins was shown to be normal for F+ mutants, indicating that transcription and replication of the virus were normal and that the ts lesion was located in a protein which is not directly required for those functions. The synthesis of all viral proteins was similarly decreased for F+- mutants and undetectable for the F- mutants. This suggests that the ts lesion affects the transcription and/or replication of the virus. By annealing techniques it was demonstrated that the F+- mutants were able to perform some amount of secondary transcription at nonpermissive temperature. No secondary transcription occurred with F- mutants. When detectable (i.e., at higher multiplicity of infection), primary transcription of F- mutants was normal.

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“…This may be due to attenuation of transcription at or near the intergenic regions (9,19). However, it has not been definitely established whether rabies virus mRNA synthesis is initiated only at the 3' end of the genomic RNA by the RNA polymerase to sequentially produce transcripts of the downstream genes, as described by Flamand and Delagneau (7). Alternatively, multiple initiations might occur at the different gene start sites, which has been proposed as a mechanism for vesicular stomatitis virus transcription (2).…”

Section: Discussionmentioning

confidence: 99%

Detection of rabies virus genomic RNA and mRNA in mouse and human brains by using in situ hybridization

Jackson

Wunner

1991

J Virol

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Rabies virus RNA was detected in mouse and human brains by in situ hybridization. 3H-labeled single-stranded RNA probes were prepared which were specific for genomic RNA and mRNAs coding for the five rabies virus proteins (N, NS, M, G, and L). Paraffin-embedded brain tissues from human cases of rabies and mice experimentally infected with the challenge virus standard (CVS)-11 strain of rabies virus and street rabies virus were examined. In CVS-infected mice, genomic RNA had a multifocal distribution in the perikarya of infected neurons, perhaps reflecting concentration of genomic RNA in viral factories. The mRNAs were more abundant than genomic RNAs in CVSand street virus-infected mouse brains and had a diffuse distribution in the perikarya. Similar amounts of signal were present in infected neurons for mRNAs coding for different rabies virus proteins. In brain tissues from human cases of rabies, genomic RNA was much more abundant than the mRNAs in infected neurons. This finding suggests either a relative block at the level of transcription or greater loss of mRNAs than of genomic RNA during the agonal period, postmortem interval, or prior to penetration of fixative during immersion fixation.

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Transcriptional mapping of rabies virus in vivo

Cited by 50 publications

References 15 publications

Transcription and replication of rhabdoviruses.

Transcription and replication of rhabdoviruses.

Biochemical characterization of temperature-sensitive rabies virus mutants

Detection of rabies virus genomic RNA and mRNA in mouse and human brains by using in situ hybridization

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