Transcriptional modulation of some Staphylococcus aureus iron-regulated genes during growth in vitro and in a tissue cage model in vivo

Allard, Marie-Julie; Moisan, Hélène; Brouillette, Éric; Gervais, Alain; Jacques, Mario; Lacasse, P.; Diarra, Moussa S.; Malouin, François

doi:10.1016/j.micinf.2006.01.022

Cited by 57 publications

(50 citation statements)

References 27 publications

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“…Specifically, we show that Fur affects the reciprocal expression of secreted cytolysins and a subset of immunomodulatory factors, which supports the hypothesis that iron-starved S. aureus expresses cytolysins, whereas staphylococci with sufficient iron secrete numerous immunomodulatory factors. The pathophysiological relevance of this coordinated expression program is highlighted by the observation that S. aureus lacking Fur is less virulent in a murine model of pneumonia and by the fact that the differential expression of staphylococcal virulence factors has also been observed in vivo (2). Despite extensive studies of other organisms, this is the first report of the global impact of Fur on staphylococcal exoprotein production.…”

Section: Discussionmentioning

confidence: 85%

“…Recently, it was demonstrated that S. aureus senses iron limitation to alter the expression of iron uptake systems, as well as the expression of a series of virulence factors (2). S. aureus is known to monitor iron availability via Fur (25,32,41,66,71); however, the global contribution of iron and Fur to staphylococcal exoprotein production has not been evaluated.…”

Section: Fur Alters the Production Of Staphylococcal Exoproteinsmentioning

confidence: 99%

“…These signals include, but are not limited to, changes in nutrient availability, temperature, pH, osmolarity, and oxygen tension. S. aureus senses these and other cues to alter the expression of virulence factors (2,11,12,15,26,37,50,56,69); however, the molecular mechanisms employed by staphylococci to sense signals present in thejr hosts are not well understood.…”

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confidence: 99%

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Staphylococcus aureus Fur Regulates the Expression of Virulence Factors That Contribute to the Pathogenesis of Pneumonia

Torres¹,

Attia

Mason³

et al. 2010

Infect Immun

137

115

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The tremendous success of Staphylococcus aureus as a pathogen is due to the controlled expression of a diverse array of virulence factors. The effects of host environments on the expression of virulence factors and the mechanisms by which S. aureus adapts to colonize distinct host tissues are largely unknown. Vertebrates have evolved to sequester nutrient iron from invading bacteria, and iron availability is a signal that alerts pathogenic microorganisms when they enter the hostile host environment. Consistent with this, we report here that S. aureus senses alterations in the iron status via the ferric uptake regulator (Fur) and alters the abundance of a large number of virulence factors. These Fur-mediated changes protect S. aureus against killing by neutrophils, and Fur is required for full staphylococcal virulence in a murine model of infection. A potential mechanistic explanation for the impact of Fur on virulence is provided by the observation that Fur coordinates the reciprocal expression of cytolysins and a subset of immunomodulatory proteins. More specifically, S. aureus lacking fur exhibits decreased expression of immunomodulatory proteins and increased expression of cytolysins. These findings reveal that Fur is involved in initiating a regulatory program that organizes the expression of virulence factors during the pathogenesis of S. aureus pneumonia.

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Section: Discussionmentioning

confidence: 85%

Section: Fur Alters the Production Of Staphylococcal Exoproteinsmentioning

confidence: 99%

mentioning

confidence: 99%

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Staphylococcus aureus Fur Regulates the Expression of Virulence Factors That Contribute to the Pathogenesis of Pneumonia

Torres¹,

Attia

Mason³

et al. 2010

Infect Immun

137

115

View full text Add to dashboard Cite

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“…NWMN2274 is in a predicted two-gene operon with NWMN2273 that encodes a putative GCN5-like N-acetyltransferase. The 231-base pair intergenic region between NWMN2274 and NWMN2275 includes probable Ϫ10 and Ϫ35 sites for RNA polymerase binding and a predicted Fur box identical to that identified by Allard et al (39) for SA2162 (Fig. 1B).…”

Section: Identification Of Nwmn2274 As a Putative Electron Donor Tomentioning

confidence: 56%

“…Using a combination of cell culture and infection models with a bovine mastitis S. aureus isolate (strain SHY97-3906), Allard et al (39) found that SACOL2369 was up-regulated under both iron-restricted growth conditions in cell culture and in tissue cages embedded in mice abdomens. SACOL2369 is annotated as a pyridine nucleotide-disulfide oxidoreductase (PNDO), and the authors of this study confirmed the microarray results by real time PCR.…”

Section: Identification Of Nwmn2274 As a Putative Electron Donor Tomentioning

confidence: 99%

IruO Is a Reductase for Heme Degradation by IsdI and IsdG Proteins in Staphylococcus aureus

Loutet¹,

Kobylarz

Chau

et al. 2013

Journal of Biological Chemistry

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Quantitative proteomes and in vivo secretomes of progressive and regressive UV‐induced fibrosarcoma tumor cells: Mimicking tumor microenvironment using a dermis‐based cell‐trapped system linked to tissue chamber

et al. 2007

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The alterations of tumor proteome and/or in vivo secretome created by host-tumor cell interaction may be crucial factors for tumors to undergo progression or regression in a host system. Two UV-induced fibrosarcoma tumor cell lines (UV-2237 progressive cells and UV-2240 regressive cells) were used as models to address this issue. Hundreds of proteins including in vivo secretome have been identified and quantified via an isotope-coded protein label (ICPL) in conjunction with high-throughput NanoLC-LTQ MS analysis. A newly designed technology using a dermis-based cell-trapped system was employed to encapsulate and grow 3-D tumor cells. A tissue chamber inserted with a tumor cell-trapped dermis was implanted into mice to mimic the tumor microenvironment. The in vivo secretome created by host-tumor interaction was characterized from samples collected from tissue chamber fluids via ICPL labeling mass spectrometric analysis. Twenty-five proteins including 14-3-3 proteins, heat shock proteins, profilin-1, and a fragment of complement C3 with differential expression in proteomes of UV-2237 and UV-2240 cells were revealed. Three secreted proteins including myeloperoxidase, alpha-2-macroglobulin, and a vitamin D-binding protein have different abundances in the in vivo secretome in response to UV-2237 and UV-2240 cells. Differential tumor proteomes and in vivo secretome were thus accentuated as potential therapeutic targets to control tumor growth.

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Transcriptional modulation of some Staphylococcus aureus iron-regulated genes during growth in vitro and in a tissue cage model in vivo

Cited by 57 publications

References 27 publications

Staphylococcus aureus Fur Regulates the Expression of Virulence Factors That Contribute to the Pathogenesis of Pneumonia

Staphylococcus aureus Fur Regulates the Expression of Virulence Factors That Contribute to the Pathogenesis of Pneumonia

IruO Is a Reductase for Heme Degradation by IsdI and IsdG Proteins in Staphylococcus aureus

Quantitative proteomes and in vivo secretomes of progressive and regressive UV‐induced fibrosarcoma tumor cells: Mimicking tumor microenvironment using a dermis‐based cell‐trapped system linked to tissue chamber

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